{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T02:39:42Z","timestamp":1788748782628,"version":"build-2803163510"},"reference-count":20,"publisher":"Central Research Institute for Epidemiology","issue":"2","license":[{"start":{"date-parts":[[2021,5,15]],"date-time":"2021-05-15T00:00:00Z","timestamp":1621036800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Problems of Virology"],"abstract":"<jats:p>Introduction. The pandemic spread of a new coronavirus infection, COVID-19, has caused a global emergency and attracted the attention of public health professionals and the population of all countries. A significant increase in the number of new cases of SARS-CoV-2 infection demonstrates the urgency of finding drugs effective against this pathogen.The aim of this work was to evaluate the in vitro antiviral efficacy of human recombinant alpha-2b interferon (IFN-\u03b12b) against SARS-CoV-2 virus.Material and methods. The experiments had been carried out on Vero Cl008, the continuous line of African green monkey (Chlorocebus sabaeus) kidney cells. The effectiveness of the drugs was assessed by the suppression of viral reproduction in vitro. The biological activity was determined using titration of a virus-containing suspension in a Vero Cl008 cell culture by the formation of negative colonies.Results. The antiviral efficacy of the IFN-\u03b12b-based medications, which have a high safety profile and proven efficacy in the prevention and treatment of influenza and acute respiratory viral infections (ARVI), has been studied against the new pandemic SARS-CoV-2 virus in vitro experiments in Vero C1008 cell culture. IFN-\u03b12b effectively inhibits the reproduction of the virus when applied both 24 hrs before and 2 hrs after infection. In the IFN-\u03b12b concentration range 102\u2013106 IU\/ml a complete suppression of the reproduction of the SARS-CoV-2 virus had been demonstrated.Discussion. IFN-\u03b12b demonstrated in vitro high antiviral activity against SARS-CoV-2. In addition, the substance has a high chemotherapeutic index (1000).Conclusion. Medications for intranasal use based on IFN-\u03b12b have high antiviral activity and are promising drugs for in vivo study in terms of prevention and treatment of COVID-19.<\/jats:p>","DOI":"10.36233\/0507-4088-13","type":"journal-article","created":{"date-parts":[[2021,5,15]],"date-time":"2021-05-15T18:10:13Z","timestamp":1621102213000},"page":"123-128","source":"Crossref","is-referenced-by-count":5,"title":["&lt;i&gt;In vitro&lt;\/i&gt; activity of human recombinant alpha-2b interferon against SARS-CoV-2 virus"],"prefix":"10.36233","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6732-8404","authenticated-orcid":false,"given":"S. Ya.","family":"Loginova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5461-3641","authenticated-orcid":false,"given":"V. N.","family":"Sh\u0441hukina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5175-916X","authenticated-orcid":false,"given":"S. V.","family":"Savenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6742-3919","authenticated-orcid":false,"given":"S. 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Therapeutic options for the 2019 novel coronavirus (2019-nCoV). Nat. Rev. Drug Discov. 