{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:16:42Z","timestamp":1792790202846,"version":"4.5.0"},"reference-count":49,"publisher":"American Association for the Advancement of Science (AAAS)","funder":[{"DOI":"10.13039\/501100021171","name":"Guangdong Basic and Applied Basic Research Foundation","doi-asserted-by":"crossref","award":["2023A1515220055"],"award-info":[{"award-number":["2023A1515220055"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"crossref"}]},{"name":"General Research Project of Guangzhou Health Commission","award":["20241A011115"],"award-info":[{"award-number":["20241A011115"]}]},{"name":"Panyu Science and Technology Planning Project - Key Medical and Health Project","award":[".2022-Z04-101"],"award-info":[{"award-number":[".2022-Z04-101"]}]}],"content-domain":{"domain":["spj.science.org"],"crossmark-restriction":true},"short-container-title":["Biomater Res"],"published-print":{"date-parts":[[2026,1,1]]},"abstract":"<jats:p>Steroid-associated osteonecrosis (SAON) is characterized by glucocorticoid-associated vascular compromise, impaired bone repair, and a dysregulated inflammatory microenvironment. Although core decompression (CD) remains the main joint-preserving procedure, its efficacy is often limited by the hostile local niche. Here, we engineered a hybrid CDM@Fibrin\/poly(\u03b5-caprolactone) (PCL) scaffold by incorporating human umbilical cord mesenchymal-stem-cell-derived decellularized matrix (CDM) into a 3D-printed PCL framework. Proteomic profiling showed enrichment of extracellular-matrix-associated proteins linked to focal adhesion, extracellular matrix\u2013receptor interaction, and phosphatidylinositol 3-kinase\u2013Akt-related signaling. In\u00a0vitro, solubilized CDM was biocompatible and modulated macrophage behavior in a context-dependent manner; under basal conditions, its effects on canonical polarization markers were modest, whereas under inflammatory challenge it attenuated lipopolysaccharide-induced M1-like activation and partially restored pro-healing features. In a rat femoral condyle defect model, CDM@Fibrin\/PCL enhanced bone formation and was associated with lower CD86 and relatively higher CD206 signals than Fibrin\/PCL controls. In a preclinical SAON model, scaffold-augmented CD markedly improved new bone formation and perfused vascular volume relative to CD alone. Exploratory transcriptomic analysis identified pathway-level associations related to immune regulation, extracellular matrix remodeling, and reparative signaling. Collectively, these findings suggest that mesenchymal-stem-cell-derived CDM functions as a bioactive matrix component that helps rebalance the local inflammatory niche and supports bone repair with improved vascularization-related outcomes in SAON.<\/jats:p>","DOI":"10.34133\/bmr.0383","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T17:11:31Z","timestamp":1779815491000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark_01","source":"Crossref","is-referenced-by-count":1,"title":["A Hybrid Mesenchymal-Stem-Cell-Derived Decellularized Matrix Scaffold Supports Bone Repair and Vascular Perfusion in Steroid-Associated Osteonecrosis"],"prefix":"10.34133","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5062-1866","authenticated-orcid":true,"given":"Yijun","family":"He","sequence":"first","affiliation":[{"name":"Department of Osteoarthropathy and Sports Medicine, The Affiliated Panyu Central Hospital, \rGuangzhou Medical University, Guangzhou, Guangdong 511400, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chu","family":"Hua","sequence":"additional","affiliation":[{"name":"Translational Research Centre of Regenerative Medicine and 3D Printing, Department of Orthopaedic Surgery, Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, Guangdong Province Engineering Research Center for Biomedical Engineering, State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital, \rGuangzhou Medical University, Guangzhou, Guangdong 510150, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Liang","sequence":"additional","affiliation":[{"name":"Translational Research Centre of Regenerative Medicine and 3D Printing, Department of Orthopaedic Surgery, Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, Guangdong Province Engineering Research Center for Biomedical Engineering, State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital, \rGuangzhou Medical University, Guangzhou, Guangdong 510150, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Minimally Invasive Interventional Radiology, The Affiliated Panyu Central Hospital, \rGuangzhou Medical University, Guangzhou, Guangdong 511400, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Li","sequence":"additional","affiliation":[{"name":"Translational Research Centre of Regenerative Medicine and 3D Printing, Department of Orthopaedic Surgery, Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, Guangdong Province Engineering Research Center for Biomedical Engineering, State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital, \rGuangzhou Medical University, Guangzhou, Guangdong 510150, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiongfeng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Osteoarthropathy and Sports Medicine, The Affiliated Panyu Central Hospital, \rGuangzhou Medical University, Guangzhou, Guangdong 511400, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Osteoarthropathy and Sports Medicine, The Affiliated Panyu Central Hospital, \rGuangzhou Medical University, Guangzhou, Guangdong 511400, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Yong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Translational Research Centre of Regenerative Medicine and 3D Printing, Department of Orthopaedic Surgery, Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, Guangdong Province Engineering Research Center for Biomedical Engineering, State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital, \rGuangzhou Medical University, Guangzhou, Guangdong 510150, PR 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