{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T19:56:05Z","timestamp":1780602965217,"version":"3.54.1"},"reference-count":0,"publisher":"Maad Rayan Publishing Company","content-domain":{"domain":["bi.tbzmed.ac.ir"],"crossmark-restriction":false},"short-container-title":["Bioimpacts"],"published-print":{"date-parts":[[2021,9,25]]},"abstract":"<jats:p>Introduction: Cell-based therapies with certain cell types are touted as novel and hopeful therapeutic intervention in the clinical setting. Here, we aimed to assess the regenerative potential of c-Kit+ cells in the rejuvenation of ovarian tissue and fertility rate in rat model of premature ovarian failure (POF).  Methods: Rats were treated with 160 mg\/kg\/BW of 4-vinylcyclohexene dioxide for 15 days. Freshly enriched rat bone marrow-derived c-Kit+ (MACS) and c-Kit- cells (4\u00d7105 cells\/10 \u00b5L) were transplanted into the ovaries of treatment and control animals. Prior to transplantation as well as 2, 4, 6, and 8 weeks post-transplantation, randomly-selected rats were euthanized and ovarian tissues were subjected to pathophysiological examinations and real-time PCR analyses.  Results: POF status was confirmed by the presence of pathological features and a decreased number of immature and mature follicles compared with the control group (P\u2003&lt;\u20030.05). Histological examination revealed a substantial reduction of atretic follicles in POF rats receiving c-Kit+ cells in comparison with POF rats that did not receive these cells (P\u2003&lt;\u20030.05). Compared with the control samples, angiogenesis-related genes, Angpt2 and KDR, showed increased and decreased expressions in POF ovaries, respectively (P\u2003&lt;\u20030.05). c-Kit+ cells had potential to restore angiogenesis in the ovarian tissue within normal ranges. Systemic levels of FSH did not significantly change in pre- or post-transplantation time points for any group (P\u2003&gt;\u20030.05). Notable reduction of collagen deposition was found in c-Kit-treated rats. Transplantation of c-Kit+ cells also restored the reduced fertility rate (P\u2003&lt;\u20030.05).  Conclusion: The administration of c-Kit+ cells can modulate angiogenesis and pathological changes, leading to the rejuvenation of ovarian function of a rat model of premature menopause.<\/jats:p>","DOI":"10.34172\/bi.2021.23499","type":"journal-article","created":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T11:51:41Z","timestamp":1633002701000},"update-policy":"https:\/\/doi.org\/10.34172\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<sup>\n            <\/sup>Intra-ovarian injection of bone marrow-derived c-Kit<sup>+<\/sup> cells for ovarian rejuvenation in menopausal rats"],"prefix":"10.34172","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8590-8111","authenticated-orcid":true,"given":"Sepideh","family":"Sheshpari","sequence":"first","affiliation":[{"name":"Department of Midwifery, Faculty of Nursing and Midwifery, Tabriz University of Medical Sciences, Tabriz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahnaz","family":"Shahnazi","sequence":"additional","affiliation":[{"name":"Department of Midwifery, Faculty of Nursing and Midwifery, Tabriz University of Medical Sciences, Tabriz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahin","family":"Ahmadian","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, Azerbaijan Shahid Madani University, Tabriz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Nouri","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran"},{"name":"Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehran","family":"Mesgari Abbasi","sequence":"additional","affiliation":[{"name":"Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahim","family":"Beheshti","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, Islamic Azad University, Shabester Branch, Shabestar, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Rahbarghazi","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran"},{"name":"Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Honaramooz","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, S7N 5B4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2729-4593","authenticated-orcid":true,"given":"Mahdi","family":"Mahdipour","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran"},{"name":"Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"20123","published-online":{"date-parts":[[2021,9,25]]},"container-title":["BioImpacts"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bi.tbzmed.ac.ir\/Inpress\/bi-23499.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bi.tbzmed.ac.ir\/Inpress\/bi-23499.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T11:51:41Z","timestamp":1633002701000},"score":1,"resource":{"primary":{"URL":"https:\/\/bi.tbzmed.ac.ir\/Inpress\/bi-23499"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,25]]},"references-count":0,"URL":"https:\/\/doi.org\/10.34172\/bi.2021.23499","relation":{},"ISSN":["2228-5660","2228-5652"],"issn-type":[{"value":"2228-5660","type":"print"},{"value":"2228-5652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,25]]},"assertion":[{"value":"Tabriz University of Medical Sciences","name":"journal_owner","label":"Journal Owner"},{"value":"Tabriz University of Medical Sciences","name":"journal_publisher","label":"Journal Publisher"},{"value":"2020-11-07","order":1,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}