2020; 19(3): 149\u201350. https:\/\/doi.org\/10.1038\/d41573-020-00016-0.","DOI":"10.1038\/d41573-020-00016-0"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Lythgoe M.P., Rhodes C.J., Ghataorhe P., Attard M., Wharton J., et al. Why drugs fail in clinical trials in pulmonary arterial hypertension, and strategies to succeed in the future. Pharmacol. Ther. 2016; 164: 195\u2013203. https:\/\/doi.org\/10.1016\/j.pharmthera.2016.04.012.","DOI":"10.1016\/j.pharmthera.2016.04.012"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"Lythgoe M.P., Middleton P. Ongoing clinical trials for the management of the COVID-19 pandemic. Trends Pharmacol. Sci. 2020; 41(6): 363\u201382. https:\/\/doi.org\/10.1016\/j.tips.2020.03.006.","DOI":"10.1016\/j.tips.2020.03.006"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"\u0421\u043e\u043b\u043e\u0433\u0443\u0431 \u0422.\u0412., \u0426\u0432\u0435\u0442\u043a\u043e\u0432 \u0412.\u0412. \u041a\u0430\u0433\u043e\u0446\u0435\u043b \u0432 \u0442\u0435\u0440\u0430\u043f\u0438\u0438 \u0433\u0440\u0438\u043f\u043f\u0430 \u0438 \u043e\u0441\u0442\u0440\u044b\u0445 \u0440\u0435\u0441\u043f\u0438\u0440\u0430\u0442\u043e\u0440\u043d\u044b\u0445 \u0432\u0438\u0440\u0443\u0441\u043d\u044b\u0445 \u0438\u043d\u0444\u0435\u043a\u0446\u0438\u0439: \u0430\u043d\u0430\u043b\u0438\u0437 \u0438 \u0441\u0438\u0441\u0442\u0435\u043c\u0430\u0442\u0438\u0437\u0430\u0446\u0438\u044f \u0434\u0430\u043d\u043d\u044b\u0445 \u043f\u043e \u0440\u0435\u0437\u0443\u043b\u044c\u0442\u0430\u0442\u0430\u043c \u0434\u043e\u043a\u043b\u0438\u043d\u0438\u0447\u0435\u0441\u043a\u0438\u0445 \u0438 \u043a\u043b\u0438\u043d\u0438\u0447\u0435\u0441\u043a\u0438\u0445 \u0438\u0441\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u043d\u0438\u0439. \u0422\u0435\u0440\u0430\u043f\u0435\u0432\u0442\u0438\u0447\u0435\u0441\u043a\u0438\u0439 \u0430\u0440\u0445\u0438\u0432. 2017; 89(8): 113\u20139. https:\/\/doi.org\/10.17116\/terarkh2017898113-119.","DOI":"10.17116\/terarkh2017898113-119"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"\u0415\u0440\u0448\u043e\u0432 \u0424.\u0418., \u041d\u0430\u0440\u043e\u0432\u043b\u044f\u043d\u0441\u043a\u0438\u0439 \u0410.\u041d. \u0422\u0435\u043e\u0440\u0435\u0442\u0438\u0447\u0435\u0441\u043a\u0438\u0435 \u0438 \u043f\u0440\u0438\u043a\u043b\u0430\u0434\u043d\u044b\u0435 \u0430\u0441\u043f\u0435\u043a\u0442\u044b \u0441\u0438\u0441\u0442\u0435\u043c\u044b \u0438\u043d\u0442\u0435\u0440\u0444\u0435\u0440\u043e\u043d\u043e\u0432: \u043a 60-\u043b\u0435\u0442\u0438\u044e \u043e\u0442\u043a\u0440\u044b\u0442\u0438\u044f \u0438\u043d\u0442\u0435\u0440\u0444\u0435\u0440\u043e\u043d\u043e\u0432. \u0412\u043e\u043f\u0440\u043e\u0441\u044b \u0432\u0438\u0440\u0443\u0441\u043e\u043b\u043e\u0433\u0438\u0438. 2018; 63(1): 10\u20138. https:\/\/doi.org\/10.18821\/0507-4088-2018-63-1-10-18.","DOI":"10.18821\/0507-4088-2018-63-1-10-18"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"Sallard E., Lescure F.X., Yazdanpanah Y., Mentre F., Peiffer-Smadja N. Type 1 interferons as a potential treatment against COVID-19. Antiviral Res. 2020; 178: 104791. https:\/\/doi.org\/10.1016\/j.antiviral.2020.104791.","DOI":"10.1016\/j.antiviral.2020.104791"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Kopecky-Bromberg S.A., Martinez-Sobrido L., Frieman M., Baric R.A., Palese P. Severe acute respiratory syndrome coronavirus open reading frame (ORF) 3b, ORF 6, and nucleocapsid proteins function as interferon antagonists. J. Virol. 2007; 81(2): 548\u201357. https:\/\/doi.org\/10.1128\/jvi.01782-06.","DOI":"10.1128\/JVI.01782-06"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Lokugamage K.G., Schindewolf C., Menachery V.D. SARSCoV-2 sensitive to type I interferon pretreatment. bioRxiv. 