{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T13:53:40Z","timestamp":1744206820285,"version":"3.37.3"},"reference-count":4,"publisher":"Central Research Institute for Epidemiology","issue":"3","license":[{"start":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T00:00:00Z","timestamp":1720137600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["virusjour.crie.ru"],"crossmark-restriction":false},"short-container-title":["Problems of Virology"],"abstract":"<jats:p>Introduction. Hemorrhagic fever with renal syndrome (HFRS) is the most common zoonotic human viral disease in the Russian Federation. More than 98% of the HFRS cases are caused by Puumala orthohantavirus (PUU). Effective serological tests are required for laboratory diagnosis of HFRS.\r\nObjective. Construction of an enzyme immunoassay (ELISA) test system for detection of specific antibodies using standard antigen in the form of highly purified inactivated PUU virus as immunosorbent.\r\nMaterials and methods. Preparation of PUU virus antigen, designing the ELISA for detection of specific antibodies, developing parameters of the ELISA system, parallel titration of HFRS patients sera by fluorescent antibody technique (FAT) and the new ELISA.\r\nResults and discussion. For the first time, ELISA based on purified inactivated PUU virus as standard antigen directly absorbed onto immunoplate was developed. Parallel titration of 50 samples from HFRS patients blood sera using FAT and the developed ELISA showed high sensitivity and specificity of this ELISA, with 100% concordance of testing results and significant level of correlation between the titers of specific antibodies in the two assays.\r\nConclusion. The ELISA based on purified inactivated PUU virus as an immunosorbent can be effectively used for HFRS serological diagnosis and for mass seroepidemiological studies.<\/jats:p>","DOI":"10.36233\/0507-4088-230","type":"journal-article","created":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T13:11:01Z","timestamp":1720185061000},"page":"285-289","update-policy":"https:\/\/doi.org\/10.36233\/0372-9311-crossmark","source":"Crossref","is-referenced-by-count":1,"title":["Enzyme immunoassay system for serological diagnosis of hemorrhagic fever with renal syndrome based on inactivated purified Puumala virus (Hantaviridae: &lt;i&gt;Orthohantavirus&lt;\/i&gt;)"],"prefix":"10.36233","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4576-7136","authenticated-orcid":false,"given":"Alexander P.","family":"Ivanov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6656-1682","authenticated-orcid":false,"given":"Tamara K.","family":"Dzagurova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9934-699X","authenticated-orcid":false,"given":"Svetlana S.","family":"Kurashova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2117-597X","authenticated-orcid":false,"given":"Rostislav D.","family":"Teodorovich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4288-6818","authenticated-orcid":false,"given":"Tatyana D.","family":"Klebleeva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6829-1241","authenticated-orcid":false,"given":"Eugeny A.","family":"Tkachenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"22004","published-online":{"date-parts":[[2024,7,5]]},"reference":[{"key":"ref1","unstructured":"Dzagurova T.K., Tkachenko E.A., Petrov V.A. The effectiveness of the use of cultural antigens for serodiagnostics of GLPS using the immunofluorescence method. Voprosy virusologii. 1988; 33(1): 71\u20135. EDN: https:\/\/elibrary.ru\/jfmqvh (in Russian)"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01310902"},{"key":"ref3","unstructured":"Rezapkin G.V., Tkachenko E.A., Ivanov A.P., Bashkirtsev V.N., Dzagurova T.K. Determination of arenavirus antigens and antibodies by solid-phase radioimmunological analysis. Voprosy virusologii. 1981; 26(4): 459\u201363. (in Russian)"},{"key":"ref4","unstructured":"Tkachenko E.A., Ishmukhametov A.A., Dzagurova T.K., Sinyugina A.A., Korotina N.A., Nabatnikov P.A., et al. Manufacturing techniques and methods of control of the inactivated Vero cell-derived vaccine against HFRS has been developed in Russia. Remedium. 2015; (6): 47\u201354. EDN: https:\/\/elibrary.ru\/uabrmj (in Russian)"}],"container-title":["Problems of Virology"],"original-title":[],"link":[{"URL":"https:\/\/virusjour.crie.ru\/jour\/article\/viewFile\/16622\/897","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/virusjour.crie.ru\/jour\/article\/viewFile\/16622\/897","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T14:26:50Z","timestamp":1720189610000},"score":1,"resource":{"primary":{"URL":"https:\/\/virusjour.crie.ru\/jour\/article\/view\/16622"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,5]]},"references-count":4,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,7,5]]}},"URL":"https:\/\/doi.org\/10.36233\/0507-4088-230","relation":{},"ISSN":["2411-2097","0507-4088"],"issn-type":[{"type":"electronic","value":"2411-2097"},{"type":"print","value":"0507-4088"}],"subject":[],"published":{"date-parts":[[2024,7,5]]}}}