{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T17:24:52Z","timestamp":1764350692275,"version":"3.37.3"},"reference-count":31,"publisher":"Central Research Institute for Epidemiology","issue":"4","license":[{"start":{"date-parts":[[2021,9,18]],"date-time":"2021-09-18T00:00:00Z","timestamp":1631923200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Problems of Virology"],"abstract":"<jats:p>Introduction. Tick-borne encephalitis virus (TBEV) is medically most important representative of the same-name serogroup of genus Flavivirus (Flaviviridae). In the view of various researchers there are 3 to 5 TBEV subtypes, of them siberian being the most prevalent. The aim of the work is to compare the biological properties and to reveal phylogenetic relationships of large group of modern (2006\u20132019) TBEV isolates of siberian subtype from natural foci in southern East Siberia.Material and methods. Ixodid ticks (Ixodidae) and small mammals (Mammalia) from tick-borne encephalitis (TBE) natural foci in Irkutsk Region, Republic of Buryatia and Republic of Tuva, as well as specimens from TBE patients, were examined for TBEV markers using enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR). Virus was isolated from suspensions with positive result, and its pathogenicity for white mice (Mus) (WM) was studied by different inoculation ways. Analysis of the nucleotide sequences of E gene was performed for isolates at 1st passage. Phylogenetic tree was constructed using MEGA X program.Results. The phylogenetic analysis has shown that TBEV of siberian subtype that circulates in natural foci of the studied territory belong to two genetic lines. These lines are \u00abVasilchenko\u00bb and \u00abZausaev\u00bb with a strong predominance of the first. The differences in biological properties between the two groups of strains have been demonstrated. Most of the strains from both groups showed high virulence for WM both after intracerebral and subcutaneous inoculation. Only four strains demonstrated the reduced ability to overcome the blood-brain barrier. However, the analysis of the E protein coding sequences revealed evident correlation between phylogenetic clustering and geographical origin of isolates, but not with TBE host or pathogenicity for WM.Conclusion. Further search for TBE genome regions associated with pathogenicity require the analysis of complete genome sequences of representative group of strains with different biological properties.<\/jats:p>","DOI":"10.36233\/0507-4088-55","type":"journal-article","created":{"date-parts":[[2021,9,18]],"date-time":"2021-09-18T19:53:20Z","timestamp":1631994800000},"page":"310-321","source":"Crossref","is-referenced-by-count":2,"title":["Biological properties and phylogenetic relationships of tick-borne encephalitis virus (Flaviviridae, Flavivirus) isolates of siberian subtype isolated in the south of East siberia in modern period"],"prefix":"10.36233","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5133-0323","authenticated-orcid":false,"given":"O. V.","family":"Mel\u2019nikova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3690-3992","authenticated-orcid":false,"given":"R. V.","family":"Adel\u2019shin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8772-5842","authenticated-orcid":false,"given":"K. V.","family":"Lopatovskaya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4501-146X","authenticated-orcid":false,"given":"Yu. T.","family":"Trushina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9994-0371","authenticated-orcid":false,"given":"N. V.","family":"Yakovchits","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6612-479X","authenticated-orcid":false,"given":"E. I.","family":"Andaev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"22004","published-online":{"date-parts":[[2021,9,18]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"R\u016f\u017eek