{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T21:45:58Z","timestamp":1740174358094,"version":"3.37.3"},"reference-count":0,"publisher":"Maad Rayan Publishing Company","content-domain":{"domain":["bi.tbzmed.ac.ir"],"crossmark-restriction":false},"short-container-title":["Bioimpacts"],"abstract":"<jats:p>Introduction: Despite the efforts to contain the spread of COVID-19, the virus remains in circulation, posing a considerable risk to populations across the globe. Hence, rapid and early detection of this infection is essential for effective disease control. The nucleocapsid (N) protein of the virus serves as a primary target for antibody response during CoV2 infections, making it a potential candidate for COVID-19 detection. This study aims to prepare and evaluate the linear epitopes of the N protein for serodiagnosis of COVID-19 infection.  Methods: The linear epitope of the N protein gene was identified using ABCpred, BCpred, and IEDB. These epitopes were subsequently amplified by RT-PCR, cloned, and expressed in soluble form in the E. coli BL21 strain. The recombinant protein was purified using the Ni-NTA column. The reactivity of purified N protein with sera from SARS-CoV-2 patients was analyzed using an ELISA assay. Results: Sequencing analysis demonstrated the successful cloning of the linear epitopes of the N protein into the PET-28a vector, along with an n-terminal His-tag fusion. The recombinant protein was produced in E. coli BL21 and purified with a Ni-NTA column. The analysis demonstrated that the N protein linear epitopes were expressed in a soluble form and appeared as a 50 kDa band in the SDS-PAGE. Examination for the reactivity of the purified N protein with the COVID-19 patient\u2019s sera by ELISA revealed that the N protein recognizes the infection with high sensitivity and specificity. Conclusion: The results of this study indicated that linear epitopes of the SARS-CoV-2 N protein are highly immunogenic and could be exploited for serodiagnosis of infection in patients suspected of COVID-19 infection. <\/jats:p>","DOI":"10.34172\/bi.30232","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T08:23:15Z","timestamp":1724055795000},"update-policy":"https:\/\/doi.org\/10.34172\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Performance of protein N linear epitopes in serodiagnosis of COVID-19 infection"],"prefix":"10.34172","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6622-9014","authenticated-orcid":true,"given":"Sahar","family":"Farajnia","sequence":"first","affiliation":[{"name":"Department of Biology, University of Rabe Rashidi, Tabriz, Iran"},{"name":"Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nazli","family":"Khajenasiri","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rabe Rashidi, Tabriz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6087-9147","authenticated-orcid":true,"given":"Safar","family":"Farajnia","sequence":"additional","affiliation":[{"name":"Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran"},{"name":"Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farzin","family":"Seyrafi","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nasim","family":"Bakhtiyari","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran"},{"name":"Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"20123","published-online":{"date-parts":[[2024,6,26]]},"container-title":["BioImpacts"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bi.tbzmed.ac.ir\/Inpress\/bi-30232.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bi.tbzmed.ac.ir\/Inpress\/bi-30232.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T08:23:16Z","timestamp":1724055796000},"score":1,"resource":{"primary":{"URL":"https:\/\/bi.tbzmed.ac.ir\/Inpress\/bi-30232"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,26]]},"references-count":0,"URL":"https:\/\/doi.org\/10.34172\/bi.30232","relation":{},"ISSN":["2228-5652","2228-5660"],"issn-type":[{"type":"print","value":"2228-5652"},{"type":"electronic","value":"2228-5660"}],"subject":[],"published":{"date-parts":[[2024,6,26]]},"assertion":[{"value":"Tabriz University of Medical Sciences","name":"journal_owner","label":"Journal Owner"},{"value":"Tabriz University of Medical Sciences","name":"journal_publisher","label":"Journal Publisher"},{"value":"2023-12-20","order":1,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}