{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:19:06Z","timestamp":1792095546341,"version":"4.4.0"},"reference-count":33,"publisher":"Maad Rayan Publishing Company","content-domain":{"domain":["journals.sbmu.ac.ir"],"crossmark-restriction":false},"short-container-title":["J Lasers Med Sci"],"published-print":{"date-parts":[[2025,8,20]]},"abstract":"<jats:p>Introduction: In vivo modeling of intraocular neoplasms remains challenging due to the limitations of conventional methods, such as the poor viability of xenografted B16 melanoma cells, the need for immunosuppression, and discrepancies between histopathological features and those of human uveal melanoma. Developing a biologically relevant, reproducible model for transscleral photodynamic therapy (TS-PDT) using chlorin-based photosensitizers is essential, particularly to assess radiation penetration depth and the safety profile of the method. Methods: A model of an intraocular mass was created using an alloplant\u2014an acellular biomaterial derived from the kidney capsule\u2014implanted into the suprachoroidal space of rabbit eyes in both pigmented and non-pigmented variants. TS-PDT was performed using chlorin e6 (2.5 mg\/kg) and a 660 nm laser (0.17 W, 10 minutes). Monitoring included ultrasound imaging, fundus photography, and histological analysis conducted 41 days post-treatment. Results: The alloplant maintained structural integrity and elicited only minimal inflammatory response. The absence of thermal damage to the sclera confirmed the safety of the selected treatment parameters. In the choroid, focal thrombosis, vascular ectasia, and stromal fibrosis were observed. Retinal necrosis overlying the alloplant (penetration depth&gt;3 mm) indicated sufficient radiation penetration. The pigmented model demonstrated accumulations of pigment-laden macrophages, replicating the optical properties of melanin-containing tissues. Conclusion: This alloplant-based model enables standardized TS-PDT investigation with clinical relevance and confirmed safety. The selective effect on deep ocular tissues supports TS-PDT\u2019s potential in eye-preserving tumor therapy. Future directions include studying neovascularization and optimizing treatment protocols.<\/jats:p>","DOI":"10.34172\/jlms.2025.26","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T10:48:02Z","timestamp":1764240482000},"page":"e26","update-policy":"https:\/\/doi.org\/10.34172\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Transscleral Photodynamic Therapy with a Chlorin e6 Photosensitizer in a Rabbit Experimental Model of an Intraocular Mass Lesion"],"prefix":"10.34172","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7413-7478","authenticated-orcid":true,"given":"Ernest V.","family":"Boiko","sequence":"first","affiliation":[{"name":"Department of Scientific and Education,Fyodorov \u201cEye Microsurgery\u201d Federal State Institution, St. Petersburg Branch, Saint Petersburg, Russian Federation"},{"name":"Department of Ophthalmology, Federal State Budgetary Educational Institution of Higher Professional Education \u201cNorth-Western State Medical University named after I.I. Mechnikov,\u201d Saint Petersburg, Russian Federation"},{"name":"Medical Faculty, Department of Ophthalmology, Federal State Budgetary Educational Institution of Higher Professional Education \u201cSaint Petersburg State University,\u201d Saint Petersburg, Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5573-5712","authenticated-orcid":true,"given":"Elena V.","family":"Samkovich","sequence":"additional","affiliation":[{"name":"Department of Scientific and Education,Fyodorov \u201cEye Microsurgery\u201d Federal State Institution, St. Petersburg Branch, Saint Petersburg, Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7443-4555","authenticated-orcid":true,"given":"Irina E.","family":"Panova","sequence":"additional","affiliation":[{"name":"Department of Scientific and Education,Fyodorov \u201cEye Microsurgery\u201d Federal State Institution, St. Petersburg Branch, Saint Petersburg, Russian Federation"},{"name":"Department of Ophthalmology, Federal State Budgetary Educational Institution of Higher Professional Education \u201cNorth-Western State Medical University named after I.I. Mechnikov,\u201d Saint Petersburg, Russian Federation"},{"name":"Medical Faculty, Department of Ophthalmology, Federal State Budgetary Educational Institution of Higher Professional Education \u201cSaint Petersburg State University,\u201d Saint Petersburg, Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1397-3915","authenticated-orcid":true,"given":"Sergey B.","family":"Shevchenko","sequence":"additional","affiliation":[{"name":"Department of Physiology of Visceral Systems, Federal State Budgetary Scientific Institution \u201cInstitute of Experimental Medicine,\u201d Saint Petersburg, Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7817-9069","authenticated-orcid":true,"given":"Sergey L.","family":"Vorobyev","sequence":"additional","affiliation":[{"name":"National Center for Clinical Morphological Diagnostics, Saint Petersburg, Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9893-7560","authenticated-orcid":true,"given":"Elizaveta S.","family":"Kalashnikova","sequence":"additional","affiliation":[{"name":"National Center for Clinical Morphological Diagnostics, Saint Petersburg, Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7495-1181","authenticated-orcid":true,"given":"Elizaveta A.","family":"Masian","sequence":"additional","affiliation":[{"name":"Department of Scientific and Education,Fyodorov \u201cEye Microsurgery\u201d Federal State Institution, St. Petersburg Branch, Saint Petersburg, Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2765-0332","authenticated-orcid":true,"given":"Victoria G.","family":"Gvazava","sequence":"additional","affiliation":[{"name":"Department of Scientific and Education,Fyodorov \u201cEye Microsurgery\u201d Federal State Institution, St. Petersburg Branch, Saint Petersburg, Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"20123","published-online":{"date-parts":[[2025,8,20]]},"reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trecan.2021.06.010"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cancerbio-070620-105523"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1159\/000370152"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.21037\/aes-21-30"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.7150\/jca.53954"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.18.2.125.5380"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/4521807"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1097\/00008390-200209000-00005"},{"key":"R9","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1097\/00008390-199306000-00008","article-title":"Ocular melanoma: an experimental model in New Zealand white rabbits","volume":"3","author":"Rodriguez Vicente J","year":"1993","journal-title":"Melanoma Res"},{"key":"R10","unstructured":"Belyi YuA, Tereshchenko AV, Volodin PL, Kaplan MA. 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