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Subsequently, in-vitro maturation (IVM) was evaluated. Real-time PCR was used to evaluate miRNA-21,16,19,24,30,106,155 and GAPDH. Afterward, oocyte glutathione (GSH) and nucleus acetylation were measured by H4K12. Results: The expression of the miR-16, miRNA-19, miRNA-24, miRNA-106 and miRNA-155 genes in the EXO and PBMT groups was significantly down-regulated in comparison to the control group, but the expression of miRNA-21 and miR-30 significantly increased in the EXO and PBMT groups in comparison to the control group. The EXO and PBMT significantly increased GSH and nucleus acetylation (P&lt;0.0001). Conclusion: The results of this study showed that the use of EXO and PBMT can improve GSH and nucleus acetylation in the PCOS oocyte and also change the expression of miRNAs.<\/jats:p>","DOI":"10.34172\/jlms.2024.10","type":"journal-article","created":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T08:01:16Z","timestamp":1717056076000},"page":"e10","update-policy":"https:\/\/doi.org\/10.34172\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exosome Therapy and Photobiomodulation Therapy Regulate mi-RNA 21, 155 Expressions, Nucleus Acetylation and Glutathione in a Polycystic Ovary Oocyte: An In Vitro Study"],"prefix":"10.34172","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7914-065X","authenticated-orcid":true,"given":"Samira","family":"Sahraeian","sequence":"first","affiliation":[{"name":"Department of Biology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, 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