{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T09:50:44Z","timestamp":1792057844224,"version":"4.4.0"},"reference-count":23,"publisher":"Maad Rayan Publishing Company","content-domain":{"domain":["journals.sbmu.ac.ir"],"crossmark-restriction":false},"short-container-title":["J Lasers Med Sci"],"published-print":{"date-parts":[[2026,9,9]]},"abstract":"<jats:p>Introduction: Non-obstructive azoospermia (NOA) is a challenging male infertility disorder with limited treatment options. This study investigated the efficacy of novel reconstructive approaches, specifically platelet-rich fibrin (PRF), with and without laser needling, in improving testicular function and sperm retrieval in patients with NOA. Methods: In a randomized clinical trial, 40 men with NOA were divided into two groups: PRF + laser and PRF alone. Semen parameters (volume, concentration, motility, morphology, DNA fragmentation), hormone levels (FSH, LH, testosterone), and testicular volume were assessed at baseline, immediately post-intervention, and at one and three months. Results: Sperm concentration, motility, morphology, and DNA fragmentation rates showed no significant intergroup differences. While semen volume increased within groups post-intervention (P=0.039), this change was not significant between groups (P&gt;0.05). No serious complications were reported. Conclusion: In summary, this study found that PRF injection, with or without laser-acupuncture, did not significantly improve sperm parameters in NOA patients in the short term. Further research with larger cohorts, extended follow-up, and precise patient selection is needed.<\/jats:p>","DOI":"10.34172\/jlms.52024","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T09:41:52Z","timestamp":1792057312000},"page":"e52024","update-policy":"https:\/\/doi.org\/10.34172\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Effect of Platelet-Rich Fibrin (PRF) Protocol and Laser Acupuncture in the Treatment of Infertile Men with Non-Obstructive Azoospermia: A Randomized Clinical Trial"],"prefix":"10.34172","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3022-1547","authenticated-orcid":true,"given":"Mohammadreza","family":"Razaghi","sequence":"first","affiliation":[{"name":"Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, 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