{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T14:59:20Z","timestamp":1778511560211,"version":"3.51.4"},"reference-count":32,"publisher":"ALUNA","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Wiadomo\u015bci Lekarskie"],"abstract":"Aim<\/jats:title>To improve the results of treatment of patients with chronic wounds of the lower extremities by using complex treatment, including surgical interventions, VAC- therapy, as well as studying the effect of negative pressure on bacterial films of wounds, based on microbiological examination and immune-histochemical data.<\/jats:p><\/jats:sec>Material and methods<\/jats:title>During the period from 2019 to 2023 at the department's clinic, 68 patients with chronic wounds of the lower extremities were examined and treated. These are mainly women (n=63) aged from 35 to 80 years.<\/jats:p><\/jats:sec>Results<\/jats:title>Complete wound healing was achieved in all patients. In 79% of patients, the transplanted skin graft took root completely. Partial lysis was observed in 17%, requiring repeated operations. In 4%, the wounds healed without surgery. The VAC- therapy we used prevented the restoration of the biofilm and prevented further infection of the wound. After complex treatment, we observed positive dynamics of clinical and immunological parameters. Indicators of T-cell immunity (CD3+, CD4+, CD19+) were restored, the level of CIC significantly decreased and the level of IgM increased. The level of interleukins on day 21 showed a tendency towards normalization.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>A modern integrated approach gives the chance to radically solve the problems of healing chronic wounds. VAC- therapy in combination with surgical treatment and antibacterial therapy prevented the restoration of biofilm and further infection of wounds, which led to the wound healing in 100% cases.<\/jats:p><\/jats:sec>","DOI":"10.36740\/wlek\/195551","type":"journal-article","created":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T08:40:46Z","timestamp":1738312846000},"page":"2457-2463","source":"Crossref","is-referenced-by-count":1,"title":["Treatment of chronic wounds of the lower limb with the use of VAC-therapy. Impact on immunity and bacterial films"],"prefix":"10.36740","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7929-7350","authenticated-orcid":false,"given":"Igor Vasiliovich","family":"Korpusenco","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6468-6297","authenticated-orcid":false,"given":"Nadiia Nikolaevna","family":"Nor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8318-6239","authenticated-orcid":false,"given":"Valery Pavlovich","family":"Kryshen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0643-5884","authenticated-orcid":false,"given":"Valentina Euvgenivna","family":"Kudryavtseva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4018-951X","authenticated-orcid":false,"given":"Olena Igorivna","family":"Korpusenco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6892-2314","authenticated-orcid":false,"given":"Olexander Mikolayovich","family":"Makarenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9253-6097","authenticated-orcid":false,"given":"Vasyl Vasyliovich","family":"Koshtura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"22436","published-online":{"date-parts":[[2024,12,30]]},"reference":[{"key":"ref-1358244","unstructured":"D\u2019Atanasio N et al. 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