{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T05:31:56Z","timestamp":1734499916404,"version":"3.30.2"},"reference-count":27,"publisher":"ALUNA","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:p>Aim: To investigate changes in oxidative stress indicators in rats under conditions of long-term ethanol exposure.\nMaterials and Methods: We studied the effect of prolonged exposure to ethanol on the activity of free radical processes in the gonads of rats of both sexes. Experimental animals were divided into 2 groups: Group I (control group) rats, which were injected with distilled water orally for 28 days; II group \u2013 which for 28 days were injected intragastrically with a 30% ethanol solution at the rate of 2ml\/100g of body weight once a day.\nResults: The conducted experimental studies showed that the level of free radicals increases in animals that were injected with ethanol for 28 days, as indicated by an increase in the ROS index by 1.6 times in males and 1.7 times in females. Lipid peroxidation processes are also activated, as evidenced by an increase in the level of initial products of the lipoperoxidation chain \u2013 HPL by 54.6%, intermediate products \u2013 TBP by 57.9% and final products \u2013 SHO \u2013 by 80.3% in males and by 68.2%, respectively , 80.9% and 84.6% in females. Along with this, the activity of oxidative modification of proteins in the organs of the reproductive system of the experimental group of animals also increased. In particular, the level of OM\u0420370 in the testes of male rats increased by 89.1%, and OM\u0420430 increased by 56.4% from the level of animals that were not given ethanol. In the ovaries of female rats, the level of OM\u0420370 increased by 112.%, and OM\u0420430 increased by 60.7% from the level of animals without simulating ethanol intoxication. Therefore, we were able to establish significant changes and disturbances in the processes of free radical oxidation in the organs of the reproductive system of animals under the toxic effect of ethanol, which can negatively affect the reproductive function and quality of offspring in animals that were simulated ethanol intoxication.\nConclusions: In animals of both sexes, under the conditions of chronic ethanol exposure, there is an increase in free radical oxidation indicators, namely ROS, lipid peroxidation, oxidative modification of proteins, and the growth indicators in females are more significant than in males. This indicates a negative effect of chronic ethanol intoxication on the state of the membrane structures of germ cells, with DNA damage, which can lead to a violation of the function of the genital organs.<\/jats:p>","DOI":"10.36740\/merkur202405104","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T13:15:24Z","timestamp":1734441324000},"page":"500-504","source":"Crossref","is-referenced-by-count":0,"title":["Indicators of oxidative stress in the organs of the reproductive system of rats under conditions of long-term ethanol exposure"],"prefix":"10.36740","volume":"52","author":[{"given":"Sergiy O.","family":"Nesteruk","sequence":"first","affiliation":[{"name":"I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY, TERNOPIL, UKRAINE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaroslav O.","family":"Bilyk","sequence":"additional","affiliation":[{"name":"I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY, TERNOPIL, UKRAINE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan M.","family":"Klishch","sequence":"additional","affiliation":[{"name":"I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY, TERNOPIL, UKRAINE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oksana M.","family":"Matolinets","sequence":"additional","affiliation":[{"name":"DANYLO GALYTSKY LVIV NATIONAL MEDICAL UNIVERSITY, LVIV, UKRAINE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olena A.","family":"Yakymchuk","sequence":"additional","affiliation":[{"name":"I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY, TERNOPIL, UKRAINE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viktor V.","family":"Tverdochlib","sequence":"additional","affiliation":[{"name":"I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY, TERNOPIL, UKRAINE"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"22436","published-online":{"date-parts":[[2024]]},"reference":[{"key":"ref0","unstructured":"1. Kharchenko O, Gavrysh H, Ostapchenko L. Toxic effects of ethanol and its products on the organism. Visnyk of the National Academy of Sciences of Ukraine. 