{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:56:27Z","timestamp":1790758587263,"version":"4.1.0"},"reference-count":83,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"1","funder":[{"DOI":"10.13039\/501100014438","name":"Business Finland","doi-asserted-by":"publisher","award":["9392\/31\/2021"],"award-info":[{"award-number":["9392\/31\/2021"]}],"id":[{"id":"10.13039\/501100014438","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002341","name":"Academy of Finland","doi-asserted-by":"publisher","award":["348628"],"award-info":[{"award-number":["348628"]}],"id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["spj.science.org"],"crossmark-restriction":true},"short-container-title":["Comput Struct Biotechnol J"],"published-print":{"date-parts":[[2026,3,1]]},"abstract":"<jats:p>\n                    Silk-inspired proteins are attractive biomacromolecules owing to their structural diversity, sustainability, and tunable physicochemical properties. Among natural silks, the bagworm silk produced by\n                    <jats:italic toggle=\"yes\">Eumeta variegata<\/jats:italic>\n                    has attracted increasing attention because of its unique sequence architecture and remarkable reported mechanical performance. However, the recombinant production of bagworm silk-inspired proteins remains largely unexplored. In this study, we designed and heterologously expressed 2 bagworm silk-inspired protein variants in\n                    <jats:italic toggle=\"yes\">Aspergillus oryzae<\/jats:italic>\n                    using a synthetic expression system. The variants comprised a representative bagworm silk with an alanine- and glycine-rich repeat motif flanked by carbohydrate-binding modules at the N- and C-termini. Expression screening in engineered\n                    <jats:italic toggle=\"yes\">A. oryzae<\/jats:italic>\n                    strains demonstrated stable accumulation of C-tag-reactive species corresponding to both variants in a protease-deficient host, whereas comparable accumulation was not detected in a carbon-catabolite-repressor-deficient strain under the tested conditions. The selected variant was further produced in a 2-l bioreactor, demonstrating the feasibility of scale-up and establishing\n                    <jats:italic toggle=\"yes\">A. oryzae<\/jats:italic>\n                    as a promising platform for bagworm silk-inspired protein production. The recovered H-eBGW-containing protein fractions were readily processed into thin films and exhibited temperature-dependent changes in mechanical properties and surface wettability, demonstrating the processability and curing-responsive behavior of the recovered material. Collectively, our results demonstrate the feasibility of producing structurally complex bagworm silk-inspired proteins in filamentous fungi and provide insights into the design, expression, and processing of recombinant silk-inspired proteins for future bio-based applications.\n                  <\/jats:p>","DOI":"10.34133\/csbj.0233","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T22:10:58Z","timestamp":1790115058000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark_01","source":"Crossref","is-referenced-by-count":0,"title":["Design and Heterologous Production of Bagworm Silk-Inspired Proteins in\n                    <i>Aspergillus oryzae<\/i>"],"prefix":"10.34133","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7208-507X","authenticated-orcid":true,"given":"Adiphol","family":"Dilokpimol","sequence":"first","affiliation":[{"name":"VTT Technical Research Center of Finland","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrike","family":"Abendroth","sequence":"additional","affiliation":[{"name":"VTT Technical Research Center of Finland","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari","family":"Nyyss\u00f6nen","sequence":"additional","affiliation":[{"name":"VTT Technical Research Center of Finland","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3414-9888","authenticated-orcid":false,"given":"Anna S.","family":"Borisova","sequence":"additional","affiliation":[{"name":"VTT Technical Research Center of Finland","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anni","family":"Seisto","sequence":"additional","affiliation":[{"name":"VTT Technical Research Center of Finland","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenni","family":"Muli","sequence":"additional","affiliation":[{"name":"VTT Technical Research Center of Finland","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6078-4503","authenticated-orcid":false,"given":"Dominik","family":"Mojzita","sequence":"additional","affiliation":[{"name":"VTT Technical Research Center of Finland","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6441-8058","authenticated-orcid":false,"given":"Fiona","family":"Sm\u00e5ros","sequence":"additional","affiliation":[{"name":"VTT Technical Research Center of Finland","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0107-1938","authenticated-orcid":false,"given":"Hans","family":"Mattila","sequence":"additional","affiliation":[{"name":"VTT Technical Research Center of Finland","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3093-2493","authenticated-orcid":false,"given":"Antti P.","family":"Aalto","sequence":"additional","affiliation":[{"name":"VTT Technical Research Center of Finland","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari","family":"Valkonen","sequence":"additional","affiliation":[{"name":"VTT Technical Research Center of Finland","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4593-5371","authenticated-orcid":true,"given":"Pezhman","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"VTT Technical Research Center of Finland","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.2c00621"},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","DOI":"10.1016\/j.clema.2024.100220","article-title":"The world of plastic waste: A review","volume":"11","author":"Pilapitiya PN","year":"2024","unstructured":"Pilapitiya PN, Ratnayake AS. 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