{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T22:07:22Z","timestamp":1740175642477,"version":"3.37.3"},"reference-count":30,"publisher":"Maad Rayan Publishing Company","issue":"4","content-domain":{"domain":["apb.tbzmed.ac.ir"],"crossmark-restriction":false},"short-container-title":["Adv Pharm Bull"],"published-print":{"date-parts":[[2020,7,26]]},"abstract":"<jats:p> Purpose: Previous studies have documented that cumulus granulosa cells (GCs) can trans-differentiation into different non-ovarian cells, showing their multipotentiality to repopulate the injured cells in ovarian tissue. The current experiment is aimed to assess the differentiation capacity of human cumulus GCs toward the oocyte-like phenotype in vitro. Methods: GCs were isolated from healthy female volunteers subjected to in vitro fertilization or intra-cytoplasmic sperm injection (IVF-ICSI). The effect of different media supplemented with leukemia inhibitory factors (LIFs), 5 ng\/mL estradiol, and 0.005 IU\/mL follicle-stimulating hormone (FSH) were investigated to the differentiation of GCs toward oocyte-like phenotype via monitoring the expression of Oct3\/4 and GATA-4 using flow cytometry analysis. The expression of genes such as FIGLA, NOBOX, and SYCP3 was measured by real-time polymerase chain reaction (PCR) assay. We also assess morphological adaptation by using bright-field microscopic imaging. Results: Exposure of GCs to LIFs increased the number of cells expressing stemness factor Oct3\/4 coincided with the suppression of GATA-4 after 7 days (P&lt;0.05). We found that the transcript level of all genes FIGLA, Nobox, and SYCP-3 decreased in cells after treatment with a FSH (P&lt;0.05). According to our data, the incubation of GCs with estradiol increased the expression of genes related to the oocyte-like phenotype. Conclusion: Our finding revealed that the combination of LIFs and estradiol could induce the GCs\u2019 oogenesis capacity and thereby is possibly suggested as a therapeutic strategy during the occurrence of gynecological disorders. <\/jats:p>","DOI":"10.34172\/apb.2021.080","type":"journal-article","created":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T05:01:09Z","timestamp":1610514069000},"page":"712-718","update-policy":"https:\/\/doi.org\/10.34172\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Combination of Estradiol with Leukemia Inhibitory Factor Stimulates Granulosa Cells Differentiation into Oocyte-Like Cells"],"prefix":"10.34172","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5668-2248","authenticated-orcid":true,"given":"Soudabe","family":"Yousefi","sequence":"first","affiliation":[{"name":"Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran."},{"name":"Department of Biochemistry and Clinical 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