{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T13:56:56Z","timestamp":1774533416425,"version":"3.50.1"},"reference-count":0,"publisher":"Sociedad Espanola de Quimioterapia","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Esp Quimioter"],"published-print":{"date-parts":[[2023,1,20]]},"abstract":"<jats:p>Introduction. Microscopic examination of the intestinal parasites, from the patient\u2019s concentrated feces, has a lower sensitivity when compared to molecular diagnostic techniques. Therefore, the objective of this study has been to compare both techniques, as well as to evaluate whether there is a correlation between the microscopic examination and the threshold cycles (Ct) obtained for Blastocystis hominis. Material and methods. Retrospective study of the samples received in the Microbiology laboratory during September 2021. The MiniParasep SF\u00ae concentration test was performed for microscopic visualization and then PCR was performed with the Seegene AllplexTM Parasite Assay panel. Results. A 27% (n=74) of the samples were positive by molecular diagnosis, with a total of 87 parasites detected. 53% (n=39) were women with a mean age of 47 \u00b1 24 years. In 76% (n=56) of the cases the service of origin was Primary Care. The most frequently found parasite was B. hominis, 85% (n=64), followed by Dientamoeba fragilis 20% (n=15) and Giardia lamblia 11% (n=8). Co-infection by two parasites was detected in 13 cases (B. hominis + D. fragilis in 6 cases, and B. hominis + G. lamblia in 7 cases). In the microscopic diagnosis, 9.5% (n=26) positivity was obtained. The most frequently found parasite was B. hominis, 84% (n=23), followed by G. lamblia, which was seen in three cases by microscopy. D. fragilis was not seen in any case. Coinfection of B. hominis + G. lamblia was observed in one sample. Conclusions. Techniques for molecular diagnosis of intestinal parasites are fast, reliable and more sensitive than microscopic techniques, improving microbiological diagnosis and quality of care.<\/jats:p>","DOI":"10.37201\/req\/088.2022","type":"journal-article","created":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T18:08:42Z","timestamp":1669918122000},"page":"88-91","source":"Crossref","is-referenced-by-count":5,"title":["Microscopy vs. molecular biology in the diagnosis of intestinal protozoal infections, is it time for a change?"],"prefix":"10.37201","volume":"36","author":[{"given":"Laura","family":"Sante Fern\u00e1ndez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Cap\u00f3n Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Moreno Flores","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Coira Mar\u00edn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pilar","family":"Alonso Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"23328","published-online":{"date-parts":[[2022,12,1]]},"container-title":["Revista Espa\u00f1ola de Quimioterapia"],"original-title":["Microscop\u00eda vs. biolog\u00eda molecular en el diagn\u00f3stico de infecciones protozoarias intestinales, \u00bfes el momento del cambio?"],"deposited":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T13:52:19Z","timestamp":1674222739000},"score":1,"resource":{"primary":{"URL":"https:\/\/seq.es\/abstract\/rev-esp-quimioter-2022-december-1-2\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,1]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,1,20]]},"published-print":{"date-parts":[[2023,1,20]]}},"URL":"https:\/\/doi.org\/10.37201\/req\/088.2022","relation":{},"ISSN":["0214-3429","1988-9518"],"issn-type":[{"value":"0214-3429","type":"print"},{"value":"1988-9518","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,1]]}}}