{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T07:28:55Z","timestamp":1768721335794,"version":"3.49.0"},"reference-count":35,"publisher":"Maad Rayan Publishing Company","issue":"2","content-domain":{"domain":["mejdd.org"],"crossmark-restriction":false},"short-container-title":["Middle East J Dig Dis"],"published-print":{"date-parts":[[2022,4,30]]},"abstract":"<jats:p>BACKGROUND: Inflammatory cytokines play roles in the pathogenesis of celiac disease. To introduce new diagnostic markers in patients with celiac disease for easy, fast, low cost, and non-invasive diagnosis, we evaluated the peripheral blood expression levels of interleukin-15 (IL-15), interleukin-17A (IL-17A), interleukin23A (IL-23A), granzyme B (GzmB), T-box transcription factor 21 (TBX21), and tumor necrosis factor alpha-induced protein 3 (TNFAIP3) of patients compared with the healthy controls, which were extracted from public databases organized in a protein-protein interaction network, in this group. METHODS: Peripheral blood mononuclear cells were collected from 30 patients with celiac disease and 30 healthy subjects. Total RNA was extracted, and mRNA expression levels of targeted genes were investigated by the quantitative real-time polymerase chain reaction (PCR) method. SPSS software was used for statistical analysis. Receiver operating characteristic (ROC) curve analysis was performed to characterize the diagnostic ability of the studied genes. RESULTS: The expression of IL-15, IL-17A, IL-23A, GzmB, TBX21, and TNFAIP3 genes in peripheral blood mononuclear cells of patients with celiac disease showed a significant increase compared with the control group. Among them, TNFAIP3, IL23A, and GzmB have better resolution and diagnostic value in differentiating patients with celiac disease from healthy controls. CONCLUSION: Our results suggest that TNFAIP3, IL23A, and GzmB could be useful and sensible markers in differentiating patients with celiac disease from healthy controls. However, the diagnostic relevance of other genes recognized by pathway analysis needs to be further investigated. <\/jats:p>","DOI":"10.34172\/mejdd.2022.272","type":"journal-article","created":{"date-parts":[[2022,8,6]],"date-time":"2022-08-06T09:48:10Z","timestamp":1659779290000},"page":"192-199","update-policy":"https:\/\/doi.org\/10.34172\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Introducing New Potential Biomarkers for Celiac Disease among the Genes Extracted from General Databases"],"prefix":"10.34172","volume":"14","author":[{"given":"Ensieh","family":"Khalkhal","sequence":"first","affiliation":[{"name":"Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mostafa","family":"Rezaei-Tavirani","sequence":"additional","affiliation":[{"name":"Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nastaran","family":"Asri","sequence":"additional","affiliation":[{"name":"Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatemeh","family":"Nobakht","sequence":"additional","affiliation":[{"name":"Chemical Injuries Research Center, Systems Biology and Poisoning Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Somayeh","family":"Jahani-Sherafat","sequence":"additional","affiliation":[{"name":"Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Hosain","family":"Haidari","sequence":"additional","affiliation":[{"name":"Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Rostami-Nejad","sequence":"additional","affiliation":[{"name":"Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"20123","published-online":{"date-parts":[[2022,4,30]]},"reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra071600"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.2147\/tacg.s276596"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.34172\/mejdd.2020.187"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2006.11.014"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12191"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2010.01.003"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01374"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.06.018"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/s0016-5085(03)01047-3"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1177\/039463201302600408"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/182549"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12083"},{"key":"R13","doi-asserted-by":"crossref","unstructured":"Mizoguchi A, Andoh A. 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