{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T18:18:41Z","timestamp":1781893121223,"version":"3.54.5"},"reference-count":41,"publisher":"Maad Rayan Publishing Company","issue":"1","content-domain":{"domain":["bi.tbzmed.ac.ir"],"crossmark-restriction":false},"short-container-title":["Bioimpacts"],"published-print":{"date-parts":[[2023,1,1]]},"abstract":"<jats:p>Introduction: The mixed flavonoid supplement (MFS) [Trimethoxy Flavones (TMF) + epigallocatechin-3-gallate (EGCG)] can be used to suppress inflammatory ulcers as an ethical medicine in Ayurveda. The inflammation of the rectum and anal regions is mostly attributed to nuclear factor kappa beta (NF-\u03baB) signaling. NF-\u03baB stimulates the expression of matrix metalloproteinase (MMP9), inflammatory cytokines tumor necrosis factor (TNF-\u03b1), and interleukin-1\u03b2 (IL-1\u03b2). Although much research targeted the NF-\u03baB and MMP9 signaling pathways, a subsequent investigation of target mediators in the inflammatory ulcer healing and NF-\u03baB pathway has not been done. Methods: The docking studies of compounds TMF and EGCG were performed by applying PyRx and available software to understand ligand binding properties with the target proteins. The synergistic ulcer healing and anti-arthritic effects of MFS were elucidated using dextran sulfate sodium (DSS)-induced colon ulcer in Swiss albino rats. The colon mucosal injury was analyzed by colon ulcer index (CUI) and anorectic tissue microscopy. The IL-1\u03b2, tumor necrosis factor (TNF-\u03b1), and the pERK, MMP9, and NF-\u03baB expressions in the colon tissue were determined by ELISA and Western blotting. RT-PCR determined the mRNA expression for inflammatory marker enzymes. Results: The docking studies revealed that EGCG and TMF had a good binding affinity with MMP9 (i.e., -6.8 and -6.0 Kcal\/mol) and NF-kB (-9.4 and 8.3 kcal\/mol). The high dose MFS better suppressed ulcerative colitis (UC) and associated arthritis with marked low-density pERK, MMP9, and NF-\u03baB proteins. The CUI score and inflammatory mediator levels were suppressed with endogenous antioxidant levels in MFS treated rats. Conclusion: The MFS effectively unraveled anorectic tissue inflammation and associated arthritis by suppressing NF-\u03baB-mediated MMP9 and cytokines.<\/jats:p>","DOI":"10.34172\/bi.2022.23523","type":"journal-article","created":{"date-parts":[[2023,1,8]],"date-time":"2023-01-08T18:04:05Z","timestamp":1673201045000},"page":"73-84","update-policy":"https:\/\/doi.org\/10.34172\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Inhibitory effects of mixed flavonoid supplements on unraveled DSS-induced ulcerative colitis and arthritis"],"prefix":"10.34172","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6980-6761","authenticated-orcid":true,"given":"Siva Prasad","family":"Panda","sequence":"first","affiliation":[{"name":"Pharmacology Research Division, Institute of Pharmaceutical Research, GLA University, Mathura, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahamat  Sami Adam","family":"Mahamat","sequence":"additional","affiliation":[{"name":"Pharmacognosy Research Division, College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malikyahia Abdul","family":"Rasool","sequence":"additional","affiliation":[{"name":"Pharmacognosy Research Division, College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DSNBK","family":"Prasanth","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada, AP, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Idris Adam","family":"Ismail","sequence":"additional","affiliation":[{"name":"Pharmacognosy Research Division, College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moyed Abasher Ahmed","family":"Abasher","sequence":"additional","affiliation":[{"name":"Pharmacognosy Research Division, College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bikash Ranjan","family":"Jena","sequence":"additional","affiliation":[{"name":"SIIMS College of Pharmacy, Guntur, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"20123","published-online":{"date-parts":[[2022,12,31]]},"reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Mukherjee P, Wahile A. 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