{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T06:31:06Z","timestamp":1762929066940,"version":"3.37.3"},"reference-count":25,"publisher":"Central Research Institute for Epidemiology","issue":"2","license":[{"start":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T00:00:00Z","timestamp":1714953600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["virusjour.crie.ru"],"crossmark-restriction":false},"short-container-title":["Problems of Virology"],"abstract":"<jats:p>Introduction. Tick-borne infections are of great importance for many regions of Russia, including Eastern Siberia. This unfavorable epidemiological situation can be characterized not only by the circulation of well-known tick-borne infections, but also by the identification of new pathogens, the role of which remains little or generally unexplored. Multicomponent flavi-like viruses can cause infectious diseases in humans and pose a threat to public health.\r\nThe purpose of the study was the identification and molecular genetic characterization of the Alongshan virus (Flaviviridae, ALSV) isolates, transmitted by ticks in the south of Eastern Siberia.\r\nMaterials and methods. Total 1060 ticks were collected and analyzed from the territory of the Republics of Khakassia, Tuva, Buryatia, Irkutsk Region and Transbaikal Territory (Zabaykalsky Krai) in the spring-summer period 2023. ALSV RNA was detected by RT-PCR followed by nucleotide sequence determination and phylogenetic analysis for each segment of the genome.\r\nResults. The ALSV infection rate in Ixodes persulcatus ticks collected in the Republic of Khakassia was 3.3% (95% CI: 1.4\u20137.5); in Irkutsk Oblast \u2013 1.0% (95% CI: 0.3\u20133.7); in the Republic of Tuva \u2013 0.9% (95% CI: 0.3\u20133.4) and in Transbaikal Krai \u2013 0.7% (95% CI: 0.2\u20133.6). Sequences of all four segments of ALSV genetic variants circulating in I. persulcatus ticks in the south of Eastern Siberia are grouped with sequences found in China and clustered into the Asian subgroup transmitted by taiga ticks. The level of difference in the nucleotide sequences of genome fragments among the identified genetic variants of ALSV ranged from 2 to 3%.\r\nConclusion. The article shows the widespread distribution of ALSV in I. persulcatus ticks in the Republics of Khakassia and Tyva, Irkutsk Oblast and Transbaikal Territory. The obtained data actualize monitoring of changes in the area of distribution of potentially dangerous for humans flavi-like viruses and their vectors.<\/jats:p>","DOI":"10.36233\/0507-4088-223","type":"journal-article","created":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T19:04:48Z","timestamp":1715022288000},"page":"151-161","update-policy":"https:\/\/doi.org\/10.36233\/0372-9311-crossmark","source":"Crossref","is-referenced-by-count":5,"title":["Prevalence and genetic diversity of the &lt;i&gt;Alongshan virus (Flaviviridae)&lt;\/i&gt; circulating in ticks in the south of Eastern Siberia"],"prefix":"10.36233","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7857-6822","authenticated-orcid":false,"given":"Mikhail Yu.","family":"Kartashov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5181-0415","authenticated-orcid":false,"given":"Ekaterina I.","family":"Krivosheina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8148-5242","authenticated-orcid":false,"given":"Valentina Yu.","family":"Kurushina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8491-2363","authenticated-orcid":false,"given":"Alexander B.","family":"Moshkin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4884-7919","authenticated-orcid":false,"given":"Sergey S.","family":"Khankhareev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1622-0553","authenticated-orcid":false,"given":"Choigana R.","family":"Biche-ool","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9507-4199","authenticated-orcid":false,"given":"Oksana N.","family":"Pelevina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6276-2388","authenticated-orcid":false,"given":"Nikita V.","family":"Popov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6892-1125","authenticated-orcid":false,"given":"Olga L.","family":"Bogomazova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1275-171X","authenticated-orcid":false,"given":"Vladimir A.","family":"Ternovoi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"22004","published-online":{"date-parts":[[2024,5,6]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Pierson T.C., Diamond M.S. 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Methods Protoc. 2023; 6(5): 79. https:\/\/doi.org\/10.3390\/mps6050079","DOI":"10.3390\/mps6050079"}],"container-title":["Problems of Virology"],"original-title":[],"link":[{"URL":"https:\/\/virusjour.crie.ru\/jour\/article\/viewFile\/16612\/879","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/virusjour.crie.ru\/jour\/article\/viewFile\/16612\/879","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T19:05:19Z","timestamp":1715022319000},"score":1,"resource":{"primary":{"URL":"https:\/\/virusjour.crie.ru\/jour\/article\/view\/16612"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,6]]},"references-count":25,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,5,6]]}},"URL":"https:\/\/doi.org\/10.36233\/0507-4088-223","relation":{},"ISSN":["2411-2097","0507-4088"],"issn-type":[{"type":"electronic","value":"2411-2097"},{"type":"print","value":"0507-4088"}],"subject":[],"published":{"date-parts":[[2024,5,6]]}}}