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Here, we report that Tyr-Tyr-Glu (YYE) is effective for articular cartilage regeneration through chondrogenic differentation of mesenchymal stem cells (MSCs). The expression of COL II, COMP, ACAN, and COL X at the mRNA and\/or protein levels, together with Alcian Blue and Safranin O staining of 3-dimensionally cultured cell pellets, consistently demonstrated that YYE is an effective chondrogenic promoter of MSCs. Western blot, immunofluorescence, and molecular docking studies suggested that the chondrogenic up-regulation by YYE is mediated through YYE binding to FC-1, which induces translocation of core-binding factor \u03b2 subunit (CBF\u03b2) from the cytosol into the nucleus, followed by up-regulation of runt-related transcription factor 1 (RUNX1), which is a key factor for chondrogenic differentiation of MSCs. In particular, intra-articular injection of YYE to mouse OA models led to a significant improvements of OA as indicated by lowering OARSI grades and subchondral bone plate thickness, and thus proved its articular cartilage regeneration efficacy.<\/jats:p>","DOI":"10.34133\/bmr.0272","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T17:34:06Z","timestamp":1760117646000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark_01","source":"Crossref","is-referenced-by-count":4,"title":["Tyr-Tyr-Glu Tripeptide for Regeneration of Articular Cartilage through Chondrogenic Differentiation of Mesenchymal Stem Cells"],"prefix":"10.34133","volume":"29","author":[{"given":"Ye Lin","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Chemistry and Nanoscience, Gradutate Program in Innovative Biomaterials Convergence, \rEwha Womans University, Seoul 03760, Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soyoun","family":"Um","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Nanoscience, Gradutate Program in Innovative Biomaterials Convergence, \rEwha Womans University, Seoul 03760, Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madhumita","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Nanoscience, Gradutate Program in Innovative Biomaterials Convergence, \rEwha Womans University, Seoul 03760, Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeon-Ju","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Nanoscience, Gradutate Program in Innovative Biomaterials Convergence, \rEwha Womans University, Seoul 03760, Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun-Shin","family":"Cha","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Nanoscience, Gradutate Program in Innovative Biomaterials Convergence, \rEwha Womans University, Seoul 03760, Korea."},{"name":"R&D Division, TODD Phaarm Co. 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