{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T04:41:00Z","timestamp":1778474460461,"version":"3.51.4"},"reference-count":26,"publisher":"Central Research Institute for Epidemiology","issue":"6","license":[{"start":{"date-parts":[[2024,12,15]],"date-time":"2024-12-15T00:00:00Z","timestamp":1734220800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["virusjour.crie.ru"],"crossmark-restriction":false},"short-container-title":["Problems of Virology"],"abstract":"<jats:p>Introduction. Varicella-Zoster virus (VZV) is a highly contagious alpha-herpes virus. The diagnosis of chickenpox remains a difficult task especially in cases of breakthrough chickenpox, so the development of reliable laboratory tests is necessary. The simplest and most sensitive serological test for detecting antibodies in human and animal sera is the passive hemagglutination reaction (PHAR).\r\nThe aim. To develop of a simple, highly sensitive and specific serological tests for the detection of antibodies to VZV in human and animal blood sera.\r\nMaterials and methods. Human and animal cell cultures; various strains of VZV; human and animal immune sera; monoclonal antibody to VZV glycoprotein (GP) E. Formalin-treated erythrocytes of sheep, chickens and goats, sensibilised with GP of VZV from a virus-containing supernatant were used for PHAR.\r\nResults. Cell cultures with the maximum cytopathic effect at VZV infection were selected. A simple original method for obtaining virus-specific VZV GPs using lectins has been developed. Purified GPs were obtained by their elution from sheep erythrocytes after adsorption. The activity of VZV GP was confirmed in PHAR by an antibody diagnostic assay using formalin-treated sheep erythrocytes sensibilised using monoclonal antibodies to GP E of the \u201cvOka\u201d VZV strain (USA). Using GPs from different VZV strains, PHAR test and GP-based enzyme-linked immunosorbent assay (gpELISA) have been developed to detect antibodies in human and animal immune sera. These tests have high sensitivity, specificity and lack of cross-reactivity.\r\nConclusion. A highly specific, sensitive and reproducible tests for the detection of antibodies to VZV have been developed.<\/jats:p>","DOI":"10.36233\/0507-4088-259","type":"journal-article","created":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T19:52:34Z","timestamp":1736970754000},"page":"489-499","update-policy":"https:\/\/doi.org\/10.36233\/0372-9311-crossmark","source":"Crossref","is-referenced-by-count":1,"title":["A simple, highly sensitive and specific serological test for the detection of antibodies to Varicella-zoster virus (&lt;i&gt;Varicellovirus humanalpha3&lt;\/i&gt;)"],"prefix":"10.36233","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8204-4899","authenticated-orcid":false,"given":"Firaya G.","family":"Nagieva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3369-8869","authenticated-orcid":false,"given":"Elena P","family":"Barkova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2169-9610","authenticated-orcid":false,"given":"Olga S.","family":"Kharchenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3561-8295","authenticated-orcid":false,"given":"Alexander V.","family":"Sidorov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9887-4061","authenticated-orcid":false,"given":"Galina I.","family":"Alatortseva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5803-6263","authenticated-orcid":false,"given":"Bogdan S.","family":"Cherepovich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3226-5989","authenticated-orcid":false,"given":"Yulia N.","family":"Tarakanova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0821-5553","authenticated-orcid":false,"given":"Olga A.","family":"Trubacheva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5682-4581","authenticated-orcid":false,"given":"Evgeny A.","family":"Pashkov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4212-5093","authenticated-orcid":false,"given":"Artem A.","family":"Rtishchev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1757-8389","authenticated-orcid":false,"given":"Oksana A.","family":"Svitich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5808-2246","authenticated-orcid":false,"given":"Vitaly V.","family":"Zverev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"22004","published-online":{"date-parts":[[2024,12,15]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Gershon A.A., Breuer J., Cohen J.I., Cohrs R.J., Gershon M.D., Gilden D., et al. Varicella zoster virus infection. Nat. Rev. Dis. Primers. 