{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T16:48:32Z","timestamp":1774889312888,"version":"3.50.1"},"reference-count":29,"publisher":"ALUNA","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Wiad Lek"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:p>The aim: The aim of the study is to experimentally test the process of viral infection and determine the ways of its penetration into the human body. \nMaterials and methods: This experimental analysis is based on systematic research, published peer-reviewed articles, books, textbooks, monographs. It should also be noted that in order to identify some immunocompetent lymph node cells and the ability to visualize certain sites in the lymphoid nodes of Peyer\u2019s patches, where the initial processes are presented below, we resorted to sampling anatomical material. The study involved 10 adult albino rats weighing 200.0 \u00b1 20.0 g. The search period covered the period from 2010 to 2021, but the experimental analysis contains some valuable data from previous years, as these literature sources have significant scientific value. \nResults: According to immunohistochemical analysis of the epithelium associated with the dome of the lymph nodes of the small intestine of white rats, the bulk was B-lymphocytes (about 47%) and T-lymphocytes (about 35%), while plasma cells, macrophages and dendritic cells accounted for approximately 5% for each of them. \nConclusions: \u0420rocess of development of viral infection can be represented in the form of the following targeted steps: 1) massive invasion of viruses into the body; 2) the pathway of viruses to the intended target (target cells) is carried out by the blood flow; 3) \u0430chieving the target by viruses and their penetration into target cells. \u0406n the pathogenesis of viral diseases, the role is played by the preparedness of the particular body, which directly depends on the functional state of its immune system, which determines the possibility, severity and outcome of the disease.<\/jats:p>","DOI":"10.36740\/wlek202206121","type":"journal-article","created":{"date-parts":[[2022,7,16]],"date-time":"2022-07-16T13:28:47Z","timestamp":1657978127000},"page":"1544-1549","source":"Crossref","is-referenced-by-count":6,"title":["EXPERIMENTAL ANALYSIS OF WAYS OF VIRAL INFECTIONS INTO THE HUMAN BODY"],"prefix":"10.36740","volume":"75","author":[{"given":"Volodymyr","family":"Hryn","sequence":"first","affiliation":[{"name":"POLTAVA STATE MEDICAL UNIVERSITY, POLTAVA, UKRAINE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yurij","family":"Kostylenko","sequence":"additional","affiliation":[{"name":"POLTAVA STATE MEDICAL UNIVERSITY, POLTAVA, UKRAINE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viktoriia","family":"Pinchuk","sequence":"additional","affiliation":[{"name":"POLTAVA STATE MEDICAL UNIVERSITY, POLTAVA, UKRAINE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valeriy","family":"Zhamardiy","sequence":"additional","affiliation":[{"name":"POLTAVA STATE MEDICAL UNIVERSITY, POLTAVA, UKRAINE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viktoriia","family":"Donchenko","sequence":"additional","affiliation":[{"name":"POLTAVA STATE MEDICAL UNIVERSITY, POLTAVA, UKRAINE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olha","family":"Honchar","sequence":"additional","affiliation":[{"name":"POLTAVA STATE MEDICAL UNIVERSITY, POLTAVA, UKRAINE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oksana","family":"Hordiienko","sequence":"additional","affiliation":[{"name":"NATIONAL UNIVERSITY \u00abYURI KONDRATYUK POLTAVA POLYTECHNIC\u00bb, POLTAVA, UKRAINE"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"22436","published-online":{"date-parts":[[2022]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"1. Al-Qahtani A.A. Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): Emergence, history, basic and clinical aspects. Saudi J Biol Sci. 2020;10:2531-2538. doi: 10.1016\/j.sjbs.2020.04.033.","DOI":"10.1016\/j.sjbs.2020.04.033"},{"key":"ref1","doi-asserted-by":"publisher","unstructured":"2. Lai C.C., Shih T.P., Ko W.C. et al. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and coronavirus disease-2019 (COVID-19) : The epidemic and the challenges. Int J Antimicrob Agents. 2020;55(3):105924. doi:10.1016\/j.ijantimicag.2020.105924.","DOI":"10.1016\/j.ijantimicag.2020.105924"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"3. Chen Y., Guo D. Molecular mechanisms of coronavirus RNA capping and methylation. Virol Sin. 2016;31(3):3\u201311. doi:10.1007\/s12250-016- 3726-4.","DOI":"10.1007\/s12250-016-3726-4"},{"key":"ref3","unstructured":"4. Petrovsky B. V. Great medical encyclopedia. M.: Soviet encyclopedia. 1976; 4:232-245."},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"5. Nikiforov V.V., Suranova T.G., Chernobrovkina T.Y. et al. The new coronavirus infection (Covid-19): clinical and epidemiological aspects. Archives of Internal Medicine. 2020;10 (52):87-93.","DOI":"10.20514\/2226-6704-2020-10-2-87-93"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"6. Mittal A., Manjunath K., Ranjan R.K. et al. COVID-19 pandemic : Insights into structure, function, and hACE2 receptor recognition by SARS-CoV-2. PLoS Pathog. 2020;16(8):e1008762. doi:10.1371\/journal.ppat.1008762.","DOI":"10.1371\/journal.ppat.1008762"},{"key":"ref6","unstructured":"7. Alberts B., Johnson A., Lewis J. Molecular Biology of the Cell. 4th edition. New York: Garland Science. Cell Biology of Infection. 2002. https:\/\/www. ncbi.nlm.nih.gov\/books\/NBK26833\/ [date access 08.01.2022]"},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"8. Boldogh I., Albrecht T., Porter D. Persistent Viral Infections. In: Baron S, editor. Medical Microbiology. 4th edition. Galveston (TX): University of Texas Medical Branch at Galveston. 1996. https:\/\/www.ncbi.nlm.nih. gov\/books\/NBK8538\/ [date access 08.01.2022]","DOI":"10.18600\/toj.010101"},{"key":"ref8","unstructured":"9. Shamray S.M., Leont\u2019yev D.V. Virusolohiia: pidruchnyk [Virology : a textbook]. Kharkiv National Pedagogical University named H. S. Skovorody. Kharkiv. 2019, 14p. (in Ukrainian)"},{"key":"ref9","doi-asserted-by":"publisher","unstructured":"10. Belouzard S., Millet J.\u041a., Beth N.L. et al. Mechanisms of Coronavirus Cell Entry Mediated by the Viral Spike Protein. Viruses. 2012;4:1011-1033. doi:10.3390\/v4061011.","DOI":"10.3390\/v4061011"},{"key":"ref10","doi-asserted-by":"publisher","unstructured":"11. Thorley J.A., Mc Keating J.A., Rappoport J.Z. Mechanisms of viral entry: sneaking in the front door. Protoplasma. 2010;244(1):15-24. doi: 10.1007\/s00709-010-0152-6.","DOI":"10.1007\/s00709-010-0152-6"},{"key":"ref11","unstructured":"12. Kolokol\u2019tsova T.D., Saburina I.N., Kubatiyev A.A. Human and animal cell cultures: isolation, cultivation, cryopreservation and control. Pathogenesis. 2015;13(2):50-65."},{"key":"ref12","unstructured":"13. Bezshapochnyy S., Gasyuk Yu., Loburets V. et al. Mechanisms of local protection of the mucous membrane of the nasal cavity and paranasal sinuses. Bulletin of otorhinolaryngology. 2013;78(4):44-47."},{"key":"ref13","doi-asserted-by":"publisher","unstructured":"14. Kurbacheva O.M., Amanturliyeva M.E. The role of the barrier function of the mucous membranes in allergic diseases and in sublingual allergen-specific immunotherapy. Bulletin of Siberian Medicine. 