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Key prognostic genes include PGK1, PFKP, DDX39A, RFC4, WAS, and PFKM, with PFKP identified as a risk factor.  Results: The risk model demonstrated strong predictive performance, and immune infiltration analysis revealed elevated levels of immune cells, with CD8+ T cells negatively correlating with risk. ScRNA-seq analysis identified three distinct cell types, with CD8+ T cells showing a distinct developmental trajectory, marked by a reduction in early-stage cells and an accumulation of late-stage cells within the CESC microenvironment. Moreover, the expression of prognostic genes was elevated during the later stages of CD8+ T cell differentiation.  Conclusion: LRGs demonstrated significant prognostic value in CESC and accurately predicted patient outcomes. Furthermore, the study underscored the pivotal role of CD8+ T cells in the progression of CESC.<\/jats:p>","DOI":"10.34172\/bi.33527","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T16:32:09Z","timestamp":1789489929000},"page":"33527","update-policy":"https:\/\/doi.org\/10.34172\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptomics and single-cell RNA sequencing uncover prognostic characteristics and immune regulatory mechanisms of lactylation in cervical cancer"],"prefix":"10.34172","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6363-5108","authenticated-orcid":true,"given":"Yunuo","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Central Laboratory, Xuzhou Central Hospital Southeast University, Xuzhou Central Hospital, Xuzhou, 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