{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T01:37:53Z","timestamp":1785116273144,"version":"3.55.0"},"reference-count":0,"publisher":"ALUNA","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Wiadomo\u015bci Lekarskie"],"abstract":"<jats:p>Aim: This study aimed to evaluate the effects of sorafenib on macroglial and microglial activation in the retina under diabetic conditions, using a streptozoto\ncin-\ninduced model of diabetic retinopathy. Special emphasis was placed on examining early and chronic phases of gliosis, assessing molecular markers of glial\nactivation, and determining whether sorafenib can attenuate glial remodelling and neuroinflammation in the diabetic retina.\nMaterials and Methods: Sixty male Wistar rats were divided into three groups: untreated diabetic controls, insulin-treated, and insulin + sorafenib-treated.\nDiabetic retinopathy was induced via intraperitoneal injection of streptozotocin (50 mg\/kg). Retinal samples were collected at 7, 14, 28 days, and 3 months\npost-induction. Histological analysis (H&amp;E staining), immunohistochemistry (GFAP, S100), and Western blotting (GFAP, Iba-1) were used to assess glial activation.\nStatistical analysis was conducted using ANOVA with significance set at p &lt; 0.05.\nResults: Untreated diabetic rats exhibited severe retinal oedema, neurodegeneration, and increased GFAP, S100, and Iba-1 expression, indicating pronounced\nmacroglial and microglial activation. Sorafenib co-treatment significantly reduced the expression of glial markers and preserved retinal structure, with\nnear-complete suppression of gliosis and no evidence of glial-mesenchymal transition. These effects were more pronounced than those of insulin monotherapy.\nConclusions: Sorafenib attenuates retinal glial activation and neuroinflammatory changes in experimental diabetic retinopathy, suggesting its potential as a\nneuroprotective and antifibrotic agent. Targeted kinase inhibition may represent a promising adjunct strategy in early-stage disease management<\/jats:p>","DOI":"10.36740\/wlek\/208991","type":"journal-article","created":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T09:59:06Z","timestamp":1756115946000},"page":"1309-1318","source":"Crossref","is-referenced-by-count":4,"title":["Retinal glial activation in diabetic retinopathy: therapeutic\nimpact of multikinase inhibition with sorafenib"],"prefix":"10.36740","author":[{"given":"Kateryna O.","family":"Usenko","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serhiy V.","family":"Ziablitsev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danylo I.","family":"Yevstifeiev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olena O.","family":"Dyadyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kateryna I.","family":"Popova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"22436","published-online":{"date-parts":[[2025,7,30]]},"container-title":["Wiadomo\u015bci Lekarskie"],"original-title":[],"link":[{"URL":"https:\/\/www.wiadomoscilekarskie.pl\/pdf-208991-128059","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.wiadomoscilekarskie.pl\/pdf-208991-128059","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T09:59:11Z","timestamp":1756115951000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.wiadomoscilekarskie.pl\/Retinal-glial-activation-in-diabetic-retinopathy-therapeutic-nimpact-of-multikinase,208991,0,2.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,30]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025]]}},"alternative-id":["208991"],"URL":"https:\/\/doi.org\/10.36740\/wlek\/208991","relation":{},"ISSN":["0043-5147","2719-342X"],"issn-type":[{"value":"0043-5147","type":"print"},{"value":"2719-342X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,30]]}}}