D., Yoshii K., Bloom M.E., Gould E.A. Virology. Chapter 2a. In: Dobler G., Erber W., Br\u00f6ker M., Schmitt H.J., eds. The TBE Book. Singapore: Global Health Press; 2019. https:\/\/doi.org\/10.33442\/978-981-14-0914-1_2a","DOI":"10.33442\/978-981-14-0914-1_2a"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Demina T.V., Dzhioev Yu.P., Verkhozina M.M., Kozlova I.V., Tkachev S.E., Plyusnin A., et al. Genotyping and characterization of the geographical distribution of tick-borne encephalitis virus variants with a set of molecular probes. J. Med. Virol. 2010; 82(6): 965\u201376. https:\/\/doi.org\/10.1002\/jmv.21765","DOI":"10.1002\/jmv.21765"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Kovalev S.Y., Mukhacheva T.A. Reconsidering the classification of tick-borne encephalitis virus within the Siberian subtype gives new insights into its evolutionary history. Infect. Genet. Evol. 2017; 55: 159\u201365. https:\/\/doi.org\/10.1016\/j.meegid.2017.09.014","DOI":"10.1016\/j.meegid.2017.09.014"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"Adelshin R.V., Sidorova E.A., Bondaryuk A.N., Trukhina A.G., Sherbakov D.Y., White III R.A., et al. \u201c886-84-like\u201d tick-borne encephalitis virus strains: Intraspecific status elucidated by comparative genomics. Ticks Tick Borne Dis. 2019; 10(5): 1168\u201372. https:\/\/doi.org\/10.1016\/j.ttbdis.2019.06.006","DOI":"10.1016\/j.ttbdis.2019.06.006"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"Dai X., Shang G., Lu S., Yang J., Xu J. A new subtype of eastern tick-borne encephalitis virus discovered in Qinghai-Tibet Plateau, China. Emerg. Microbes. Infect. 2018; 7(1): 74. https:\/\/doi.org\/10.1038\/s41426-018-0081-6","DOI":"10.1038\/s41426-018-0081-6"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"Tkachev S.E., Chicherina G.S., Golovljova I., Belokopytova P.S., Tikunov A.Y., Zadora O.V., et al. New genetic lineage within the Siberian subtype of tick-borne encephalitis virus found in Western Siberia, Russia. Infect. Genet. Evol. 2017; 56: 36\u201343. https:\/\/doi.org\/10.1016\/j.meegid.2017.10.020","DOI":"10.1016\/j.meegid.2017.10.020"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"Tkachev S.E., Babkin I.V., Chicherina G.S., Kozlova I.V., Verkhozina M.M., Demina T.V., et al. Genetic diversity and geographical distribution of the Siberian subtype of the tick-borne encephalitis virus. 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Virol. 2003; 77(1): 25\u201336. https:\/\/doi.org\/10.1128\/jvi.77.1.25-36.2003","DOI":"10.1128\/JVI.77.1.25-36.2003"},{"key":"ref12","unstructured":"\u041a\u043e\u043d\u044c\u043a\u043e\u0432\u0430-\u0420\u0435\u0439\u0434\u043c\u0430\u043d \u0410.\u0411., \u0417\u043b\u043e\u0431\u0438\u043d \u0412.\u0418. \u041f\u0430\u0442\u043e\u043c\u043e\u0440\u0444\u043e\u0437 \u043a\u043b\u0435\u0449\u0435\u0432\u043e\u0433\u043e \u044d\u043d\u0446\u0435\u0444\u0430\u043b\u0438\u0442\u0430 \u043d\u0430 \u042e\u0436\u043d\u043e\u043c \u0423\u0440\u0430\u043b\u0435: \u0441\u043e\u0432\u0440\u0435\u043c\u0435\u043d\u043d\u043e\u0435 \u0441\u043e\u0441\u0442\u043e\u044f\u043d\u0438\u0435 \u043f\u0440\u043e\u0431\u043b\u0435\u043c\u044b. \u0418\u043d\u0444\u0435\u043a\u0446\u0438\u043e\u043d\u043d\u044b\u0435 \u0431\u043e\u043b\u0435\u0437\u043d\u0438. 2016; 14(S1): 141."},{"key":"ref13","unstructured":"\u041f\u043e\u0433\u043e\u0434\u0438\u043d\u0430 \u0412.\u0412., \u041a\u0430\u0440\u0430\u043d\u044c \u041b.