{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T03:21:21Z","timestamp":1786418481762,"version":"build-2736575974"},"reference-count":33,"publisher":"Central Research Institute for Epidemiology","issue":"2","license":[{"start":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T00:00:00Z","timestamp":1684368000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["virusjour.crie.ru"],"crossmark-restriction":false},"short-container-title":["Problems of Virology"],"abstract":"<jats:p>Introduction. The COVID-19 pandemic combined with seasonal epidemics of respiratory viral diseases requires targeted antiviral prophylaxis with restorative and immunostimulant drugs. The compounds of natural origin are low-toxic, but active against several viruses at the same time. One of the most famous compounds is Inonotus obliquus aqueous extract. The fruit body of basidial fungus I. obliquus is called Chaga mushroom.\r\nThe aim of the work \u2012 was to study the antiviral activity of I. obliquus aqueous extract against the SARS-CoV-2 virus in vivo.\r\nMaterials and methods. Antiviral activity of I. obliquus aqueous extract sample (#20-17) was analyzed against strain of SARS-CoV-2 Omicron \u0412\u0410.5.2 virus. The experiments were carried out in BALB\/c inbred mice. The SARS-CoV-2 viral load was measured using quantitative real-time PCR combined with reverse transcription. The severity of lung tissue damage was assessed by histological methods.\r\nResults. The peak values of the viral load in murine lung tissues were determined 72 hours after intranasal inoculation at dose of 2,85 lg TCID50. The quantitative real-time PCR testing has shown a significant decrease in the viral load compared to the control group by 4,65 lg copies\/ml and 5,72 lg copies\/ml in the lung tissue and nasal cavity samples, respectively. Histological methods revealed that the decrease in the number and frequency of observed pathomorphological changes in murine lung tissues depended on the introduction of the compound under study.\r\nConclusion. The results obtained indicate the possibility of using basidial fungus Inonotus obliquus aqueous extract as a preventive agent against circulating variants of SARS-CoV-2 virus.<\/jats:p>","DOI":"10.36233\/0507-4088-168","type":"journal-article","created":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T04:09:23Z","timestamp":1684382963000},"page":"152-160","update-policy":"https:\/\/doi.org\/10.36233\/0372-9311-crossmark","source":"Crossref","is-referenced-by-count":2,"title":["Antiviral activity of basidial fungus &lt;i&gt;Inonotus obliquus&lt;\/i&gt; aqueous extract against SARS-C\u043eV-2 virus (Coronaviridae: Betacoronavirus: Sarbecovirus) in vivo in BALB\/c mice model"],"prefix":"10.36233","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1201-8307","authenticated-orcid":false,"given":"Andrey V.","family":"Shipovalov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8251-7040","authenticated-orcid":false,"given":"Gleb A.","family":"Kudrov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7857-6822","authenticated-orcid":false,"given":"Mikhail Yu.","family":"Kartashov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2522-1657","authenticated-orcid":false,"given":"Irina A.","family":"Drachkova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3340-8750","authenticated-orcid":false,"given":"Oleg V.","family":"Pyankov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2028-6099","authenticated-orcid":false,"given":"Vladimir V.","family":"Omigov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6746-8092","authenticated-orcid":false,"given":"Oleg S.","family":"Taranov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4754-5051","authenticated-orcid":false,"given":"Tamara V.","family":"Teplyakova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"22004","published-online":{"date-parts":[[2023,5,18]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Zhu N., Zhang D., Wang W., Li X., Yang B., Song J., et al. 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