{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:48:17Z","timestamp":1791917297990,"version":"4.3.4"},"reference-count":30,"publisher":"Sociedade Brasileira de Cardiologia","issue":"8","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arq. Bras. Cardiol."],"abstract":"<jats:p>Resumo  Fundamento A disfun\u00e7\u00e3o endotelial e a ades\u00e3o de mon\u00f3citos induzidas por \u00e1cidos graxos livres (AGLs) s\u00e3o eventos iniciais cr\u00edticos na aterosclerose. O palmitato, um \u00e1cido graxo livre saturado de cadeia longa, aumentou a express\u00e3o de GPR84. Embora o GPR84 seja canonicamente ativado por AGLs de cadeia m\u00e9dia, sua indu\u00e7\u00e3o sob estresse lipot\u00f3xico sugere um papel compensat\u00f3rio e dependente do contexto. Seu papel na inflama\u00e7\u00e3o endotelial ainda n\u00e3o est\u00e1 bem definido.  Objetivos Investigar o papel funcional da ativa\u00e7\u00e3o do GPR84 na inflama\u00e7\u00e3o endotelial induzida por AGLs e elucidar os mecanismos subjacentes.  M\u00e9todos C\u00e9lulas endoteliais da aorta humana (HAECs) foram desafiadas com palmitato (0,5 ou 1 mM) na presen\u00e7a ou aus\u00eancia dos agonistas de GPR84, 6-OAU ou ZQ-16 (cada um a 1 \u03bcM). A express\u00e3o de citocinas inflamat\u00f3rias e mol\u00e9culas de ades\u00e3o foi avaliada por qPCR e ELISA. A ades\u00e3o de mon\u00f3citos foi avaliada utilizando c\u00e9lulas THP-1. A transloca\u00e7\u00e3o nuclear de NF-\u03baB p65 e a express\u00e3o de fator de crescimento independente 1 (Gfi1) foram examinadas, e o silenciamento de Gfi1 foi realizado para avaliar a relev\u00e2ncia funcional.  Resultados O palmitato aumentou a express\u00e3o de GPR84 em HAECs. O tratamento com agonistas de GPR84 reduziu significativamente a superexpress\u00e3o induzida por palmitato de IL-1\u03b2, TNF-\u03b1, MCP-1, VCAM-1 e ICAM-1, tanto em n\u00edvel de mRNA quanto de prote\u00edna, e atenuou a ades\u00e3o de mon\u00f3citos THP-1. Mecanisticamente, a ativa\u00e7\u00e3o de GPR84 inibiu a transloca\u00e7\u00e3o nuclear de NF-\u03baB p65 e preservou a express\u00e3o de Gfi1. O silenciamento de Gfi1 anulou os efeitos protetores dos agonistas de GPR84, restaurando a ativa\u00e7\u00e3o de NF-\u03baB, a express\u00e3o de mol\u00e9culas de ades\u00e3o e a ades\u00e3o de mon\u00f3citos.  Conclus\u00f5es O agonismo do GPR84 atenua a inflama\u00e7\u00e3o endotelial induzida por AGLs ao restaurar a express\u00e3o de Gfi1 e suprimir a sinaliza\u00e7\u00e3o de NF-\u03baB, destacando uma potencial estrat\u00e9gia terap\u00eautica para aterosclerose precoce que justifica valida\u00e7\u00e3o in vivo adicional.<\/jats:p>","DOI":"10.36660\/abc.20260231","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:19:27Z","timestamp":1791915567000},"source":"Crossref","is-referenced-by-count":0,"title":["O Agonismo do GPR84 Atenua a Inflama\u00e7\u00e3o Endotelial Induzida por \u00c1cidos Graxos Livres e a Ades\u00e3o de Mon\u00f3citos atrav\u00e9s da Via Gfi1\/NF-\u039ab"],"prefix":"10.36660","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-5796-7485","authenticated-orcid":false,"given":"Li","family":"Li","sequence":"first","affiliation":[{"name":"The Third People\u2019s Hospital of Longgang District Shenzhen","place":["Guangdong Province, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5731-6261","authenticated-orcid":false,"given":"Gang","family":"Fu","sequence":"additional","affiliation":[{"name":"The Third People\u2019s Hospital of Longgang District Shenzhen","place":["Guangdong Province, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3243-2307","authenticated-orcid":false,"given":"Yanling","family":"Liu","sequence":"additional","affiliation":[{"name":"The Third People\u2019s Hospital of Longgang District Shenzhen","place":["Guangdong Province, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9288-7889","authenticated-orcid":false,"given":"Caiyun","family":"Liu","sequence":"additional","affiliation":[{"name":"The Third People\u2019s Hospital of Longgang District Shenzhen","place":["Guangdong Province, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"22636","published-online":{"date-parts":[[2026]]},"reference":[{"issue":"25","key":"ref1","first-page":"2982","article-title":"Global Burden of Cardiovascular Diseases and Risk Factors, 1990-2019: Update from the GBD 2019 Study","volume":"76","author":"Roth GA","year":"2020","journal-title":"J Am Coll Cardiol"},{"issue":"10","key":"ref2","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1038\/nri2171","article-title":"Evolving Functions of Endothelial Cells in Inflammation","volume":"7","author":"Pober JS","year":"2007","journal-title":"Nat Rev Immunol"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/s11154-010-9134-4","article-title":"Endothelial Dysfunction in Diabetes Mellitus: Molecular Mechanisms and Clinical Implications","volume":"11","author":"Tabit CE","year":"2010","journal-title":"Rev Endocr Metab 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