2015; 1: 15016. https:\/\/doi.org\/10.1038\/nrdp.2015.16","DOI":"10.1038\/nrdp.2015.16"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Arvin A.M., Moffat J.F., Abendroth A., Oliver S.L., eds. Varicella-zoster Virus. Genetics, Pathogenesis and Immunity. 6th ed. Cham: Springer; 2023. https:\/\/doi.org\/10.1007\/978-3-031-15305-1","DOI":"10.1007\/978-3-031-15305-1"},{"key":"ref3","unstructured":"Varicella and herpes zoster vaccines: WHO position paper, June 2014. Wkly Epidemiol Rec. 2014; 89(25): 265\u201387."},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"Heininger U., Seward J.F. Varicella. Lancet. 2006; 368(9544): 1365\u201376. https:\/\/doi.org\/10.1016\/S0140-6736(06)69561-5","DOI":"10.1016\/S0140-6736(06)69561-5"},{"key":"ref5","unstructured":"Harpaz R., Ortega-Sanchez I.R., Seward J.F. Prevention of herpes zoster: recommendations of the Advisory Committee on Immunization Practices (ACIP). MMWR Recomm. Rep. 2008; 57(RR-5): 1\u201330; quiz CE2-4."},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"Cohen J.I. Clinical practice: Herpes zoster. N. Engl. J. Med. 2013; 369(3): 255\u201363. https:\/\/doi.org\/10.1056\/NEJMcp1302674","DOI":"10.1056\/NEJMcp1302674"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"Shin D., Shin Y., Kim E., Nam H., Nan H., Lee J. Immunological characteristics of MAV\/06 strain of varicella-zoster virus vaccine in an animal model. BMC Immunol. 2022; 23(1): 27. https:\/\/doi.org\/10.1186\/s12865-022-00503-6","DOI":"10.1186\/s12865-022-00503-6"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Higashimoto Y., Hattori F., Kawamura Y., Kozawa K., Hamano A., Kato M., et al. Analysis of the reliability of rapid diagnostic tests for varicella, including breakthrough cases. J. Med. Virol. 2023; 95(2): e28569. https:\/\/doi.org\/10.1002\/jmv.28569","DOI":"10.1002\/jmv.28569"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Pan D., Wang W., Cheng T. Current methods for the detection of antibodies of varicella-zoster virus: a review. Microorganisms. 2023; 11(2): 519. https:\/\/doi.org\/10.3390\/microorganisms11020519","DOI":"10.3390\/microorganisms11020519"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Otani N., Shima M., Tanimura S., Ueda T., Ichiki K., Nakajima K., et al. Sensitivity and specificity of different antibody tests for detecting varicella-zoster virus. J. Infect. Chemother. 2020; 26(12): 1283\u20137. https:\/\/doi.org\/10.1016\/j.jiac.2020.07.012","DOI":"10.1016\/j.jiac.2020.07.012"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Mo C., Lee J., Sommer M., Grose C., Arvin A.M. The requirement of varicella zoster virus glycoprotein E (gE) for viral replication and effects of glycoprotein I on gE in melanoma cells. Virology. 2002; 304(2): 176\u201386. https:\/\/doi.org\/10.1006\/viro.2002.1556","DOI":"10.1006\/viro.2002.1556"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Berarducci B., Rajamani J., Reichelt M., Sommer M., Zerboni L., Arvin A.M. Deletion of the first cysteine-rich region of the varicella-zoster virus glycoprotein E ectodomain abolishes the gE and gI interaction and differentially affects cell-cell spread and viral entry. J. Virol. 2009; 83(1): 228\u201340. https:\/\/doi.org\/10.1128\/JVI.00913-08","DOI":"10.1128\/JVI.00913-08"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"Hwang J.Y., Kim Y., Lee K.M., Shin O.S., Gim J.A., Shin Y., et al. Cross-reactive humoral immunity of clade 2 Oka and MAV\/06 strain-based varicella vaccines against different clades of varicella-zoster virus. Hum. Vaccin. Immunother. 2023; 19(1): 2210961. https:\/\/doi.org\/10.1080\/21645515.2023.2210961","DOI":"10.1080\/21645515.2023.2210961"},{"key":"ref14","unstructured":"Marin M., G\u00fcris D., Chaves S.S., Schmid S., Seward J.F. Prevention of varicella: recommendations of the Advisory Committee on Immunization Practices (ACIP). MMWR Recomm. Rep. 2007; 56(RR-4): 1\u201340."},{"key":"ref15","unstructured":"\u0421enters for Disease Control and Prevention. Chickenpox (Varicella). Available at: https:\/\/cdc.gov\/chickenpox\/hcp\/index.html"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"Lafreniere M.A., Badr E., Beattie J., Macri J., Khan W.I. Performance evaluation system of the Bio\u2013Rad Bioplex 2200 multiplex system in the detection of measles, mumps, rubella, and varicella-zoster antibodies. J. Clin. Virol. Plus. 2023; 3(1): 100131. https:\/\/doi.org\/10.1016\/j.jcvp.2022.100131and","DOI":"10.1016\/j.jcvp.2022.100131"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Coates S.R., Madsen R.D., Rippe D.F. New passive hemagglutination assay kit that uses hemagglutinin-sensitized erythrocytes for detection of rubella antibodies. J. Clin. Microbiol. 