{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:48:39Z","timestamp":1791917319768,"version":"4.3.4"},"reference-count":35,"publisher":"Sociedade Brasileira de Cardiologia","issue":"8","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arq. Bras. Cardiol."],"abstract":"<jats:p>Resumo  Fundamento  A disfun\u00e7\u00e3o mitocondrial \u00e9 um fator chave na insufici\u00eancia card\u00edaca, o que refor\u00e7a a necessidade de terapias que promovam a biog\u00eanese e a fun\u00e7\u00e3o mitocondrial. O polissacar\u00eddeo de Astragalus (APS), um composto bioativo extra\u00eddo da Astragalus membranaceus, demonstrou propriedades cardioprotetoras, mas seus efeitos diretos na homeostase mitocondrial em cardiomi\u00f3citos ainda n\u00e3o est\u00e3o claros.  Objetivos  Este estudo teve como objetivo investigar os efeitos do APS na fun\u00e7\u00e3o mitocondrial e na biog\u00eanese em cardiomi\u00f3citos HL-1 e elucidar o envolvimento da via de sinaliza\u00e7\u00e3o da prote\u00edna quinase ativada por AMP (AMPK)\/coativador 1-alfa do receptor gama ativado por proliferador de peroxissoma (PGC-1\u03b1).  M\u00e9todos  Cardiomi\u00f3citos HL-1 foram tratados com concentra\u00e7\u00f5es n\u00e3o t\u00f3xicas de APS (1,25-2,5 g\/L) por 48 horas. A respira\u00e7\u00e3o mitocondrial foi avaliada por meio do analisador Seahorse XF, os n\u00edveis de adenosina trifosfato (ATP) foram quantificados e a atividade do Complexo IV foi mensurada. A biog\u00eanese mitocondrial foi avaliada pela raz\u00e3o DNA mitocondrial (mtDNA)\/DNA nuclear (nDNA), n\u00edveis de citocromo B e massa mitocondrial. Prote\u00ednas e genes-chave, incluindo PGC-1\u03b1, fator respirat\u00f3rio nuclear 2 (NRF2), fator de transcri\u00e7\u00e3o mitocondrial A (TFAM), prote\u00edna desacopladora 2 (UCP2) e AMPK fosforilada (p-AMPK), foram analisados por Western blot e qPCR. O inibidor de AMPK, Composto C, foi utilizado para confirmar o papel da via.  Resultados  O tratamento com APS aumentou significativamente a respira\u00e7\u00e3o mitocondrial, a produ\u00e7\u00e3o de ATP e a atividade do Complexo IV. Promoveu a biog\u00eanese mitocondrial, evidenciada pelo aumento da raz\u00e3o mtDNA\/nDNA e da massa mitocondrial. O APS aumentou a express\u00e3o de PGC-1\u03b1, NRF2, TFAM e UCP2, e ativou a fosforila\u00e7\u00e3o da AMPK. Todos esses efeitos ben\u00e9ficos foram abolidos pela inibi\u00e7\u00e3o da AMPK com o Composto C.  Conclus\u00f5es  O APS melhora a biog\u00eanese e a fun\u00e7\u00e3o mitocondrial em cardiomi\u00f3citos atrav\u00e9s da ativa\u00e7\u00e3o da via de sinaliza\u00e7\u00e3o AMPK\/PGC-1\u03b1, identificando-o como um potencial agente terap\u00eautico para doen\u00e7as card\u00edacas associadas \u00e0 disfun\u00e7\u00e3o mitocondrial.<\/jats:p>","DOI":"10.36660\/abc.20250854","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:29:37Z","timestamp":1791916177000},"source":"Crossref","is-referenced-by-count":0,"title":["O Polissacar\u00eddeo de Astragalus Melhora a Biog\u00eanese e a Fun\u00e7\u00e3o Mitocondrial em Cardiomi\u00f3citos atrav\u00e9s da Ativa\u00e7\u00e3o da AMPK"],"prefix":"10.36660","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8475-9447","authenticated-orcid":false,"given":"Chengbin","family":"Wang","sequence":"first","affiliation":[{"name":"Guizhou University of Traditional Chinese Medicine","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7772-5200","authenticated-orcid":false,"given":"Shuhan","family":"Lv","sequence":"additional","affiliation":[{"name":"Guizhou University of Traditional Chinese