{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:35:39Z","timestamp":1777509339629,"version":"3.51.4"},"reference-count":39,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:p>\n                    Transplantation of isolated hepatocytes has been proposed to compensate for essential functions lacking in liver failure or for genetic defects that alter a specific liver metabolic pathway. Hepatocyte utilization for these purposes would be facilitated with a reliable, reproducible, and effective method of long-term hepatocyte storage. We have recently developed a simple new system for cryopreservation of hepatocytes that encapsulates alginate microspheres and maintains liver-specific function. The aim of this study was to elucidate the transport and drug-metabolizing enzyme activities of cryopreserved microencapsulated hepatocytes stored for a long time. Morphological examinations showed there is no apparent injury of the hepatocytes during cryopreservation processes. A drug-metabolizing enzyme (testosterone 6\u03b2-hydroxylase, a specific probe for CYP3A2) and drug transport activities [salicylate, allopurinol, and prostaglandin E\n                    <jats:sub>2<\/jats:sub>\n                    (PGE\n                    <jats:sub>2<\/jats:sub>\n                    ), typical substrates of rOat2] in cryopreserved microencapsulated hepatocytes were maintained up to 120 days. Our results thus demonstrate for the first time that cryopreservation of primary rat hepatocytes by the encapsulation technique allows long-term retention of drug metabolism and drug transport activities.\n                  <\/jats:p>","DOI":"10.3727\/000000007783464489","type":"journal-article","created":{"date-parts":[[2014,9,17]],"date-time":"2014-09-17T21:52:25Z","timestamp":1410990745000},"page":"67-73","source":"Crossref","is-referenced-by-count":15,"title":["Long-Term Maintenance of the Drug Transport Activity in Cryopreservation of Microencapsulated Rat Hepatocytes"],"prefix":"10.1177","volume":"16","author":[{"given":"Tomotake","family":"Koizumi","sequence":"first","affiliation":[{"name":"Second Department of Surgery, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Aoki","sequence":"additional","affiliation":[{"name":"Second Department of Surgery, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuna","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Yasuda","sequence":"additional","affiliation":[{"name":"Second Department of Surgery, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiko","family":"Izumida","sequence":"additional","affiliation":[{"name":"Second Department of Surgery, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenghao","family":"Jin","sequence":"additional","affiliation":[{"name":"Second Department of Surgery, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobukazu","family":"Nishino","sequence":"additional","affiliation":[{"name":"Second Department of Surgery, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinori","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Second Department of Surgery, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirohisa","family":"Kato","sequence":"additional","affiliation":[{"name":"Second Department of Surgery, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriyuki","family":"Murai","sequence":"additional","affiliation":[{"name":"Second Department of Surgery, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Niiya","sequence":"additional","affiliation":[{"name":"Second Department of Surgery, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuta","family":"Enami","sequence":"additional","affiliation":[{"name":"Second Department of Surgery, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keitaro","family":"Mitamura","sequence":"additional","affiliation":[{"name":"Second Department of Surgery, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshinori","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuo","family":"Kusano","sequence":"additional","affiliation":[{"name":"Second Department of Surgery, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,6,22]]},"reference":[{"key":"bibr1-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1177\/096368979500400607"},{"key":"bibr2-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199703270-00002"},{"key":"bibr3-000000007783464489","doi-asserted-by":"publisher","DOI":"10.3727\/000000003783985124"},{"key":"bibr4-000000007783464489","doi-asserted-by":"publisher","DOI":"10.3727\/000000005783982710"},{"key":"bibr5-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510280527"},{"key":"bibr6-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4804(90)90079-H"},{"key":"bibr7-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840100518"},{"key":"bibr8-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1016\/0011-2240(88)90040-5"},{"key":"bibr9-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1097\/01.sla.0000124298.74199.e5"},{"key":"bibr10-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1007\/BF01307530"},{"key":"bibr11-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199303000-00028"},{"key":"bibr12-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1016\/0011-2240(92)90048-7"},{"key":"bibr13-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1007\/s10157-005-0368-5"},{"key":"bibr14-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2004.04.009"},{"key":"bibr15-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199805143382004"},{"key":"bibr16-000000007783464489","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/S0031-6997(25)00021-3","volume":"40","author":"Gonzales F. J.","year":"1988","journal-title":"Pharmacol. Rev."},{"key":"bibr17-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1021\/bi00387a022"},{"key":"bibr18-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1006\/taap.1996.0299"},{"key":"bibr19-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2736(02)00633-8"},{"key":"bibr20-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.6180"},{"key":"bibr21-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1016\/0011-2240(90)90045-6"},{"key":"bibr22-000000007783464489","doi-asserted-by":"publisher","DOI":"10.3727\/000000003108747217"},{"key":"bibr23-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1016\/0887-2333(91)90046-G"},{"key":"bibr24-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"bibr25-000000007783464489","doi-asserted-by":"publisher","DOI":"10.3727\/000000003108747181"},{"key":"bibr26-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1126\/science.818706"},{"key":"bibr27-000000007783464489","first-page":"3052","volume":"24","author":"Mito M.","year":"1992","journal-title":"Transplant. Proc."},{"key":"bibr28-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2004.10.006"},{"key":"bibr29-000000007783464489","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1016\/S0022-3565(24)29490-8","volume":"298","author":"Morita N.","year":"2001","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"bibr30-000000007783464489","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1016\/S0090-9556(25)06846-1","volume":"15","author":"Powis G.","year":"1987","journal-title":"Drug Metab. Dispos."},{"key":"bibr31-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007690009927"},{"key":"bibr32-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-199405000-00012"},{"key":"bibr33-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(08)61797-5"},{"key":"bibr34-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)00585-7"},{"key":"bibr35-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1097\/01.sla.0000149303.48692.0f"},{"key":"bibr36-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199702270-00014"},{"key":"bibr37-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1594.1992.tb00336.x"},{"key":"bibr38-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(92)90014-A"},{"key":"bibr39-000000007783464489","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-199705000-00005"}],"container-title":["Cell Transplantation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.3727\/000000007783464489","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.3727\/000000007783464489","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:34:32Z","timestamp":1777390472000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.sagepub.com\/doi\/10.3727\/000000007783464489"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.3727\/000000007783464489"],"URL":"https:\/\/doi.org\/10.3727\/000000007783464489","relation":{},"ISSN":["0963-6897","1555-3892"],"issn-type":[{"value":"0963-6897","type":"print"},{"value":"1555-3892","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1]]}}}