{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T05:19:05Z","timestamp":1738387145012,"version":"3.35.0"},"reference-count":33,"publisher":"ALUNA","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Wiadomo\u015bci Lekarskie"],"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>Background: urinary bladder cancer is a disturbing malignant disorder which participates about 3% to the recent development of cancer field. The etiology and pathogenesis can be related to environmental and occupational agents. Transformation of normal urothelial cell to a neoplastic one occurs through complicated sequences of molecular proceedings termed as epithelial plasticity. Aims: to analyze expression levels of GATA-3 in bladder tumor tissues and to prove a relation between expression of GATA-3 and clinicopathological characteristics of bladder tumors, including patient age, sex, tumor grade, and muscle invasion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Materials and methods: Forty Formalin fixed paraffin embedded FFPE tissue blocks obtained from bladder tumor by transurethral resection are collected from teaching hospitals at Al-Najaf governorate. Those blocks are stained by using hematoxylin and eosin stain. Histopathological features were examined and then immunostained by GATA-3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Results: Evaluation of GATA-3 expression in urothelial carcinoma, revealed GATA-3 test was (positive) in thirty-four samples of urothelial carcinoma, while GATA-3 test was (negative) in twenty-one samples of urothelial carcinoma. Correlation of GATA-3 with other variables (age, sex, and grade) was a statistically non-significant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Conclusion: GATA-3 is an Immunohistochemical sensitive marker to diagnose urothelial carcinoma. GATA-3 expression showed non-significant relation with age, gender and histopathological parameters and its expression has been observed to be lost or reduced in substantial proportion in relation to urothelial carcinoma. This alteration or down regulation of GATA-3 is correlated with higher tumor grade and stage.<\/jats:p><\/jats:sec>","DOI":"10.36740\/wlek\/195858","type":"journal-article","created":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T08:40:46Z","timestamp":1738312846000},"page":"2381-2387","source":"Crossref","is-referenced-by-count":0,"title":["Immunohistochemical Evaluation of GATA-3 in Patients with Urinary Bladder Cancer"],"prefix":"10.36740","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-5281-8857","authenticated-orcid":false,"given":"Randa Saad","family":"Hameed","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4997-5549","authenticated-orcid":false,"given":"Rihab Hameed","family":"Almudhafar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2310-2419","authenticated-orcid":false,"given":"Liwaa Hussein","family":"Mahdi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"22436","published-online":{"date-parts":[[2025,1,30]]},"reference":[{"key":"ref-1358000","doi-asserted-by":"publisher","DOI":"10.1007\/s00345-019-02984-4"},{"key":"ref-1358001","doi-asserted-by":"publisher","DOI":"10.1016\/j.mpsur.2019.07.003"},{"key":"ref-1358002","doi-asserted-by":"publisher","DOI":"10.25259\/IJMS_159_2020"},{"key":"ref-1358003","doi-asserted-by":"publisher","DOI":"10.4103\/IJPM.IJPM_228_18"},{"key":"ref-1358004","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"ref-1358005","doi-asserted-by":"crossref","unstructured":"Guo CC, Bondaruk J, Yao H, Wang Z, Zhang L, Lee S, et al. Assessment of luminal and basal phenotypes in bladder cancer.Sci Rep. 2020; 10: 9743. Article number: 9743 (2020).","DOI":"10.1038\/s41598-020-66747-7"},{"key":"ref-1358006","doi-asserted-by":"publisher","DOI":"10.1007\/s11684-019-0687-7"},{"key":"ref-1358007","doi-asserted-by":"publisher","DOI":"10.1016\/j.anndiagpath.2019.08.001"},{"key":"ref-1358008","doi-asserted-by":"crossref","unstructured":"Wang CC, Tsai YC, Jeng YM. Biological significance of GATA3, cytokeratin 20, cytokeratin 5\/6 and p53 expression in muscle-invasive bladder cancer. PLoS One. 2019; 14: Article e0221785.","DOI":"10.1371\/journal.pone.0221785"},{"key":"ref-1358009","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-019-02618-5"},{"key":"ref-1358010","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-019-6042-1"},{"key":"ref-1358011","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S237099"},{"key":"ref-1358012","doi-asserted-by":"crossref","unstructured":"Goto T, Miyamoto H. The role of estrogen receptors in urothelial cancer. Front Endocrinol (Lausanne). 2021; 12(2021), Article 643870.","DOI":"10.3389\/fendo.2021.643870"},{"key":"ref-1358013","doi-asserted-by":"publisher","DOI":"10.3390\/medsci8010015"},{"key":"ref-1358014","doi-asserted-by":"crossref","unstructured":"Agarwal H, Babu S, Rana C, et al. Diagnostic utility of GATA3 immunohistochemical expression in urothelial carcinoma. Indian J Pathol Microbiol. 