Medicine","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5613-7033","authenticated-orcid":false,"given":"Xingde","family":"Liu","sequence":"additional","affiliation":[{"name":"Guizhou University of Traditional Chinese Medicine","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"22636","published-online":{"date-parts":[[2026]]},"reference":[{"issue":"12","key":"ref1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s12276-019-0355-7","article-title":"Mitochondrial Dysfunction and Oxidative Stress in Heart Disease","volume":"51","author":"Peoples JN","year":"2019","journal-title":"Exp Mol Med"},{"issue":"6","key":"ref2","doi-asserted-by":"crossref","DOI":"10.3390\/biology9060137","article-title":"The Role of Mitochondria in Cardiovascular Diseases","volume":"9","author":"Poznyak AV","year":"2020","journal-title":"Biology"},{"issue":"5","key":"ref3","article-title":"Mitochondrial Dysfunction in Cardiovascular Diseases","volume":"26","author":"Yang HM","year":"2025","journal-title":"Int J Mol Sci"},{"issue":"11","key":"ref4","doi-asserted-by":"crossref","DOI":"10.3390\/antiox12111941","article-title":"Status of Mitochondrial Oxidative Phosphorylation during the Development of Heart Failure","volume":"12","author":"Bhullar SK","year":"2023","journal-title":"Antioxidants"},{"key":"ref5","article-title":"Oxidative Stress and Mitochondrial Impairment: Key Drivers in Neurodegenerative Disorders","volume":"104","author":"Wen P","year":"2025","journal-title":"Ageing Res Rev"},{"key":"ref6","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2021.669379","article-title":"The Mitochondrial Response to DNA Damage","volume":"9","author":"Rong Z","year":"2021","journal-title":"Front Cell Dev Biol"},{"issue":"1","key":"ref7","article-title":"Mitochondrial Diseases: from Molecular Mechanisms to Therapeutic Advances","volume":"10","author":"Wen H","year":"2025","journal-title":"Signal Transduct Target Ther"},{"issue":"7","key":"ref8","doi-asserted-by":"crossref","DOI":"10.3390\/ijms24076128","article-title":"Mitochondrial Signaling Pathways Associated with DNA Damage Responses","volume":"24","author":"Shimura T","year":"2023","journal-title":"Int J Mol Sci"},{"issue":"5","key":"ref9","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbamcr.2022.119233","article-title":"The Fate of Damaged Mitochondrial DNA in the Cell","volume":"1869","author":"Liao S","year":"2022","journal-title":"Biochim Biophys Acta Mol Cell Res"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-023-41304-8","article-title":"The Master Energy Homeostasis Regulator PGC-1\u03b1 Exhibits an mRNA Nuclear Export Function","volume":"14","author":"Mihaylov SR","year":"2023","journal-title":"Nat Commun"},{"key":"ref11","article-title":"Peroxisom Proliferator-Activated Receptor-\u03b3 Coactivator-1\u03b1 in Neurodegenerative Disorders: A Promising Therapeutic Target","volume":"215","author":"Yang YN","year":"2023","journal-title":"Biochem Pharmacol"},{"key":"ref12","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2020.00349","article-title":"A Review of the Pharmacological Action of Astragalus Polysaccharide","volume":"11","author":"Zheng Y","year":"2020","journal-title":"Front Pharmacol"},{"issue":"6","key":"ref13","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/s12272-022-01393-3","article-title":"Astragalus Polysaccharide: A Review of its Immunomodulatory Effect","volume":"45","author":"Li CX","year":"2022","journal-title":"Arch Pharm Res"},{"key":"ref14","article-title":"Pharmacological Effects of Astragalus Polysaccharides in Treating Neurodegenerative Diseases","volume":"15","author":"Shi Y","year":"2024","journal-title":"Front