1982; 16(6): 1117\u201322. https:\/\/doi.org\/10.1128\/jcm.16.6.1117-1122.1982","DOI":"10.1128\/jcm.16.6.1117-1122.1982"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"Kim K.S., Sapienza V., Chen C.M. Confirmation of human cytomegalovirus by reverse passive hemagglutination with monoclonal antibodies reactive to the major glycosylated peptide (GP-66). J. Clin. Microbiol. 1986; 24(3): 474\u20137. https:\/\/doi.org\/10.1128\/jcm.24.3.474-477.1986","DOI":"10.1128\/jcm.24.3.474-477.1986"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Maduike C.O., Ezeibe A.A., Anene N.I., Amechi B., Eze J.I., Animoke P.C. Direct passive hemagglutination test for rapid quantification of plasma load of the human immunodeficiency virus. Sci. Res. 2013; 5(9): 1351\u20134. http:\/\/dx.doi.org\/10.4236\/health.2013.59183","DOI":"10.4236\/health.2013.59183"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Wasmuth E.H., Miller W.J. Sensitive enzyme-linked immunosorbent assay for antibody to varicella-zoster virus using purified VZV glycoprotein antigen. J. Med. Virol. 1990; 32(3): 189\u201393. https:\/\/doi.org\/10.1002\/jmv.1890320310","DOI":"10.1002\/jmv.1890320310"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"Kino Y., Minamishima Y. Passive hemagglutination assays for the detection of antibodies to herpes viruses. Microbiol. Immunol. 1993; 37(5): 365\u20138. https:\/\/doi.org\/10.1111\/j.1348-0421.1993.tb03223.x","DOI":"10.1111\/j.1348-0421.1993.tb03223.x"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"Weinbach R. Die Verwendbarkeit formolbehandelter Erythocyten als Antigtntrager in der Haemagglutination. Schweiz. Z. Pathol. Bakteriol. 1958; 21(6): 1043\u201352. https:\/\/doi.org\/10.1159\/000160565 (in German)","DOI":"10.1159\/000160565"},{"key":"ref23","unstructured":"Frigo N.V., Komarova V.D., Obryadina A.P., Burkov A.N. Comparative results of enzyme immunoassay, reactions of passive hemagglutination and microreaction in serodiagnostics of syphilis. Vestnik dermatologii i venerologii. 2000; (4): 4\u201336. (in Russian)"},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"Mendelson E., Aboudy Y., Smetana Z., Tepperberg M., Grossman Z. Laboratory assessment and diagnosis of congenital viral infections: Rubella, cytomegalovirus (CMV), varicella-zoster virus (VZV), herpes simplex virus (HSV), parvovirus B19 and human immunodeficiency virus (HIV). Reprod. Toxicol. 2006; 21(4): 350\u201382. https:\/\/doi.org\/10.1016\/j.reprotox.2006.02.001","DOI":"10.1016\/j.reprotox.2006.02.001"},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"Nagieva F.G., Barkova E.P., Lisakov A.N., Sidorov A.V., Zverev V.V., Osokina O.V., et al. Practical aspects on identification, cultivation and characteristics of varicella-zoster virus isolates. Infektsiya i immunitet. 2020; 10(2): 387\u201396. https:\/\/doi.org\/10.15789\/2220-7619-PAO-1211 https:\/\/elibrary.ru\/ptnvte (in Russian)","DOI":"10.15789\/2220-7619-PAO-1211"},{"key":"ref26","unstructured":"Shubladze A.K., Gaidamovich S.Ya. Short Course of Practical Virology [Kratkii kurs prakticheskoi virusologii]. Moscow: Medgiz; 1954. (in Russian)"}],"container-title":["Problems of Virology"],"original-title":[],"link":[{"URL":"https:\/\/virusjour.crie.ru\/jour\/article\/viewFile\/16675\/940","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/virusjour.crie.ru\/jour\/article\/viewFile\/16675\/941","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/virusjour.crie.ru\/jour\/article\/viewFile\/16675\/941","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T19:52:52Z","timestamp":1736970772000},"score":1,"resource":{"primary":{"URL":"https:\/\/virusjour.crie.ru\/jour\/article\/view\/16675"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,15]]},"references-count":26,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,12,15]]}},"URL":"https:\/\/doi.org\/10.36233\/0507-4088-259","relation":{},"ISSN":["2411-2097","0507-4088"],"issn-type":[{"value":"2411-2097","type":"electronic"},{"value":"0507-4088","type":"print"}],"subject":[],"published":{"date-parts":[[2024,12,15]]}}}