{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T13:12:48Z","timestamp":1759497168510},"reference-count":31,"publisher":"ALUNA","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Wiad Lek"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:p>The aim: The goal of this study is to identify Uropathogenic E. coli multidrug-resistant bacteria, as well as their genetic profile clade and correlation with dispersion integron. \nMaterials and methods: Five hundred urine samples from UTI patients were collected in Al-Diwaniyah, Iraq. They were then investigated by a qualified consulting doctor. After cultivation in special media (MacConkey agar and Eosin-Methylene blue) to detect Enterobacteriacea, including Escherichia coli, the samples were identified using the Vitec 2 compact system, as well as MIC susceptibility testing to Amikacin, Levofloxacin, Cefepime, Meropenem, Nitrofurantion, and Trimethoprim-sulfamethoxazole for detect multidrug resistance isolates. Multiplex PCR was used to detect three types of integrase gene. Finally, ERIC2-PCR was used to detect the genetic profile of all isolates. \nResults: From 500 UTI samples, 22 isolates UPEC detected resistance to different class of antibiotic, including: 86.3% to Cefepime, 54.5% - to Trimethoprim-sulfamethoxazole, 31.8% -Levofloxacin, 18.8% - Amikacin, 18.8% - Imipenem, 0% to Nitrofurantion. Twenty of the 22 isolates had various integrase gene classes as: 54.5%, 36.3%, 72.2% for Intl I, Intl II, and Intl III, respectively; while two isolates have no integron. \nConclusions: Integron III has a higher incidence and compensates other classes; isolates with triple classes are more virulent and antibiotic resistant. Their genomic profile reveals association with human urine and unique clade of relatives.<\/jats:p>","DOI":"10.36740\/wlek202205205","type":"journal-article","created":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T15:37:08Z","timestamp":1656344228000},"page":"1254-1260","source":"Crossref","is-referenced-by-count":3,"title":["DISTRIBUTION OF INTEGRON III AND PHYLOGENIC CLADE AMONG MDR UROPATHOGENIC E. COLI FROM PATIENT IN AL-DIWANIYAH CITY, IRAQ"],"prefix":"10.36740","volume":"75","author":[{"given":"Ibtisam H.","family":"Al-Azawi","sequence":"first","affiliation":[{"name":"UNIVERSITY OF AL-QADISIYAH, DIWANIYA, IRAQ"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahasin S.","family":"Al-Bidiri","sequence":"additional","affiliation":[{"name":"BABYLON UNIVERSITY, BABYLON, IRAQ"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"22436","published-online":{"date-parts":[[2022]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"1. Malik S., Sidhu P.K., Rana J.S., Nehra K. Managing urinary tract infections through phage therapy: a novel approach. Folia Microbiol. Praha. 2020; 65(2): 217-231, doi: 10.1007\/s12223-019-00750-y.","DOI":"10.1007\/s12223-019-00750-y"},{"key":"ref1","doi-asserted-by":"publisher","unstructured":"2. 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J. 2016; 1(1): 1-7. doi: 10.36876\/smtmj.1003.","DOI":"10.36876\/smtmj.1003"}],"container-title":["Wiadomo\u015bci Lekarskie"],"original-title":[],"language":"en","deposited":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T15:37:22Z","timestamp":1656344242000},"score":1,"resource":{"primary":{"URL":"https:\/\/wiadlek.pl\/wp-content\/uploads\/archive\/2022\/05\/WLek202205205.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"references-count":31,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022]]},"published-print":{"date-parts":[[2022,5]]}},"URL":"https:\/\/doi.org\/10.36740\/wlek202205205","archive":["Internet Archive"],"relation":{},"ISSN":["0043-5147"],"issn-type":[{"value":"0043-5147","type":"print"}],"subject":[],"published":{"date-parts":[[2022]]}}}