{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T01:01:22Z","timestamp":1791853282316,"version":"4.3.4"},"reference-count":37,"publisher":"American Association for the Advancement of Science (AAAS)","funder":[{"name":"General project of the Kunming Medical University Applied Basic Research Joint Special Fund of the Science and Technology Department of Yunnan Province","award":["202401AY070001-199"],"award-info":[{"award-number":["202401AY070001-199"]}]},{"name":"Yunnan Provincial Universities Serving Key Industries Science and Technology Special Project","award":["FWCY-BSPY2024075"],"award-info":[{"award-number":["FWCY-BSPY2024075"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82260186"],"award-info":[{"award-number":["82260186"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the First-Class Discipline Team of Kunming Medical University","award":["2024XKTDTS09"],"award-info":[{"award-number":["2024XKTDTS09"]}]},{"name":"Yunnan Provincial Clinical Medical Center for Oral Diseases","award":["2024YNLCYXZX0228"],"award-info":[{"award-number":["2024YNLCYXZX0228"]}]}],"content-domain":{"domain":["spj.science.org"],"crossmark-restriction":true},"short-container-title":["Biomater Res"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Periodontitis, a highly prevalent chronic inflammatory disease globally, faces substantial challenges in achieving periodontal tissue regeneration, necessitating the development of novel therapeutic strategies. Chinese herbal medicine-derived extracellular vesicles (CHMEVs), natural nanoscale carriers enriched with bioactive components from medicinal plants, exhibit unique therapeutic advantages in tissue repair. Here, we isolated extracellular vesicle-like particles from\n                    <jats:italic toggle=\"yes\">Paris polyphylla<\/jats:italic>\n                    var.\n                    <jats:italic toggle=\"yes\">yunnanensis<\/jats:italic>\n                    leaves (PP-L-EVLPs), a traditional Chinese medicinal herb native to Yunnan, and systematically evaluated their therapeutic potential for periodontal regeneration. PP-L-EVLPs were efficiently internalized by periodontal ligament stem cells (PDLSCs), enhancing their proliferation, migration, and osteogenic differentiation through up-regulation of ALP, RUNX2, and OPN. PP-L-EVLPs significantly suppressed the protein expression levels of lipopolysaccharide-induced interleukin-6 (IL-6) and IL-8 in PDLSCs. In a rat alveolar bone defect model, PP-L-EVLPs significantly promoted bone regeneration, as evidenced by micro-computed tomography, histology, and immunohistochemistry. Biosafety evaluations revealed no histopathological abnormalities or genotoxicity in major organs of Sprague\u2013Dawley rats treated with PP-L-EVLPs. This study is the first to confirm that PP-L-EVLPs exhibit cell migration-promoting, anti-inflammatory, and osteogenic activities with excellent biosafety, offering a novel natural nano-based therapeutic strategy for periodontitis treatment.\n                  <\/jats:p>","DOI":"10.34133\/bmr.0291","type":"journal-article","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:23:14Z","timestamp":1763684594000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark_01","source":"Crossref","is-referenced-by-count":4,"title":["<i>Paris polyphylla<\/i>\n                    var.\n                    <i>yunnanensis<\/i>\n                    Leaf-Derived Extracellular Vesicle-Like Particles Enhance Periodontal Regeneration"],"prefix":"10.34133","volume":"29","author":[{"given":"Guobin","family":"Huang","sequence":"first","affiliation":[{"name":"Yunnan Key Laboratory of Stomatology, \rKunming Medical University School and Hospital of Stomatology, Kunming, Yunnan, PR China."},{"name":"Department of Dental Research, \rKunming Medical University School and Hospital of Stomatology, Kunming, Yunnan, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anfan","family":"Liu","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory of Stomatology, \rKunming Medical University School and Hospital of Stomatology, Kunming, Yunnan, PR China."},{"name":"Department of Dental Research, \rKunming Medical University School and Hospital of Stomatology, Kunming, Yunnan, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Hu","sequence":"additional","affiliation":[{"name":"Outpatient Department, \rKunming Medical University School and Hospital of Stomatology, Kunming, Yunnan, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongqiang","family":"Yang","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory of Stomatology, \rKunming Medical University School and Hospital of Stomatology, Kunming, Yunnan, PR China."},{"name":"Department of Dental Research, \rKunming Medical University School and Hospital of Stomatology, Kunming, Yunnan, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zichao","family":"Dai","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory of Stomatology, \rKunming Medical University School and Hospital of Stomatology, Kunming, Yunnan, PR China."},{"name":"Department of Dental Research, \rKunming Medical University School and Hospital of Stomatology, Kunming, Yunnan, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, Yan\u2019an Hospital Affiliated to Kunming Medical University","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Stomatology, The First People\u2019s Hospital of Kunming","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3318-0733","authenticated-orcid":true,"given":"Hefeng","family":"Yang","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory of Stomatology, \rKunming Medical University School and Hospital of Stomatology, Kunming, Yunnan, PR China."},{"name":"Department of Dental Research, \rKunming Medical University School and Hospital of Stomatology, Kunming, Yunnan, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Li","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory of Stomatology, \rKunming Medical University School and Hospital of Stomatology, Kunming, Yunnan, PR China."},{"name":"Department of Dental Research, \rKunming Medical University School and Hospital of Stomatology, Kunming, Yunnan, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","published-online":{"date-parts":[[2025,12,9]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(19)33016-8"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.21-0625"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.38"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2024.1384344"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16627-0"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtumed.2024.01.006"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom14030312"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202302029"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2024.107062"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1039\/D3MH01030A"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2023.1215650"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202207826"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.896745"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2023.05.033"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114293"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2024.113994"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2022.e08982"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/1043471"},{"issue":"1","key":"e_1_3_4_20_2","first-page":"3","article-title":"Consensus statement on research and application of Chinese herbal medicine derived extracellular vesicles-like particles (2023 edition)","volume":"16","author":"Zhao Q","year":"2023","unstructured":"Zhao Q, Wang T, Wang H, Cao P, Jiang C, Qiao H, Peng L, Lin X, Jiang Y, Jin H, et al. Consensus statement on research and application of Chinese herbal medicine derived extracellular vesicles-like particles (2023 edition). Chin Herb Med. 2023;16(1):3\u201312.","journal-title":"Chin Herb Med"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaim.2024.101048"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells8060537"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202417810"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-024-05892-3"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2025.1480292"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2019.1596016"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-022-00460-3"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2022.102007"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13770-021-00367-8"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2024.155628"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu15092107"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2022.K.0094"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13705"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.14036"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-022-02767-6"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2024.02.005"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2023.11.020"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2020.100869"}],"container-title":["Biomaterials Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/spj.science.org\/doi\/pdf\/10.34133\/bmr.0291","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T03:05:39Z","timestamp":1765335939000},"score":1,"resource":{"primary":{"URL":"https:\/\/spj.science.org\/doi\/10.34133\/bmr.0291"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":37,"alternative-id":["10.34133\/bmr.0291"],"URL":"https:\/\/doi.org\/10.34133\/bmr.0291","relation":{},"ISSN":["2055-7124"],"issn-type":[{"value":"2055-7124","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2025-05-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-15","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"0291"}}