2017;16(2):32- 46. doi:10.20538\/1682-0363-2017-2-32\u201346.","DOI":"10.20538\/1682-0363-2017-2-32-46"},{"key":"ref14","doi-asserted-by":"publisher","unstructured":"15. Bustamante-Marin X.M., Ostrowski L.E. Cilia and Mucociliary Clearance. Cold Spring Harb Perspect Biol. 2017;9(4):a028241. doi:10.1101\/cshperspect.a028241.","DOI":"10.1101\/cshperspect.a028241"},{"key":"ref15","doi-asserted-by":"publisher","unstructured":"16. Le Messurier K., Tiwary M., Morin N., Samarasinghe A. Respiratory Barrier as a Safeguard and Regulator of Defense Against Influenza A Virus and Streptococcus pneumoniae. Front Immunol. 2020;11:3. doi:10.3389\/fimmu.2020.00003.","DOI":"10.3389\/fimmu.2020.00003"},{"key":"ref16","unstructured":"17. Kaydashev I.P., Shinkevich V.I. Modern aspects of the study of mucosal immunity. Dental technologists. 2006;1-2:18-21."},{"key":"ref17","unstructured":"18. Rabson A., Royt A., Delvz P. Fundamentals of Medical Immunology. Publ.: World: 2006, 320p."},{"key":"ref18","unstructured":"19. Galimzyanov K.M., Bashkina O.A., Dosmukhanova E.G. et al. Clinical significance of biofilm formation in bacteria. Astrakhan Medical Journal. 2018;13(4):32-42. doi: 10.17021\/2018.13.4.32.42."},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"20. Dobrodeyeva L.K., Samodova A.V., Patrakeyeva V.P. The ratio of microflora and innate immunity reactions in mucous-associated lymphoid tissue. Journal of Biomedical Research. 2015;2:71-80.","DOI":"10.17238\/issn2308-3174.2015.2.71"},{"key":"ref20","unstructured":"21. Okulich V.K., Kabanova A.A., Plotnikov F.V. Microbial biofilms in clinical microbiology and antibacterial therapy. Vitebsk: VSMU. 2017, 300p."},{"key":"ref21","unstructured":"22. Vershyhora A.Y., Paster E.U., Kolybo D.V. Immunology. For the general ed. EU Paster. K: High school. 2005, 599p."},{"key":"ref22","doi-asserted-by":"publisher","unstructured":"23. Hryn V. H. The general principle of the structure of lymphoid nodules in the composition of Peyer\u2019s patches of the small intestine of white rats. Bulletin of problems of biology and medicine. 2019;2(2):200-204. doi 10.29254\/2077-4214-2019-2-2-151-200-204.","DOI":"10.29254\/2077-4214-2019-2-2-151-200-204"},{"key":"ref23","doi-asserted-by":"publisher","unstructured":"24. Hryn V. Internal structure of the lymphoid nodules of the peyer\u2019s patches of small intestine in albino rats. Georgian Med News. 2019;296:122-126.","DOI":"10.31718\/mep.2020.24.3-4.05"},{"key":"ref24","doi-asserted-by":"publisher","unstructured":"25. Hryn V.H., Kostylenko Y.P. The structure of the lymphoid-associated epithelium of Peyer\u2019s patches of the small intestine of white rats. Clinical anatomy and operative surgery. 2019;18(4):67-75. doi: 10.24061\/1727-0847.18.4.2019.11.","DOI":"10.24061\/1727-0847.18.4.2019.11"},{"key":"ref25","doi-asserted-by":"publisher","unstructured":"26. Hryn V., Kostylenko Y., Korchan N. et al. Structural form of the follicle-associated epithelium of peyers\u2019 patches of the albino rats\u2019 small intestine. Georgian medical news. 2019;9(294):118-123.","DOI":"10.24061\/1727-0847.18.4.2019.11"},{"key":"ref26","doi-asserted-by":"publisher","unstructured":"27. Bykov A.S., Karaulov A.V., Tsomartova D.A. et al. M-cells are one of the important components in the initiation of an immune response in the intestine. Infection and immunity. 2018;8(3):263-272. doi: 10.15789\/2220-7619-2018-3-263-272.","DOI":"10.15789\/2220-7619-2018-3-263-272"},{"key":"ref27","unstructured":"28. Lashkevich V.A. The history of the creation in 1959 of a live vaccine from the attenuated strains of A. Sabin and the idea of eradicating poliomyelitis. Questions of Virology. 2013;58(1):4-10."},{"key":"ref28","doi-asserted-by":"publisher","unstructured":"29. Chumakov K.M., Ishmukhametov A.A. Polio vaccines: present and future. 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