{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T10:06:52Z","timestamp":1789553212714,"version":"build-2803163510"},"reference-count":0,"publisher":"ALUNA","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Wiadomo\u015bci Lekarskie"],"abstract":"<jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To perform a comprehensive molecular profiling of key apoptotic, inflammatory, and angiogenic markers\u2014p53, IL-6, IL-8, Bax\/Bcl-2 ratio, and VEGF\u2014in peripheral blood samples of patients with prostate cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and methods<\/jats:title>\n                    <jats:p>A total of 60 male participants were enrolled in the study, comprising 40 patients with histologically confirmed prostate cancer and 20 age-matched healthy controls. Patients were stratified according to Gleason score, PSA levels, and tumor stage from the begging of August 2024 to the end May 2025. Blood samples from patients suffering Prostate cancer (PCa). ELISA  Human-specific ELISA kits (e.g., from R&amp;D Systems, BioLegend, or Abcam) (from Serum)  for IL-6, IL-8, VEGF. As well as Bax\/Bcl-2 Ratio.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>P53 Expression, qPCR analysis revealed significantly decreased expression of  p53 mRNA in prostate cancer patients compared to controls (mean \u0394Ct = 6.1 \u00b1 0.8 vs. 4.3 \u00b1 0.6; p &lt; 0.001. Serum IL-6 levels (by ELISA) were elevated in prostate cancer patients (mean = 18.4 \u00b1 6.2 pg\/mL) compared to controls (7.1 \u00b1 2.3 pg\/mL); p &lt; 0.001. Similarly, IL-8 levels were significantly higher in patients (27.5 \u00b1 9.8 pg\/mL) than in controls (10.2 \u00b1 3.5 pg\/mL); p &lt; 0.001. Serum VEGF concentrations were significantly increased in prostate cancer patients (median = 296 pg\/mL; IQR = 230\u2013360 pg\/mL) compared to controls (median = 122 pg\/mL; IQR = 90\u2013160 pg\/mL); p &lt; 0.0001.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Prostate cancer patients exhibited decreased p53 and Bax\/Bcl-2 ratio, indicating impaired apoptosis. IL-6 and IL-8 were significantly elevated and positively correlated, reflecting a pro-inflammatory environment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.36740\/wlek\/220739","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T09:39:33Z","timestamp":1789551573000},"page":"1598-1605","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Profiling of Apoptotic, Inflammatory, and Angiogenic Markers in Prostate Cancer: Focus on p53, IL-6, IL-8, Bax\/Bcl-2 Ratio, and VEGF"],"prefix":"10.36740","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-7484-8296","authenticated-orcid":false,"given":"Mayada Hameed","family":"Al-khafaji","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4690-0057","authenticated-orcid":false,"given":"Wrood Majid","family":"Hameed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhifaf Mohsin","family":"Shalla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5698-2678","authenticated-orcid":false,"given":"karima Akool","family":"AlSalihi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"22436","published-online":{"date-parts":[[2026,7,30]]},"container-title":["Wiadomo\u015bci Lekarskie"],"original-title":[],"link":[{"URL":"https:\/\/www.wiadomoscilekarskie.pl\/pdf-220739-145228","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.wiadomoscilekarskie.pl\/pdf-220739-145228","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T09:39:40Z","timestamp":1789551580000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.wiadomoscilekarskie.pl\/Molecular-Profiling-of-Apoptotic-Inflammatory-and-Angiogenic-Markers-in-Prostate,220739,0,2.html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,30]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026]]}},"alternative-id":["220739"],"URL":"https:\/\/doi.org\/10.36740\/wlek\/220739","relation":{},"ISSN":["0043-5147","2719-342X"],"issn-type":[{"value":"0043-5147","type":"print"},{"value":"2719-342X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,30]]}}}