{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T13:19:30Z","timestamp":1774963170324,"version":"3.50.1"},"reference-count":0,"publisher":"Doc Design and Informatics Co. Ltd.","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Objective: This study aimed to develop a sensitive and reliable method for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in various environmental samples using nano\u2013liquid chromatography\u2013tandem mass spectrometry parallel reaction monitoring (nanoLC-MS\/MS PRM), targeting sequences of the viral spike (S) and nucleocapsid (N) proteins.\n\nMaterials and Methods: A synthetic peptide screening approach was employed to systematically evaluate candidate sequences as surrogates for protein quantification. The process began with the digestion of purified SARS-CoV-2 proteins. The candidate sequences were then refined to a subset of target peptides, and stable isotope-labeled heavy peptides were used as standards in a PRM workflow. The detection limits were determined using the nanoLC-MS\/MS PRM method.\n\nResults: The developed method achieved limits of detection in the 1 fmol\/\u00b5L (2\u20138 \u00d7 10-15 viral copies\/L) range on-column. This demonstrates high sensitivity for detecting SARS-CoV-2 in environmental samples using the PRM method.\n\nConclusion: The study successfully developed a sensitive and reliable peptide probe-based approach for detecting SARS-CoV-2 in various environmental samples. The use of the PRM method targeting the S and N proteins of SARS-CoV-2 provides a robust approach for virus detection, particularly given the potential persistence of the virus on surfaces.\n\nKeywords: SARS-CoV-2, spike protein, nucleocapsid protein, nanoLC-MS\/MS PRM<\/jats:p>","DOI":"10.36519\/idcm.2026.770","type":"journal-article","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T12:30:07Z","timestamp":1774960207000},"page":"165","source":"Crossref","is-referenced-by-count":0,"title":["Peptide Probe-Based NanoLC-MS\/MS PRM for Ultrasensitive Detection of SARS-CoV-2 Proteins in Environmental Samples"],"prefix":"10.36519","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5598-1016","authenticated-orcid":false,"given":"Jingping","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2688-5383","authenticated-orcid":false,"given":"Dan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0440-323X","authenticated-orcid":false,"given":"Xiyuan","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0753-6505","authenticated-orcid":false,"given":"Na","family":"Meiri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4653-9856","authenticated-orcid":false,"given":"Dezhi","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"22401","published-online":{"date-parts":[[2026,3,30]]},"container-title":["Infectious Diseases and Clinical Microbiology"],"original-title":[],"deposited":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T12:30:07Z","timestamp":1774960207000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.idcmjournal.org\/sensitive-detection-of-sars-cov-2-in-environment-by-prm"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,30]]},"references-count":0,"URL":"https:\/\/doi.org\/10.36519\/idcm.2026.770","relation":{},"ISSN":["2667-646X"],"issn-type":[{"value":"2667-646X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,30]]}}}