2020; 03.07.982264. Preprint. https:\/\/doi.org\/10.1101\/2020.03.07.982264 (accessed March 20, 2021).","DOI":"10.1101\/2020.03.07.982264"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Channappanavar R., Fehr A.R., Zheng J., Wohlford-Lenane C., Abrahante J.E., Mack M., et al. IFN-I response timing relative to virus replication determines MERS coronavirus infection outcomes. J. Clin. Invest. 2019; 129(9): 3625\u201339. https:\/\/doi.org\/10.1172\/jci126363.","DOI":"10.1172\/JCI126363"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Siddiqi H.K., Mehra M.R. COVID-19 illness in native and immunosuppressed states: a clinical-therapeutic staging proposal. J. Heart Lung Transplant. 2020; 39(5): 405\u20137. https:\/\/doi.org\/10.1016\/j.healun.2020.03.012.","DOI":"10.1016\/j.healun.2020.03.012"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Zhou F., Yu T., Du R., Fan G., Liu Y., Liu Z., et al. Clinical course and risk factors for mortality of adult inpatients with COVID-19 in Wuhan, China: a retrospective cohort study. Lancet. 2020; 395(10229): 1054\u201362. https:\/\/doi.org\/10.1016\/S0140-6736(20)30566-3.","DOI":"10.1016\/S0140-6736(20)30566-3"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"Zhang W., Zhao Y., Zhang F., Wang Q., Li T., Liu Z., et al. The use of anti-inflammatory drugs in the treatment of people with severe coronavirus disease 2019 (COVID-19): the perspectives of clinical immunologists from China. Clin. Immunol. 2020; 214: 108393. https:\/\/doi.org\/10.1016\/j.clim.2020.108393.","DOI":"10.1016\/j.clim.2020.108393"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"Al-Tawfiq J.A., Momattin H., Dib J., Memish Z.A. Ribavirin and interferon therapy in patients infected with the Middle East respiratory syndrome coronavirus: an observational study. Int. J. Infect. Dis. 2014; 20: 42\u20136. https:\/\/doi.org\/10.1016\/j.ijid.2013.12.003.","DOI":"10.1016\/j.ijid.2013.12.003"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Shalhoub S., Farahat F., Al-Jiffri A., Simhairi R., Shamma O., Siddiqi N., et al. IFN-\u03b12a or IFN-\u03b21a in combination with ribavirin to treat Middle East respiratory syndrome coronavirus pneumonia: a retrospective study. J. Antimicrob. Chemother. 2015; 70(7): 2129\u201332. https:\/\/doi.org\/10.1093\/jac\/dkv085.","DOI":"10.1093\/jac\/dkv085"},{"key":"ref16","unstructured":"\u0421\u044b\u0440\u043e\u043c\u044f\u0442\u043d\u0438\u043a\u043e\u0432\u0430 \u0421.\u0418., \u041f\u0438\u0441\u0446\u043e\u0432 \u041c.\u041d., \u0411\u043e\u0440\u0438\u0441\u0435\u0432\u0438\u0447 \u0421.\u0412., \u0425\u0430\u043c\u0438\u0442\u043e\u0432 \u0420.\u0410., \u041c\u0430\u0440\u043a\u043e\u0432 \u0412.\u0418., \u041c\u0430\u043a\u0441\u0438\u043c\u043e\u0432 \u0412.\u0410. \u0421\u043e\u0441\u0442\u0430\u0432 \u0430\u0433\u0430\u0440\u043e\u0432\u043e\u0433\u043e \u043f\u043e\u043a\u0440\u044b\u0442\u0438\u044f \u0434\u043b\u044f \u0442\u0438\u0442\u0440\u043e\u0432\u0430\u043d\u0438\u044f \u043c\u0435\u0442\u043e\u0434\u043e\u043c \u043d\u0435\u0433\u0430\u0442\u0438\u0432\u043d\u044b\u0445 \u043a\u043e\u043b\u043e\u043d\u0438\u0439 \u043a\u043e\u0440\u043e\u043d\u0430\u0432\u0438\u0440\u0443\u0441\u0430 \u2013 \u0432\u043e\u0437\u0431\u0443\u0434\u0438\u0442\u0435\u043b\u044f \u0442\u044f\u0436\u0451\u043b\u043e\u0433\u043e \u043e\u0441\u0442\u0440\u043e\u0433\u043e \u0440\u0435\u0441\u043f\u0438\u0440\u0430\u0442\u043e\u0440\u043d\u043e\u0433\u043e \u0441\u0438\u043d\u0434\u0440\u043e\u043c\u0430. \u041f\u0430\u0442\u0435\u043d\u0442 \u0420\u0424 \u2116 2325436; 2008. \u041f\u0440\u0438\u043e\u0440\u0438\u0442\u0435\u0442 \u0438\u0437\u043e\u0431\u0440\u0435\u0442\u0435\u043d\u0438\u044f \u043e\u0442 27 \u043d\u043e\u044f\u0431\u0440\u044f 2005 \u0433."