{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T17:33:35Z","timestamp":1787592815030,"version":"build-2736575974"},"reference-count":50,"publisher":"American Association for the Advancement of Science (AAAS)","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070859"],"award-info":[{"award-number":["32070859"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shandong Provincial Natural Science Foundation Major Basic Research Project","award":["ZR2022ZD21"],"award-info":[{"award-number":["ZR2022ZD21"]}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2020MC083"],"award-info":[{"award-number":["ZR2020MC083"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2021MH350"],"award-info":[{"award-number":["ZR2021MH350"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010029","name":"Taishan Scholar Foundation of Shandong Province","doi-asserted-by":"publisher","award":["TS20190931"],"award-info":[{"award-number":["TS20190931"]}],"id":[{"id":"10.13039\/501100010029","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science of Foundation","doi-asserted-by":"crossref","award":["2024M761564"],"award-info":[{"award-number":["2024M761564"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Chinese Postdoctoral Research Program","award":["GZC20231191"],"award-info":[{"award-number":["GZC20231191"]}]},{"name":"Science and Technology Program Development project of Qingdao city south district","award":["2023-2-020-YY"],"award-info":[{"award-number":["2023-2-020-YY"]}]},{"name":"the Science, and Education and Industry Integration Innovation Pilot Project from Qilu University of Technology","award":["2024ZDZX14"],"award-info":[{"award-number":["2024ZDZX14"]}]}],"content-domain":{"domain":["spj.science.org"],"crossmark-restriction":true},"short-container-title":["Biomater Res"],"published-print":{"date-parts":[[2026,1,1]]},"abstract":"<jats:p>Osteoarthritis (OA) is a progressive joint disorder that predominantly affects elderly and postmenopausal individuals. Current therapies offer only transient symptom relief and are associated with significant adverse effects. Mesenchymal stem cell (MSC) therapy holds promise for OA treatment, but challenges such as poor in\u00a0vivo persistence and migration away from target sites hinder its clinical application. Here, we develop a bioactive, thermosensitive hydroxypropyl chitin (HPCT) hydrogel as an injectable platform to enhance MSC-based therapy. In both papain-induced early-stage and surgically induced late-stage OA models, intra-articular injections of MSCs combined with HPCT hydrogel significantly enhance therapeutic efficacy within the osteoarthritic joint environment. This bioactive, thermosensitive hydrogel is associated with attenuation of mechanical-stress-related ferroptotic signatures in chondrocytes through the establishment of a protective biomechanical microenvironment. MSCs embedded within the hydrogel adopt a spheroidal configuration, which improves their viability, enhances their anti-inflammatory properties, and prolongs their retention at the site of injury. These combined effects promote cartilage repair, regeneration, and sustained joint homeostasis. Mechanistically, these effects are accompanied by modulation of mechanotransduction-related pathways, including reduced Piezo1 expression and restoration of GPX4-associated antioxidant capacity. Our findings highlight HPCT-based tissue engineering as a promising therapeutic strategy for addressing OA pathophysiology and improving long-term clinical outcomes.<\/jats:p>","DOI":"10.34133\/bmr.0382","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T03:32:43Z","timestamp":1779679963000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark_01","source":"Crossref","is-referenced-by-count":1,"title":["Bioactive Chitin-Based Thermosensitive Hydrogel Reinforces Stem Cell Therapy for Osteoarthritis"],"prefix":"10.34133","volume":"30","author":[{"given":"Yiming","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery, \rThe Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, China."},{"name":"College of Integrated Traditional Chinese and Western Medicine, \rJining Medical University, Jining, Shandong 272067, China."},{"name":"Department of Genetics and Cell Biology, Basic Medical College, \rQingdao University, Qingdao, Shandong 266071, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinbing","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, Basic Medical College, \rQingdao University, Qingdao, Shandong 266071, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, Basic Medical College, \rQingdao University, Qingdao, Shandong 266071, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, Basic Medical College, \rQingdao University, Qingdao, Shandong 266071, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufeng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, Basic Medical College, \rQingdao University, Qingdao, Shandong 266071, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zicheng","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, Basic Medical College, \rQingdao University, Qingdao, Shandong 266071, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weizhi","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Marine Life Sciences, \rOcean University of China, Shandong 266000, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, Basic Medical College, \rQingdao University, Qingdao, Shandong 266071, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, Basic Medical College, \rQingdao University, Qingdao, Shandong 266071, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangmin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, Basic Medical College, \rQingdao University, Qingdao, Shandong 266071, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolei","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, Basic Medical College, \rQingdao University, Qingdao, Shandong 266071, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuping","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, Basic Medical College, \rQingdao University, Qingdao, Shandong 266071, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiane","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, Basic Medical College, \rQingdao University, Qingdao, Shandong 266071, China."},{"name":"Department of Reproductive Medicine, \rThe Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunfeng","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, \rThe Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, China."},{"name":"Qingdao Anyrule Biological Health Technology Co., Ltd","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daijie","family":"Wang","sequence":"additional","affiliation":[{"name":"International Joint Laboratory of Medicinal Food R&amp;D and Health Products Creation\/Biological Engineering Technology Innovation Center of Shandong Province, \rHeze Branch of Qilu University of Technology (Shandong Academy of Sciences), Heze, Shandong 274000, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, \rThe Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoqin","family":"Han","sequence":"additional","affiliation":[{"name":"College of Marine Life Sciences, \rOcean University of China, Shandong 266000, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4471-5983","authenticated-orcid":true,"given":"Zheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, \rThe Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, China."},{"name":"Department of Genetics and Cell Biology, Basic Medical College, \rQingdao University, Qingdao, Shandong 266071, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoqi","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, \rThe Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, 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Comparative assessment of lipid peroxidase in oral cancer and oral potentially malignant disorders. 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