Pharmacol"},{"key":"ref15","doi-asserted-by":"crossref","DOI":"10.1016\/j.tifs.2024.104707","article-title":"Recent Advances in Astragalus Polysaccharides: Structural Characterization, Bioactivities and Gut Microbiota Modulation Effects","volume":"153","author":"Chen T","year":"2024","journal-title":"Trends Food Sci Technol"},{"key":"ref16","article-title":"Myocardial Ischemia-Reperfusion Injury; Molecular Mechanisms and Prevention","volume":"149","author":"Liu Y","year":"2023","journal-title":"Microvasc Res"},{"key":"ref17","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2025.123119","article-title":"Mitigation of Ischemia\/Reperfusion Injury Via Selenium Nanoparticles: Suppression of STAT1 to Inhibit Cardiomyocyte Oxidative Stress and Inflammation","volume":"318","author":"Chen C","year":"2025","journal-title":"Biomaterials"},{"issue":"1","key":"ref18","article-title":"Ischemia-Reperfusion Injury: Molecular Mechanisms and Therapeutic Targets","volume":"9","author":"Zhang M","year":"2024","journal-title":"Signal Transduct Target Ther"},{"issue":"2","key":"ref19","article-title":"APPL1 Ameliorates Myocardial Ischemia-Reperfusion Injury by Regulating the AMPK Signaling Pathway","volume":"23","author":"Cen Y","year":"2022","journal-title":"Exp Ther Med"},{"issue":"5","key":"ref20","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1089\/jmf.2022.0022","article-title":"Natural Products as Modulators of Mitochondrial Dysfunctions Associated with Cardiovascular Diseases: Advances and Opportunities","volume":"26","author":"Boeing T","year":"2023","journal-title":"J Med Food"},{"key":"ref21","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2025.119588","article-title":"Mitochondria as Therapeutic Targets for Natural Products in the Treatment of Cardiovascular Diseases","volume":"345","author":"Yang Y","year":"2025","journal-title":"J Ethnopharmacol"},{"issue":"1","key":"ref22","doi-asserted-by":"crossref","DOI":"10.3390\/ijms26010380","article-title":"Plant Secondary Metabolites as Modulators of Mitochondrial Health: An Overview of Their Anti-Oxidant, Anti-Apoptotic, and Mitophagic Mechanisms","volume":"26","author":"Anchimowicz J","year":"2025","journal-title":"Int J Mol Sci"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"2.26.1","DOI":"10.1002\/cptx.21","article-title":"Quantification of Lactate Dehydrogenase for Cell Viability Testing Using Cell Lines and Primary Cultured Astrocytes","volume":"72","author":"Kaja S","year":"2017","journal-title":"Curr Protoc Toxicol"},{"issue":"1","key":"ref24","article-title":"GSK3\u00df-Mediated Tau Hyperphosphorylation Triggers Diabetic Retinal Neurodegeneration by Disrupting Synaptic and Mitochondrial Functions","volume":"13","author":"Zhu H","year":"2018","journal-title":"Mol Neurodegener"},{"issue":"5","key":"ref25","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbadis.2024.167158","article-title":"Cholesterol 25-Hydroxylase Prevents Type 2 Diabetes Mellitus Induced Cardiomyopathy by Alleviating Cardiac Lipotoxicity","volume":"1870","author":"Zhang J","year":"2024","journal-title":"Biochim Biophys Acta Mol Basis Dis"},{"issue":"9","key":"ref26","doi-asserted-by":"crossref","first-page":"8350","DOI":"10.3168\/jds.2019-17444","article-title":"Staphylococcal Enterotoxin M Induced Inflammation and Impairment of Bovine Mammary Epithelial Cells","volume":"103","author":"Zhao Y","year":"2020","journal-title":"J Dairy Sci"},{"issue":"1","key":"ref27","article-title":"Mitochondrial Dysfunction: Mechanisms and Advances in Therapy","volume":"9","author":"Zong Y","year":"2024","journal-title":"Signal Transduct Target