{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:35:22Z","timestamp":1790760922642,"version":"4.1.0"},"reference-count":46,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2008,7,1]],"date-time":"2008-07-01T00:00:00Z","timestamp":1214870400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:p>Development of the urokinase plasminogen activator\/SCID (uPA\/SCID) transgenic mouse model has opened new perspectives for the study of different biological mechanisms such as liver regeneration, stem cell differentiation, and human hepatic pathogens. We observed that homozygous uPA\/SCID mice (uPA+\/+\/SCID) had a small offspring, indicating a fertility defect. The goal of this study was thus to rescue the fertility of homozygous uPA mice. A deregulation of ovarian function with an absence of corpus luteum was observed in female uPA+\/+\/SCID mice. In male uPA+\/+\/SCID mice, a decrease of the weight of the testes, epididymis, seminal vesicle, and prostate was measured. This was associated with an absence of seminal and prostatic secretions and a reduction in testicular sperm production. We hypothesized that the infertility of mice was the consequence of uPA-induced liver injury. Thus, in order to rescue liver function, hepatocytes from mice negative for the uPA transgene were transplanted into uPA+\/+\/SCID mice. Thirty days after cell transplantation, the livers of transplanted uPA+\/+\/SCID mice were totally repopulated and presented a normal morphology. Furthermore, transplantation restored normal body weight, life span, and reproductive organ function. In conclusion, we demonstrated that the transplantation of uPA+\/+\/SCID mice with healthy hepatocytes was sufficient to rescue the reproductive capacity of female and male uPA homozygous animals, highlighting the importance of normal liver function to reproductive capability.<\/jats:p>","DOI":"10.3727\/096368908786516800","type":"journal-article","created":{"date-parts":[[2008,11,20]],"date-time":"2008-11-20T19:50:17Z","timestamp":1227210617000},"page":"803-812","source":"Crossref","is-referenced-by-count":23,"title":["Rescue of Fertility in Homozygous Mice for the Urokinase Plasminogen Activator Transgene by the Transplantation of Mouse Hepatocytes"],"prefix":"10.1177","volume":"17","author":[{"given":"Nicolas M.","family":"Brezillon","sequence":"first","affiliation":[{"name":"INSERM, U845, 75015 Paris, France"},{"name":"Universit\u00e9 Paris Descartes, facult\u00e9 de m\u00e9decine Ren\u00e9 Descartes, CHU Necker, 75015 Paris, France"},{"name":"Institut Pasteur, D\u00e9partement de Virologie, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Dasilva","sequence":"additional","affiliation":[{"name":"INSERM, U845, 75015 Paris, France"},{"name":"Universit\u00e9 Paris Descartes, facult\u00e9 de m\u00e9decine Ren\u00e9 Descartes, CHU Necker, 75015 Paris, France"},{"name":"Institut Pasteur, D\u00e9partement de Virologie, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"L'h\u00f4te","sequence":"additional","affiliation":[{"name":"INSERM U567\/CNRS UMR8104, Universit\u00e9 Paris Descartes, facult\u00e9 de m\u00e9decine Ren\u00e9 Descartes, CHU Cochin, 75014 Paris, France"},{"name":"INRA, UMR 1061, Universit\u00e9 de Limoges, 87000 Limoges, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Bernex","sequence":"additional","affiliation":[{"name":"INRA, UMR 955, 94704 Maison-Alfort, France"},{"name":"Ecole Nationale V\u00e9t\u00e9rinaire d'Alfort, Service d'Anatomopathologie, 94704 Maisons-Alfort, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Piquet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Descartes, facult\u00e9 de m\u00e9decine Ren\u00e9 Descartes, CHU Necker, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Binart","sequence":"additional","affiliation":[{"name":"INSERM, U845, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serban","family":"Morosan","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Pierre et Marie Curie, Centre d'exp\u00e9rimentation fonctionnelle, 75005 Paris, France"},{"name":"Facultatea de Medicina Veterinara, 700490 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dina","family":"Kremsdorf","sequence":"additional","affiliation":[{"name":"INSERM, U845, 75015 Paris, France"},{"name":"Universit\u00e9 Paris Descartes, facult\u00e9 de m\u00e9decine Ren\u00e9 Descartes, CHU Necker, 75015 Paris, France"},{"name":"Facultatea de Medicina Veterinara, 700490 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2008,7,1]]},"reference":[{"key":"bibr1-096368908786516800","doi-asserted-by":"publisher","DOI":"10.1002\/lt.20111"},{"key":"bibr2-096368908786516800","doi-asserted-by":"publisher","DOI":"10.1002\/lt.21147"},{"key":"bibr3-096368908786516800","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63810-2"},{"key":"bibr4-096368908786516800","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mt.6300232"},{"issue":"1","key":"bibr5-096368908786516800","first-page":"73","volume":"1","author":"Bucuvalas J. 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