{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T06:12:08Z","timestamp":1668492728682},"reference-count":15,"publisher":"ALUNA","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Wiad Lek"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:p>The aim: To study changes of the expression of synaptophysin (Syn) and vascular endothelial growth factor (VEGF) in neurons of the sensorimotor cortex (SMC) to reveal after unilateral ligation of the carotid artery, sensitization with brain antigen and their combination. \nMaterials and methods: Experimental animals \u2013 Wistar rats (260-290 g). Experimental models: mobilization of the left common carotid artery, ligation of the indicated artery, sensitization with cerebral antigen, combination of sensitization with cerebral antigen and ligation of the carotid artery. Methods: immunohistochemistry, quantitative densitometric assessment. \nResults: Dyscirculatory disorders of cerebral blood supply during unilateral mobilization or ligation of the common carotid artery, sensitization with cerebral antigen lead in rats to a transient decrease in synaptophysin expression and phase changes in VEGF expression in the SMC from the lesion side. These changes occur in the absence of morphological changes in the cerebral cortex. \nConclusions: The absence of morphological changes in the SMC in the short term (10-30 days) after minor trauma to the common carotid artery (separation from the bed and n.vagus) or its ligation is accompanied by a transient decrease in Syn expression and some increase in VEGF, which may reflect a violation of synaptic function and the general metabolic activity of neurons. Sensitization with a brain antigen, leading to an increase in the level of anti-brain antibodies and immune complexes in the blood of rats, can act as an independent damaging factor for the brain, and also potentiates and prolongs changes caused by impaired blood circulation.<\/jats:p>","DOI":"10.36740\/wlek202209214","type":"journal-article","created":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T20:28:19Z","timestamp":1668457699000},"page":"2256-2261","source":"Crossref","is-referenced-by-count":0,"title":["EXPRESSION OF SYNAPTOFYSIN AND VEGF IN THE SENSOMOTOR CORTEX DURING THE CAROTID ARTERY LIGATION, THE BRAIN ANTIGEN SENSITIZATION AND THEIR COMBINATIONS"],"prefix":"10.36740","volume":"75","author":[{"given":"Alexandr","family":"Grabovoy","sequence":"first","affiliation":[{"name":"BOGOMOLETS NATIONAL MEDICAL UNIVERSITY, KYIV, UKRAINE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilya","family":"Yaremenko","sequence":"additional","affiliation":[{"name":"BOGOMOLETS NATIONAL MEDICAL UNIVERSITY, KYIV, UKRAINE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergey","family":"Shepelev","sequence":"additional","affiliation":[{"name":"BOGOMOLETS NATIONAL MEDICAL UNIVERSITY, KYIV, UKRAINE"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"22436","published-online":{"date-parts":[[2022]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"1. de la Torre J.C. Are Major Dementias Triggered by Poor Blood Flow to the Brain? Theoretical Considerations. Journal of Alzheimer\u2019s disease: JAD. 2017; 57(2): 353-371.","DOI":"10.3233\/jad-161266"},{"key":"ref1","doi-asserted-by":"publisher","unstructured":"2. Choi B.R., Kim D.H., Back D.B. et a1. Characterization of white matter injury in a rat model of chronic cerebral hypoperfusion. Stroke. 2016; 47(2): 542-547.","DOI":"10.1161\/strokeaha.115.011679"},{"key":"ref2","doi-asserted-by":"publisher","unstructured":"3. Hei Y., Chen R., Yi X. et a1. HMGB1 neutralization attenuates hippocampal neuronal death and cognitive impairment in rats with chronic cerebral hypoperfusion via suppressing inflammatory responses and oxidative stress. Neuroscience. 2018; 383: 150-159.","DOI":"10.1016\/j.neuroscience.2018.05.010"},{"key":"ref3","doi-asserted-by":"publisher","unstructured":"4. Nishino A., Tajima Y., Takuwa H. et a1. Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model. Scientific reports. 2016; 6(1): 1-8.","DOI":"10.1038\/srep25072"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"5. Chen X., Jiang X.M., Zhao L.J. et a1. MicroRNA-195 prevents dendritic degeneration and neuron death in rats following chronic brain hypoperfusion. Cell death & disease. 2017; 8(6): e2850-e2850.","DOI":"10.1038\/cddis.2017.243"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"6. Sigfridsson E., Marangoni M., Johnson J.A. et a1. Astrocyte-specific overexpression of Nrf2 protects against optic tract damage and behavioural alterations in a mouse model of cerebral hypoperfusion. Scientific reports. 2018; 8(1): 1-14.","DOI":"10.1038\/s41598-018-30675-4"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"7. Manso Y., Holland P.R., Kitamura A. et a1. Minocycline reduces microgliosis and improves subcortical white matter function in a model of cerebral vascular disease. Glia. 2018; 66(1): 34-46.","DOI":"10.1002\/glia.23190"},{"key":"ref7","doi-asserted-by":"publisher","unstructured":"8. Diamond B., Honig G., Mader S. et al. Brain-reactive antibodies and disease. Annual review of immunology. 2013; 31: 345-385.","DOI":"10.1146\/annurev-immunol-020711-075041"},{"key":"ref8","unstructured":"9. Yaremenko L., Grabovoi A., Cherkasov V. et a1. Reactions of astrocytes and microglia of the sensorimotor cortex at ligation of the carotid artery, sensitization of the brain antigen and their combination. Georgian Med News. 2020; (304-305):122-127."},{"key":"ref9","unstructured":"10. Yaremenko L.M., Grabovy O.M., Bordonos V.G. The state of autoantibody titers to tissue antigens of the brain and circulating immune complexes in the modeling of blood supply disorders of the brain of varying severity and its correction, Immunology and Allergology (Kyiv). 2009; (2 \u2013 3): 55 \u2013 59."},{"key":"ref10","unstructured":"11. Bai X., Strong R. Expression of synaptophysin protein in different dopaminergic cell lines. Journal of biochemical and pharmacological research. 2014; 2(4): 185\u2013190."},{"key":"ref11","doi-asserted-by":"publisher","unstructured":"12. Licht T., Keshet E. Delineating multiple functions of VEGF-A in the adult brain. Cellular and Molecular Life Sciences. 2013; 70(10): 1727-1737.","DOI":"10.1007\/s00018-013-1280-x"},{"key":"ref12","doi-asserted-by":"publisher","unstructured":"13. Theis V., Theiss C. VEGF-A Stimulus for Neuronal Development and Regeneration in the CNS and PNS. Current Protein and Peptide Science. 2018; 19(6): 589-597.","DOI":"10.2174\/1389203719666180104113937"},{"key":"ref13","doi-asserted-by":"publisher","unstructured":"14. Cosky E.E.P., Ding Y. The role of vascular endothelial growth factor in angiogenesis and brain circulation after stroke. Brain circ. 2018; 4(2): 73-75.","DOI":"10.4103\/bc.bc_8_18"},{"key":"ref14","doi-asserted-by":"publisher","unstructured":"15. Yu W., Jin H., Sun W. et a1. Connexin 43 promotes angiogenesis through activating the HIF-1\u03b1\/VEGF signaling pathway under chronic cerebral hypoperfusion. J Cereb Blood Flow Metab. 2021; 41(10): 2656-2675.","DOI":"10.1177\/0271678x211010354"}],"container-title":["Wiadomo\u015bci Lekarskie"],"original-title":[],"language":"en","deposited":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T20:28:33Z","timestamp":1668457713000},"score":1,"resource":{"primary":{"URL":"https:\/\/wiadlek.pl\/wp-content\/uploads\/archive\/2022\/09\/WLek202209214.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"references-count":15,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2022]]},"published-print":{"date-parts":[[2022,9]]}},"URL":"https:\/\/doi.org\/10.36740\/wlek202209214","archive":["Internet Archive"],"relation":{},"ISSN":["0043-5147"],"issn-type":[{"value":"0043-5147","type":"print"}],"subject":[],"published":{"date-parts":[[2022]]}}}