2006;3:57-64."},{"key":"ref1","doi-asserted-by":"crossref","unstructured":"2. Zakhari S. Overview: how is alcohol metabolized by the body? Alcohol. Res. Health. 2006:29:245-254.","DOI":"10.1159\/000095013"},{"key":"ref2","unstructured":"3. Shayakhmetova GM. Ethanol-induced metabolic rearrangement: Consequences for reproductive function. Pharmacology and Drug Toxicology. 2017;6:3-14."},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"4. Lieber CS. Metabolism of alcohol. Clin. Liver Dis. 2005;9(1):1-35.","DOI":"10.1016\/j.cld.2004.10.005"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"5. Orpana AK, Orava MM, VihkoRK, et al. Ethanol-induced inhibition of testosterone biosynthesis in rat Leydig cells: central role of mitochondrial NADH redox state. J. Steroid. Biochem. 1990;36(6):603-608.","DOI":"10.1016\/0022-4731(90)90179-v"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"6. Quintans LN, Castro GD, Castro JA. Oxidation of ethanol to acetaldehyde and free radicals by rat testicular microsomes. Arch. Toxicol. 2005;79:25-30.","DOI":"10.1007\/s00204-004-0609-5"},{"key":"ref6","unstructured":"7. Wu D, Cederbaum AI. Alcohol, oxidative stress, and free radical damage. Alcohol. Res. Health. 2003;27:277-284."},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"8. Grattagliano I, Vendemiale G, Errico et al. Chronic ethanol intake induces oxidative alterations in rat testis. J. Appl. Toxicol. 1997;17(5):307-311.","DOI":"10.1002\/(SICI)1099-1263(199709)17:5<307::AID-JAT446>3.0.CO;2-Q"},{"key":"ref8","doi-asserted-by":"publisher","unstructured":"9. Galardo MN, Regueira M, Riera MF et al. Lactate Regulates Rat Male Germ Cell Function through Reactive Oxygen Species. PLOS one. 2014;9(1):e88024.","DOI":"10.1371\/journal.pone.0088024"},{"key":"ref9","doi-asserted-by":"publisher","unstructured":"10. Jana K, Jana N, De DK, Guha SK. Ethanol induces mouse spermatogenic cell apoptosis in vivo through over-expression of Fas\/Fas-L, p53, and caspase-3 along with cytochrome c translocation and glutathione depletion. Mol. Reprod. Dev. 2010;77(9):820-833.","DOI":"10.1002\/mrd.21227"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"11. Guerriero G, Trocchia S, Abdel-Gawad FK, Ciarcia G. Roles of reactive oxygen species in the spermatogenesis regulation. Frontiers in endocrinology. 2014;5:91546.","DOI":"10.3389\/fendo.2014.00056"},{"key":"ref11","doi-asserted-by":"publisher","unstructured":"12. Aitken RJ, Smith TB, Jobling MS, Baker MA, De Iuliis GN. Oxidative stress and male reproductive health. Asian J Androl. 2014;16(1):31-8. doi:10.4103\/1008-682X.122203.","DOI":"10.4103\/1008-682x.122203"},{"key":"ref12","doi-asserted-by":"publisher","unstructured":"13. Guerriero G, Trocchia S, Abdel-Gawad FK, Ciarcia G. Roles of reactive oxygen species in the spermatogenesis regulation. Front. Endocrinol. (Lausanne).2014;5:1-4.","DOI":"10.3389\/fendo.2014.00056"},{"key":"ref13","unstructured":"14. European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Council of Europe. Strasbourg. 1986;123:52 p. https:\/\/rm.coe.int\/168007a67b"},{"key":"ref14","unstructured":"15. Nesteruk SO, Klishch IM. Pokaznyky endohennoyi intoksykatsiyi shchuriv za umov khronichnoyi alkohol'noyi intoksykatsiyi [Indicators of endogenous intoxication in rats under conditions of chronic alcohol intoxication]. Medical and Clinical Chemistry. 2023;1:29-33."},{"key":"ref15","unstructured":"16. Vlizlo VV, et al. Laboratory research methods in biology, animal husbandry, and veterinary medicine: A reference book. Lviv: Spolom. 2012, p.764."