{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T17:47:17Z","timestamp":1776793637792,"version":"3.51.2"},"reference-count":0,"publisher":"Sociedad Espanola de Quimioterapia","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rev Esp Quimioter"],"published-print":{"date-parts":[[2021,11,24]]},"abstract":"<jats:p>Objetive. The aim of this study was to analyze the activity of the imipenem-relebactam combination (IMI\/REL) against a collection of multidrug-resist Enterobacterales, Pseudomonas aeruginosa and Acinetobacter baumannii clinical isolates. Material and methods. The study was conducted in two tertiary hospitals in Spain and included 192 clinical isolates of these 3 genera (139 resistant and 53 susceptible to IMI). The MICs for IMI with and without REL (at a fixed concentration of 4 mg\/L) were determined by a standard broth microdilution method according to international recommendations. Results. All IMI-susceptible E. coli strains were also susceptible to IMI\/REL. Enterobacterales resistant to IMI due to the production of carbapenemases, the MIC50 and MIC90 decreased from 64\/256 with IMI to 8\/64 mg\/L with IMI\/REL. This high activity was principally detected among isolates with KPC enzymes. Enterobacterales with class B carbapenemases, P. aeruginosa carrying VIM carbapenemase and A. baumannii strains showed no changes on IMI MIC50 or MIC90 after adding REL. Among P. aeruginosa strains without carbapenemase the MIC for IMI\/REL was reduced between 1 to 5 dilutions. Conclusions. IMI\/REL showed high activity against the strains that carry Klebsiella pneumoniae carbapenemase (KPC) and against carbapenem-resistant P. aeruginosa unrelated to the VIM enzyme, mainly AmpC beta lactamase associated with impermeability. Against strains carrying oxacillinase 48 (OXA-48) associated with extended-spectrum beta-lactamase (ESBL), IMI\/REL presented activity only slightly better than IMI and had no beneficial effect superior to IMI against A. baumannii.<\/jats:p>","DOI":"10.37201\/req\/102.2021","type":"journal-article","created":{"date-parts":[[2021,10,12]],"date-time":"2021-10-12T05:59:46Z","timestamp":1634018386000},"page":"668-671","source":"Crossref","is-referenced-by-count":8,"title":["In vitro activity of imipenem\/relebactam against Gram-negative clinical isolates in two Spanish tertiary hospitals"],"prefix":"10.37201","volume":"34","author":[{"given":"Marina","family":"Pe\u00f1uelas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Garc\u00eda-Salguero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melania","family":"I\u00f1igo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose Manuel","family":"Vi\u00f1uela-Prieto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco Javier","family":"Candel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Luis","family":"del Pozo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esther","family":"Culebras","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Clinical Microbiology, Hospital Cl\u00ednico San Carlos, IdISCC and IML Health Research Institutes, Complutense University, Madrid, Spain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"23328","published-online":{"date-parts":[[2021,10,13]]},"container-title":["Revista Espa\u00f1ola de Quimioterapia"],"original-title":[],"deposited":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T06:32:49Z","timestamp":1637735569000},"score":1,"resource":{"primary":{"URL":"https:\/\/seq.es\/abstract\/rev-esp-quimioter-2021-october-13-4\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,13]]},"references-count":0,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,11,24]]},"published-print":{"date-parts":[[2021,11]]}},"URL":"https:\/\/doi.org\/10.37201\/req\/102.2021","relation":{},"ISSN":["0214-3429","1988-9518"],"issn-type":[{"value":"0214-3429","type":"print"},{"value":"1988-9518","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,13]]}}}