{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T12:14:49Z","timestamp":1778328889838,"version":"3.51.4"},"reference-count":0,"publisher":"Politechnika Wroclawska Oficyna Wydawnicza","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Bioeng Biomech"],"published-print":{"date-parts":[[2024]]},"abstract":"<jats:p>Purpose<\/jats:p>\n\t\t\t\t\t<jats:p>The aim of this study was to investigate the effect of substrate - polycaprolactone (PCL) based porous membrane modified with rosmarinic acid (RA), (PCL-RA) and to determine the optimal values of low field laser irradiation (LLLT) as stimulators of biological response of RAW 264.7 macrophages.<\/jats:p>\n\t\t\t\t\t<jats:p>Methods<\/jats:p>\n\t\t\t\t\t<jats:p>The porous polymer membrane was obtained by the phase inversion method, the addition of rosmarinic acid was 1%wt. The reference material was pure polymer membrane. RAW 264.7 were deposited on the material and then irradiated with a laser with a wavelength of 808 nm, a power of 100 mW, an irradiation dose of 2 J\/cm2\/cell well, applied continuously (C), (100\/2\/C) or pulsed (I), (100\/2\/I).<\/jats:p>\n\t\t\t\t\t<jats:p>Results<\/jats:p>\n\t\t\t\t\t<jats:p>Macrophage irradiation resulted in an increase their adhesion. Modifying the PCL membranes with rosmarinic acid had no effect on cell viability on day 3 of the cell culture. Irradiation of macrophages cultured on PCL-RA material increased their viability. Irradiation of macrophages cultured on PCL-RA material decreased macrophage secretion of NO and protein and the increase in TNF and MCP-1 secretion was only transient on day 3 of culture.<\/jats:p>\n\t\t\t\t\t<jats:p>Conclusions<\/jats:p>\n\t\t\t\t\t<jats:p>Macrophage irradiation had a positive effect on macrophage attachment. Modification of PCL membranes with rosmarinic acid influenced the biological activity of macrophages. Culture of macrophages on rosmarinic acid-modified PCL membranes and simultaneous irradiation of LLLT cells resulted in anti-inflammatory effects.<\/jats:p>","DOI":"10.37190\/abb-02451-2024-02","type":"journal-article","created":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T13:12:01Z","timestamp":1726492321000},"source":"Crossref","is-referenced-by-count":2,"title":["Macrophage activity modulation via synergistic effect of a porous substrate and low-field laser therapy"],"prefix":"10.37190","volume":"26","author":[{"given":"Anna","family":"\u015acis\u0142owska-Czarnecka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandra","family":"Matu\u0142a","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amelia","family":"Lizak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa","family":"Stodola-Zych","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beata","family":"Stenka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna","family":"Homa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aneta","family":"Bac","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aneta","family":"Teleg\u0142\u00f3w","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"23140","container-title":["Acta of Bioengineering and Biomechanics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/actabio.pwr.edu.pl\/fcp\/PGBUKOQtTKlQhbx08SlkTUARAUWRuHQwFDBoIVURNWHxTAFhnRlUuWTISTnoYDxMe\/302\/public\/publikacje\/v26-2-2024\/36.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T12:53:29Z","timestamp":1736513609000},"score":1,"resource":{"primary":{"URL":"https:\/\/actabio.pwr.edu.pl\/fcp\/PGBUKOQtTKlQhbx08SlkTUARAUWRuHQwFDBoIVURNWHxTAFhnRlUuWTISTnoYDxMe\/302\/public\/publikacje\/v26-2-2024\/36.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":0,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.37190\/abb-02451-2024-02","relation":{},"ISSN":["1509-409X","2450-6303"],"issn-type":[{"value":"1509-409X","type":"print"},{"value":"2450-6303","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024]]}}}