2019; 62(2): 244-50.","DOI":"10.4103\/IJPM.IJPM_228_18"},{"key":"ref-1358015","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.00623"},{"key":"ref-1358016","doi-asserted-by":"crossref","unstructured":"Jangir H, Nambirajan A, Ranjit AS, et al. Prognostic stratification of muscle invasive urothelial carcinomas using limited immunohistochemical panel of Gata3 and cytokeratin\u2019s 5\/6, 14 and 20. Ann Diagn Pathol. 2019; (43): 151397.","DOI":"10.1016\/j.anndiagpath.2019.08.001"},{"key":"ref-1358017","doi-asserted-by":"crossref","unstructured":"Sung H, Ferlay J, Siegel RL, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA. (2021) 71:209\u201349. 10.3322\/caac.21660.","DOI":"10.3322\/caac.21660"},{"key":"ref-1358018","doi-asserted-by":"crossref","unstructured":"Witjes JA, Bruins HM, Cathomas R et al. European association of urology guidelines on muscle-invasive and metastatic bladder cancer: summary of the 2020 guidelines. Eur Urol. 2021; 79:82\u2013104.","DOI":"10.1016\/j.eururo.2020.03.055"},{"key":"ref-1358019","doi-asserted-by":"crossref","unstructured":"Kamoun A, de Reynies A, Allory Y, et al. A consensus molecular classification of muscle-invasive bladder cancer. Eur Urol. 2019; 77: 420\u201333.","DOI":"10.1016\/j.eururo.2019.09.006"},{"key":"ref-1358020","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-00628-5"},{"key":"ref-1358021","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2021.684140"},{"key":"ref-1358022","doi-asserted-by":"crossref","unstructured":"Lotan Y, de Jong JJ, Liu VYT, et al. Patients with muscle-invasive bladder cancer with nonluminal subtype derives greatest benefit from platinum based neoadjuvant chemotherapy. J Urol. 2021; 207: 541\u201350. 10.1097\/JU.0000000000002261.","DOI":"10.1097\/JU.0000000000002261"},{"key":"ref-1358023","doi-asserted-by":"crossref","unstructured":"Guo WCC, Bondaruk J, Yao H, et al. Assessment of luminal and basal phenotypes in bladder cancer. Sci Rep. 2020; 10:9743. 10.1038\/s41598-020-66747-7.","DOI":"10.1038\/s41598-020-66747-7"},{"key":"ref-1358024","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-66747-7"},{"key":"ref-1358025","doi-asserted-by":"crossref","unstructured":"Bejrananda T, Kanjanapradit K, Saetang J, Sangkhathat S. Impact of immunohistochemistry-based subtyping of GATA3, CK20, CK5\/6, and CK14 expression on survival after radical cystectomy for muscle-invasive bladder cancer. Sci Rep. 2021; 11: 21186. 10.1038\/s41598-021-00628-5.","DOI":"10.1038\/s41598-021-00628-5"},{"key":"ref-1358026","doi-asserted-by":"crossref","unstructured":"Kim J, Kwiatkowski D, McConkey DJ, Meeks JJ, Freeman SS, Bellmunt J, et al. The cancer genome atlas expression subtypes stratify response to checkpoint inhibition in advanced urothelial cancer and identify a subset of patients with high survival probability. Eur Urol. 2019; 75: 961\u20134. 10.1016\/j.eururo.2019.02.017.","DOI":"10.1016\/j.eururo.2019.02.017"},{"key":"ref-1358027","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2021.10.035"},{"key":"ref-1358028","doi-asserted-by":"crossref","unstructured":"Taber A, Christensen E, Lamy P, Nordentoft I, Prip F, Lindskrog SV, et al. Molecular correlates of cisplatin-based chemotherapy response in muscle invasive bladder cancer by integrated multi-omics analysis. Nat Commun. 2020; 11: 4858. Article number: 4858 (2020).","DOI":"10.1038\/s41467-020-18640-0"},{"key":"ref-1358029","doi-asserted-by":"crossref","unstructured":"Kollberg P, Chebil G, Eriksson P, Sj\u00f6dahl G, Liedberg F. Molecular subtypes applied to a population-based modern cystectomy series do not predict cancer-specific survival. Urol Oncol. 2019; 37: 791\u20139. 10.1016\/j.urolonc.2019.04.010.","DOI":"10.1016\/j.urolonc.2019.04.010"},{"key":"ref-1358030","doi-asserted-by":"publisher","DOI":"10.3390\/medicina59081452"},{"key":"ref-1358031","doi-asserted-by":"crossref","unstructured":"(32) Font A, Domenech M, Benitez R, Rava M, Marques M, Ramirez JL, et al. Immunohistochemistry-based taxonomical classification of bladder cancer predicts response to neoadjuvant chemotherapy. Cancers (Basel). (2020) 12:1784. 10.3390\/cancers12071784.","DOI":"10.3390\/cancers12071784"},{"key":"ref-1358032","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2014.04.015"}],"container-title":["Wiadomo\u015bci Lekarskie"],"original-title":[],"link":[{"URL":"https:\/\/www.wiadomoscilekarskie.pl\/pdf-195858-120977","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.wiadomoscilekarskie.pl\/pdf-195858-120977","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T08:41:03Z","timestamp":1738312863000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.wiadomoscilekarskie.pl\/Immunohistochemical-Evaluation-of-GATA-3-in-Patients-with-Urinary-Bladder-Cancer,195858,0,2.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,30]]},"references-count":33,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2024]]}},"alternative-id":["195858"],"URL":"https:\/\/doi.org\/10.36740\/wlek\/195858","relation":{},"ISSN":["0043-5147","2719-342X"],"issn-type":[{"value":"0043-5147","type":"print"},{"value":"2719-342X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,30]]}}}