{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T22:59:25Z","timestamp":1777417165885,"version":"3.51.4"},"reference-count":50,"publisher":"Maad Rayan Publishing Company","issue":"3","content-domain":{"domain":["bi.tbzmed.ac.ir"],"crossmark-restriction":false},"short-container-title":["Bioimpacts"],"published-print":{"date-parts":[[2020,7,8]]},"abstract":"<jats:p>Introduction: Acute kidney injury (AKI) induced by renal ischemia-reperfusion (I\/R) injury is a pro-inflammatory process that activates toll-like receptors (TLRs). Stem cell therapy holds a great promise for kidney repair. Therefore, we investigated the immunomodulatory role of bone marrow stromal cells (BMSCs) on TLR2 and TLR4 expression in AKI in male Sprague-Dawley rats. Methods: BMSCs were isolated from the bone marrow of male rats, cultured in DMEM, and characterized using appropriate markers before transplantation. Renal I\/R was induced by 45 minutes bilateral ischemia followed by 24 hours of reperfusion. Rats received intraperitoneal injections of BMSCs (1.5 \u00d7 106 cells, i.p, per rat) immediately after termination of renal ischemia. Serum samples were collected pre-and post-stem cells injection for assessment of blood urea nitrogen (BUN) and creatinine (Cr) levels. The kidneys were harvested after 24 hours of reperfusion for structural and molecular analysis. Results: Renal I\/R caused severe tissue injuries and increased the level of BUN (166.5 \u00b1 12.9 vs. 18.25\u2003\u00b1\u20031.75) and Cr (3.7\u2003\u00b1\u20030.22 vs. 0.87\u2003\u00b1\u20030.06) compared to the sham group. In addition, mRNA expression of TLR2 and TLR4 elevated in the renal I\/R group. Administration of BMSCs improved the functional and structural state of the kidney induced by I\/R and down-regulated TLR2 and TLR4 gene expression. Conclusion: The results showed a highly significant renoprotection by BMSCs that indicates their therapeutic potential in I\/R injures. These effects are most likely associated with the TLR2\/4 signaling pathway via modulation of the inflammatory response cascades.<\/jats:p>","DOI":"10.34172\/bi.2021.31","type":"journal-article","created":{"date-parts":[[2021,7,6]],"date-time":"2021-07-06T15:29:40Z","timestamp":1625585380000},"page":"219-226","update-policy":"https:\/\/doi.org\/10.34172\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Therapeutic effects of bone marrow mesenchymal stem cells via modulation of TLR2 and TLR4 on renal ischemia-reperfusion injury in male Sprague-Dawley rats"],"prefix":"10.34172","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9558-099X","authenticated-orcid":true,"given":"Zeinab","family":"Karimi","sequence":"first","affiliation":[{"name":"Shiraz Nephro-Urology Research Center (SNURC), Shiraz University of Medical Sciences, Shiraz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sahar","family":"Janfeshan","sequence":"additional","affiliation":[{"name":"Shiraz Nephro-Urology Research Center (SNURC), Shiraz University of Medical Sciences, Shiraz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elias","family":"Kargar Abarghouei","sequence":"additional","affiliation":[{"name":"Department of Anatomical Sciences, Faculty of Medicine, Hormozgan University of Medical Sciences, Hormozgan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9395-9573","authenticated-orcid":true,"given":"Seyedeh-Sara","family":"Hashemi","sequence":"additional","affiliation":[{"name":"Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"20123","published-online":{"date-parts":[[2020,7,8]]},"reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-009-0491-y"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2008.08.016"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1996.271.2.F408"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1159\/000049032"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.022"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118498"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0304127"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.10.6252"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-009-1422-4"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2006.09.008"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5411.143"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5309.71"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.22038\/IJBMS.2017.19783.5212"},{"key":"R14","first-page":"547","article-title":"Potential of human umbilical cord blood mesenchymal stem cells to heal damaged corneal endothelium","volume":"18","author":"Joyce NC","year":"2012","journal-title":"Mol Vis"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.22038\/ijbms.2020.34324.8156"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1834138100"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20886"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-04-1559"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.23876\/j.krcp.19.051"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1159\/000499272"},{"key":"R21","doi-asserted-by":"crossref","first-page":"s45","DOI":"10.1590\/1806-9282.66.s1.45","article-title":"Mesenchymal stem cell therapy in acute kidney injury (AKI): review and perspectives","volume":"66","author":"S\u00e1vio-Silva C","year":"2020","journal-title":"Rev Assoc Med Bras (1992)"},{"key":"R22","doi-asserted-by":"crossref","unstructured":"Ahdjoudj S, Lasmoles F, Oyajobi BO, Lomri A, Delannoy P, Marie PJ. 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