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Navigation technique was used to make neutrophils rapidly infiltrate into the tumor tissue, which played a key role in inhibiting tumor growth. We found that tumor cells were efficiently killed in direct coculture with sufficient neutrophils by promoting a multimodal death primarily dominated by apoptosis. Interestingly, colon cancer organoid diameters were decreased markedly cocultured with neutrophils, and were more remarkable in the application of organoid microinjection of neutrophils. Consistent with the 3D-printed model, in\u00a0vivo model indicated that neutrophils meaningfully inhibited the tumor growth by formation of neutrophil extracellular traps (NETs) with neutrophil elastase (NE) payload. This study not only unveils the crucial anticancer effects of neutrophils but also sets out an innovative strategy of aggressive dose exogenous neutrophils with precise navigation, which provided the basis for advancements in neutrophil-based immunotherapy for colon cancer treatment.<\/jats:p>","DOI":"10.34133\/research.0894","type":"journal-article","created":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T12:07:42Z","timestamp":1756123662000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark_01","source":"Crossref","is-referenced-by-count":1,"title":["Highly Selective Exogenous Neutrophils Effectively Inhibit Growth of Colon Cancer under the Guidance of Precision Navigation"],"prefix":"10.34133","volume":"8","author":[{"given":"Yunxi","family":"Yang","sequence":"first","affiliation":[{"name":"Research Center for Neutrophil Engineering Technology, \rThe Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215002, Jiangsu Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuyu","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, \rThe First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiming","family":"Shao","sequence":"additional","affiliation":[{"name":"Research Center for Neutrophil Engineering Technology, \rThe Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215002, Jiangsu Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Wen","sequence":"additional","affiliation":[{"name":"Research Center for Neutrophil Engineering Technology, \rThe Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215002, Jiangsu Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Lu","sequence":"additional","affiliation":[{"name":"Research Center for Neutrophil Engineering Technology, \rThe Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215002, Jiangsu Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Gao","sequence":"additional","affiliation":[{"name":"Research Center for Neutrophil Engineering Technology, \rThe Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215002, Jiangsu Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwen","family":"Mei","sequence":"additional","affiliation":[{"name":"Research Center for Neutrophil Engineering Technology, \rThe Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215002, Jiangsu Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7083-8525","authenticated-orcid":true,"given":"Bingwei","family":"Sun","sequence":"additional","affiliation":[{"name":"Research Center for Neutrophil Engineering Technology, \rThe Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215002, Jiangsu Province, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","published-online":{"date-parts":[[2025,9,16]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1180810","article-title":"Neutrophil diversity in inflammation and cancer","volume":"14","author":"Carnevale S","year":"2023","unstructured":"Carnevale S, Di Ceglie I, Grieco G, Rigatelli A, Bonavita E, Jaillon S. 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