{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T11:32:43Z","timestamp":1747222363285,"version":"3.40.5"},"reference-count":0,"publisher":"Sociedad Espanola de Quimioterapia","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Esp Quimioter"],"published-print":{"date-parts":[[2023,11,27]]},"abstract":"<jats:p>Background. The prevalence of drug-resistant Neisseria gonorrhoeae (NG) infections is increasing. Studies report the prevalence of NG strains presenting A2059G\/C2611T (rRNA 23S) and S91F (parC) mutations conferring resistance to azithromycin and ciprofloxacin. Material and methods. We conducted a prospective cohort study evaluating first void-urine urines, rectal, and oropharyngeal swabs collected from a cohort of patients in a tertiary hospital in Madrid between October 2022 and January 2023. Samples were screened by Allplex\u2122 7-STI Essential Assay (Seegene\u00ae). Drug resistances were performed by Allplex\u2122 NG&amp;DR Assay (Seegene\u00ae). Results. A total of 1,415 patients were included, of which 112 had a positive sample for NG infection. One patient had a C2611T mutation (0.9%) and neither patient showed A2059G mutation. We found 67 (59.8%) S91F-positive patients. Forty-four patients (39.3%) not had any mutations. Conclusions. We report a low-prevalence of mutations A2059G\/C2611T to macrolides and a high-prevalence to S91F in NG infections. Molecular methods for the detection of NG resistance could be useful in direct non-culturable samples.<\/jats:p>","DOI":"10.37201\/req\/058.2023","type":"journal-article","created":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T14:14:40Z","timestamp":1695046480000},"page":"625-628","source":"Crossref","is-referenced-by-count":2,"title":["Prevalence of mutations associated with macrolide and fluoroquinolone resistance in Neisseria gonorrhoeae with Allplex\u2122 NG&amp;DR Assay (Seegene\u00ae) in a tertiary hospital from Madrid, Spain"],"prefix":"10.37201","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1856-1725","authenticated-orcid":false,"given":"Alfredo","family":"Maldonado-Barrueco","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Sanz-Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5888-7706","authenticated-orcid":false,"given":"Iker","family":"Falces-Romero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paloma","family":"Garc\u00eda-Clemente","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5888-7706","authenticated-orcid":false,"given":"Juana","family":"Cacho-Calvo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5888-7706","authenticated-orcid":false,"given":"Inmaculada","family":"Quiles-Melero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"23328","published-online":{"date-parts":[[2023,9,18]]},"container-title":["Revista Espa\u00f1ola de Quimioterapia"],"original-title":[],"deposited":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T09:35:06Z","timestamp":1701336906000},"score":1,"resource":{"primary":{"URL":"https:\/\/seq.es\/abstract\/rev-esp-quimioter-2023-september-18-2\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,18]]},"references-count":0,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,11,27]]},"published-print":{"date-parts":[[2023,11,27]]}},"URL":"https:\/\/doi.org\/10.37201\/req\/058.2023","relation":{},"ISSN":["0214-3429","1988-9518"],"issn-type":[{"type":"print","value":"0214-3429"},{"type":"electronic","value":"1988-9518"}],"subject":[],"published":{"date-parts":[[2023,9,18]]}}}