\u0421., \u041a\u043e\u043b\u044f\u0441\u043d\u0438\u043a\u043e\u0432\u0430 \u041d.\u041c., \u041b\u0435\u0432\u0438\u043d\u0430 \u041b.\u0421., \u041c\u0430\u043b\u0435\u043d\u043a\u043e \u0413.\u0412., \u0413\u0430\u043c\u043e\u0432\u0430 \u0415.\u0413., \u0438 \u0434\u0440. \u042d\u0432\u043e\u043b\u044e\u0446\u0438\u044f \u043a\u043b\u0435\u0449\u0435\u0432\u043e\u0433\u043e \u044d\u043d\u0446\u0435\u0444\u0430\u043b\u0438\u0442\u0430 \u0438 \u043f\u0440\u043e\u0431\u043b\u0435\u043c\u0430 \u044d\u0432\u043e\u043b\u044e\u0446\u0438\u0438 \u0432\u043e\u0437\u0431\u0443\u0434\u0438\u0442\u0435\u043b\u044f. \u0412\u043e\u043f\u0440\u043e\u0441\u044b \u0432\u0438\u0440\u0443\u0441\u043e\u043b\u043e\u0433\u0438\u0438. 2007; 52(5): 16\u201321."},{"key":"ref14","unstructured":"\u041a\u043e\u0437\u043b\u043e\u0432\u0430 \u0418.\u0412., \u0412\u0435\u0440\u0445\u043e\u0437\u0438\u043d\u0430 \u041c.\u041c., \u0414\u043e\u0440\u043e\u0449\u0435\u043d\u043a\u043e \u0415.\u041a., \u041b\u0438\u0441\u0430\u043a \u041e.\u0412., \u041a\u0430\u0440\u0430\u043d\u044c \u041b.\u0421., \u0422\u043a\u0430\u0447\u0435\u0432 \u0421.\u0415., \u0438 \u0434\u0440. \u0420\u0435\u0437\u0443\u043b\u044c\u0442\u0430\u0442\u044b \u0433\u0435\u043d\u043e\u0442\u0438\u043f\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f \u0448\u0442\u0430\u043c\u043c\u043e\u0432 \u0438 \u0438\u0437\u043e\u043b\u044f\u0442\u043e\u0432 \u0420\u041d\u041a \u0432\u0438\u0440\u0443\u0441\u0430 \u043a\u043b\u0435\u0449\u0435\u0432\u043e\u0433\u043e \u044d\u043d\u0446\u0435\u0444\u0430\u043b\u0438\u0442\u0430, \u0432\u044b\u0434\u0435\u043b\u0435\u043d\u043d\u044b\u0445 \u043e\u0442 \u0431\u043e\u043b\u044c\u043d\u044b\u0445 \u043b\u044e\u0434\u0435\u0439 \u0432 \u0418\u0440\u043a\u0443\u0442\u0441\u043a\u043e\u0439 \u043e\u0431\u043b\u0430\u0441\u0442\u0438 \u0438 \u0420\u0435\u0441\u043f\u0443\u0431\u043b\u0438\u043a\u0435 \u0411\u0443\u0440\u044f\u0442\u0438\u044f. \u0412 \u043a\u043d.: \u041f\u043e\u043a\u0440\u043e\u0432\u0441\u043a\u0438\u0439 \u0412.\u0418., \u0440\u0435\u0434. \u041c\u043e\u043b\u0435\u043a\u0443\u043b\u044f\u0440\u043d\u0430\u044f \u0434\u0438\u0430\u0433\u043d\u043e\u0441\u0442\u0438\u043a\u0430. \u0422\u043e\u043c 1. \u041c.: \u041c\u0411\u0410; 2014; (1): 520\u20131."},{"key":"ref15","unstructured":"\u041c\u0435\u0439\u0445\u0438 \u0411., \u0440\u0435\u0434. \u0412\u0438\u0440\u0443\u0441\u043e\u043b\u043e\u0433\u0438\u044f. \u041c\u0435\u0442\u043e\u0434\u044b. \u041f\u0435\u0440. \u0441 \u0430\u043d\u0433\u043b. \u041c\u043e\u0441\u043a\u0432\u0430: \u041c\u0438\u0440; 1988."},{"key":"ref16","unstructured":"\u042f\u043a\u0438\u043c\u0435\u043d\u043a\u043e \u0412.\u0412., \u0414\u0440\u043e\u043a\u0438\u043d \u0414.\u0410., \u041a\u0430\u043b\u043c\u0438\u043d \u041e.\u0411., \u0411\u043e\u0433\u0434\u0430\u043d\u043e\u0432 \u0418.\u0418., \u0418\u0432\u0430\u043d\u043e\u0432 \u0414.\u0418. \u041a \u0432\u043e\u043f\u0440\u043e\u0441\u0443 \u043e \u0432\u043b\u0438\u044f\u043d\u0438\u0438 host-\u044d\u0444\u0444\u0435\u043a\u0442\u0430 \u043d\u0430 \u0448\u0442\u0430\u043c\u043c\u043e\u0432\u0443\u044e \u0438\u0437\u043c\u0435\u043d\u0447\u0438\u0432\u043e\u0441\u0442\u044c \u0432\u0438\u0440\u0443\u0441\u0430 \u043a\u043b\u0435\u0449\u0435\u0432\u043e\u0433\u043e \u044d\u043d\u0446\u0435\u0444\u0430\u043b\u0438\u0442\u0430. \u0412\u043e\u043f\u0440\u043e\u0441\u044b \u0432\u0438\u0440\u0443\u0441\u043e\u043b\u043e\u0433\u0438\u0438. 1996; 41(3): 112\u20137."},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Adelshin R.V., Melnikova O.V., Karan L.S., Andaev E.I., Balakhonov S.V. 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