},{"key":"ref17","unstructured":"\u0420\u0443\u043a\u043e\u0432\u043e\u0434\u0441\u0442\u0432\u043e \u043f\u043e \u044d\u043a\u0441\u043f\u0435\u0440\u0438\u043c\u0435\u043d\u0442\u0430\u043b\u044c\u043d\u043e\u043c\u0443 (\u0434\u043e\u043a\u043b\u0438\u043d\u0438\u0447\u0435\u0441\u043a\u043e\u043c\u0443) \u0438\u0437\u0443\u0447\u0435\u043d\u0438\u044e \u043d\u043e\u0432\u044b\u0445 \u0444\u0430\u0440\u043c\u0430\u043a\u043e\u043b\u043e\u0433\u0438\u0447\u0435\u0441\u043a\u0438\u0445 \u0432\u0435\u0449\u0435\u0441\u0442\u0432. \u041c\u043e\u0441\u043a\u0432\u0430: \u041c\u0438\u043d\u0437\u0434\u0440\u0430\u0432 \u0420\u0424; 2013."},{"key":"ref18","unstructured":"\u041b\u0430\u0433\u0443\u0442\u043a\u0438\u043d \u041d.\u0410., \u041c\u0438\u0442\u0438\u043d \u041d.\u0418., \u0421\u0442\u0430\u0440\u043e\u0432\u043e\u0439\u0442\u043e\u0432\u0430 \u0412.\u0410. \u041c\u0435\u0442\u043e\u0434\u0438\u0447\u0435\u0441\u043a\u0438\u0435 \u043f\u043e\u0434\u0445\u043e\u0434\u044b \u043a \u043f\u043e\u0438\u0441\u043a\u0443 \u0430\u043d\u0442\u0438\u0432\u0438\u0440\u0443\u0441\u043d\u044b\u0445 \u043f\u0440\u0435\u043f\u0430\u0440\u0430\u0442\u043e\u0432, \u0438\u0445 \u0438\u0441\u043f\u044b\u0442\u0430\u043d\u0438\u0435 \u0438 \u043e\u0446\u0435\u043d\u043a\u0430. \u0412 \u043a\u043d.: \u041b\u043e\u0436\u0430 \u0412.\u041f., \u0418\u043d\u0434\u0443\u043b\u0435\u043d \u041c.\u041a., \u041a\u0430\u043b\u043d\u044b\u043d\u044f \u0412.\u0410., \u041a\u0430\u043d\u0435\u043b\u044c \u041d.\u0410., \u0440\u0435\u0434. \u0412\u0438\u0440\u0443\u0441\u043d\u044b\u0435 \u0438\u043d\u0433\u0438\u0431\u0438\u0442\u043e\u0440\u044b \u0438 \u043c\u0435\u0445\u0430\u043d\u0438\u0437\u043c \u0438\u0445 \u0434\u0435\u0439\u0441\u0442\u0432\u0438\u044f. \u0420\u0438\u0433\u0430: \u0417\u0438\u043d\u0430\u0442\u043d\u0435; 1977."},{"key":"ref19","unstructured":"\u0427\u0438\u0436\u043e\u0432 \u041d.\u041f., \u0415\u0440\u0448\u043e\u0432 \u0424.\u0418., \u0418\u043d\u0434\u0443\u043b\u0435\u043d \u041c.\u041a. \u041e\u0441\u043d\u043e\u0432\u044b \u044d\u043a\u0441\u043f\u0435\u0440\u0438\u043c\u0435\u043d\u0442\u0430\u043b\u044c\u043d\u043e\u0439 \u0445\u0438\u043c\u0438\u043e\u0442\u0435\u0440\u0430\u043f\u0438\u0438 \u0432\u0438\u0440\u0443\u0441\u043d\u044b\u0445 \u0438\u043d\u0444\u0435\u043a\u0446\u0438\u0439. \u0420\u0438\u0433\u0430: \u0417\u0438\u043d\u0430\u0442\u043d\u0435; 1988."},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Shen K.L., Yang Y.H. Diagnosis and treatment of 2019 novel coronavirus infection in children: a pressing issue. World J. Pediatr. 2020; 16(3): 219\u201321. https:\/\/doi.org\/10.1007\/s12519-020-00344-6.","DOI":"10.1007\/s12519-020-00344-6"}],"container-title":["Problems of Virology"],"original-title":[],"link":[{"URL":"https:\/\/virusjour.crie.ru\/jour\/article\/viewFile\/496\/311","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/virusjour.crie.ru\/jour\/article\/viewFile\/496\/311","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,17]],"date-time":"2022-05-17T13:12:40Z","timestamp":1652793160000},"score":1,"resource":{"primary":{"URL":"https:\/\/virusjour.crie.ru\/jour\/article\/view\/496"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,15]]},"references-count":20,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2021,5,15]]}},"URL":"https:\/\/doi.org\/10.36233\/0507-4088-13","relation":{},"ISSN":["2411-2097","0507-4088"],"issn-type":[{"value":"2411-2097","type":"electronic"},{"value":"0507-4088","type":"print"}],"subject":[],"published":{"date-parts":[[2021,5,15]]}}}