},{"key":"ref16","doi-asserted-by":"publisher","unstructured":"17. Nesteruk SO, Hnatiuk MS, Fedoniuk LYa, Smachylo II, Krynytska IYa, Marushchak MI. The structural rearrangement of testicular arteries in case of chroni\u0107 ethanol intoxication. Paediatric Surgery (Ukraine). 2023;4(81):81-86. doi: 10.15574\/PS.2023.81.81.","DOI":"10.15574\/ps.2023.81.81"},{"key":"ref17","unstructured":"18. Meshchyshen IF. Method for determining oxidative modification of plasma (serum) proteins. Bukovynian Medical Bulletin. 1998;2(1):156-158."},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"19. Nabil E, Yuko I, Yoshinori O. Involvement of inducible nitric oxide synthase in DNA fragmentation in various testicular germ cells of ethanol-treated rats. Journal of Men\u2019s Health. 2011;8(1):S36-S40.","DOI":"10.1016\/s1875-6867(11)60018-3"},{"key":"ref19","unstructured":"20. Baraboy VA, Reznikov AG. Physiology, biochemistry and psychology of stress. Kyiv: Interservice. 2013, p.314. doi: 10.5281\/zenodo.3470144. (Ukrainian)"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"21. Reznikov AG. Oxidative stress as a mandatory participant in the pathogenesis of stress-induced reproductive disorders. Endokrynologia. 2023;28(4):341-8. doi: 10.31793\/1680-1466.2023.28-4.341. (Ukrainian)","DOI":"10.31793\/1680-1466.2023.28-4.341"},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"22. Fanning JR, Lee R, Gozal D, Coussons-Read M, Coccaro EF. Childhood trauma and parental style: Relationship with markers of inflammation, oxidative stress, and aggression in healthy and personality disordered subjects. Biol Psychol. 2015;112:56-65. doi: 10.1016\/j.biopsycho.2015.09.003.","DOI":"10.1016\/j.biopsycho.2015.09.003"},{"key":"ref22","doi-asserted-by":"publisher","unstructured":"23. Alameda L, Fournier M, Khadimallah I, Griffa A, Cleusix M, Jenni R, et al. Redox dysregulation as a link between childhood trauma and psychopathological and neurocognitive profile in patients with early psychosis. Proc Natl Acad Sci USA. 2018;115(49):12495-500. doi: 10.1073\/pnas.1812821115.","DOI":"10.1073\/pnas.1812821115"},{"key":"ref23","doi-asserted-by":"publisher","unstructured":"24. Karanikas E, Daskalakis NP, Agorastos A. Oxidative dysregulation in early life stress and posttraumatic stress disorder: A comprehensive review. Brain Sci. 2021;11(6):723. doi: 10.3390\/brainsci11060723.","DOI":"10.3390\/brainsci11060723"},{"key":"ref24","doi-asserted-by":"publisher","unstructured":"25. Almeida SA, Kempinas WG, Lamano Carvalho TL. Sexual behav-ior and fertility of male rats submitted to prolonged immobilization-induced stress. Braz J Med Biol Res. 2000;33(9):1105-9. doi: 10.1590\/s0100-879x2000000900019.","DOI":"10.1590\/s0100-879x2000000900019"},{"key":"ref25","doi-asserted-by":"publisher","unstructured":"26. Almeida SA, Petenusci SO, Anselmo-Franci JA, Rosa e Silva AA, Carvalho TL. Chronic immobilization-induced stress increases plasma testosterone and delays testicular maturation in pubertal rats. Andrologia. 2000;32(1):7-11.","DOI":"10.1111\/j.1439-0272.2000.tb02858.x"},{"key":"ref26","doi-asserted-by":"publisher","unstructured":"27. Almeida SA, Petenusci SO, Anselmo-Franci JA, Rosa-e-Silva AA, Lamano-Carvalho TL. Decreased spermatogenic and andro-genic testicular functions in adult rats submitted to immobilization-induced stress from prepuberty. Braz J Med Biol Res. 1998;31(11):1443-8. doi: 10.1590\/s0100-879x1998001100013.","DOI":"10.1590\/s0100-879x1998001100013"}],"container-title":["Polski Merkuriusz Lekarski"],"original-title":[],"language":"en","deposited":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T13:15:33Z","timestamp":1734441333000},"score":1,"resource":{"primary":{"URL":"https:\/\/polskimerkuriuszlekarski.pl\/wp-content\/uploads\/library\/PolMerkurLek2024i5net.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":27,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2024]]},"published-print":{"date-parts":[[2024,10]]}},"URL":"https:\/\/doi.org\/10.36740\/merkur202405104","archive":["Internet Archive"],"relation":{},"ISSN":["1426-9686"],"issn-type":[{"value":"1426-9686","type":"print"}],"subject":[],"published":{"date-parts":[[2024]]}}}