Ther"},{"issue":"5","key":"ref28","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1016\/j.bbadis.2016.11.010","article-title":"Mitochondrial Dysfunction and Oxidative Stress in Metabolic Disorders - A Step Towards Mitochondria Based Therapeutic Strategies","volume":"1863","author":"Bhatti JS","year":"2017","journal-title":"Biochim Biophys Acta Mol Basis Dis"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"2209","DOI":"10.2147\/DMSO.S228351","article-title":"Protective Effects of Astragalus Polysaccharides on Oxidative Stress in High Glucose-Induced or SOD2-Silenced H9C2 Cells Based on PCR Array Analysis","volume":"12","author":"Sun Q","year":"2019","journal-title":"Diabetes Metab Syndr Obes"},{"issue":"3","key":"ref30","doi-asserted-by":"crossref","first-page":"4837","DOI":"10.18632\/oncotarget.13596","article-title":"Astragalus Polysaccharide Restores Autophagic Flux and Improves Cardiomyocyte Function in Doxorubicin-Induced Cardiotoxicity","volume":"8","author":"Cao Y","year":"2017","journal-title":"Oncotarget"},{"issue":"2","key":"ref31","doi-asserted-by":"crossref","DOI":"10.3390\/cells10020352","article-title":"Dysregulation of PGC-1\u03b1-Dependent Transcriptional Programs in Neurological and Developmental Disorders: Therapeutic Challenges and Opportunities","volume":"10","author":"McMeekin LJ","year":"2021","journal-title":"Cells"},{"issue":"5","key":"ref32","doi-asserted-by":"crossref","DOI":"10.3390\/antiox12051075","article-title":"PGC-1\u03b1 is a Master Regulator of Mitochondrial Lifecycle and ROS Stress Response","volume":"12","author":"Abu Shelbayeh O","year":"2023","journal-title":"Antioxidants"},{"key":"ref33","doi-asserted-by":"crossref","DOI":"10.1155\/2020\/1452696","article-title":"PGC-1\u03b1, Inflammation, and Oxidative Stress: An Integrative View in Metabolism","volume":"2020","author":"Rius-P\u00e9rez S","year":"2020","journal-title":"Oxid Med Cell Longev"},{"issue":"4","key":"ref34","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1038\/s41580-022-00547-x","article-title":"New Insights into Activation and Function of the AMPK","volume":"24","author":"Steinberg GR","year":"2023","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"9","key":"ref35","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1038\/ncb2329","article-title":"The AMPK Signalling Pathway Coordinates Cell Growth, Autophagy and Metabolism","volume":"13","author":"Mihaylova MM","year":"2011","journal-title":"Nat Cell Biol"}],"container-title":["Arquivos Brasileiros de Cardiologia"],"original-title":[],"link":[{"URL":"http:\/\/www.scielo.br\/scielo.php?script=sci_pdf&pid=S0066-782X2026000800607&tlng=pt","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/abccardiol.org\/wp-content\/uploads\/articles_xml\/0066-782x-abc-123-8-e20250854\/0066-782x-abc-123-8-e20250854.x69353.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:29:43Z","timestamp":1791916183000},"score":1,"resource":{"primary":{"URL":"https:\/\/abccardiol.org\/article\/o-polissacarideo-de-astragalus-melhora-a-biogenese-e-a-funcao-mitocondrial-em-cardiomiocitos-atraves-da-ativacao-da-ampk\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":35,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026]]}},"alternative-id":["S0066-782X2026000800607"],"URL":"https:\/\/doi.org\/10.36660\/abc.20250854","relation":{"has-translation":[{"id-type":"doi","id":"10.36660\/abc.20250854i","asserted-by":"subject"},{"id-type":"doi","id":"10.36660\/abc.20250854i","asserted-by":"object"}]},"ISSN":["1678-4170","0066-782X"],"issn-type":[{"value":"1678-4170","type":"electronic"},{"value":"0066-782X","type":"print"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"e20250854"}}