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The current study investigated the impact of graft distribution on the yield of DARPP-32-positive cells in the grafts following either single-tract or multitract cell delivery protocols using the microtransplantation approach. Cells derived from the whole ganglionic eminence of E15 rat embryos, ubiquitously expressing green fluorescent protein (GFP), were implanted into unilaterally QA-lesioned rat striatum either as 2 \u00d7 1.8 \u03bcl macrodeposits in a single tract, or as 18 \u00d7 0.2 \u03bcl microdeposits disseminated over six needle, multitract, penetrations. For both groups, an ultrathin glass capillary with an outer diameter of 50 \u03bcm was used. Histological assessment at 4 months after transplantation showed nearly twofold increase of DARRP-32-positive striatal-like neurons in the multitract compared to the single-tract group. However, the cellular make-up of the grafts did not translate into functional differences as tested in a basic spontaneous behavior test. Furthermore, the volumetric values for overall volume, DARPP-32-positive patches, and dopaminergic projection zones were similar between both groups. The results show that distribution of fetal striatal tissue in multiple submicroliter deposits provides for an increased yield of striatal-like neurons, potentially due to the enlargement of the graft\u2013host border area intensifying the graft's exposure to host-derived factors. Furthermore, the use of embryonic tissue from GFP donors was validated in cell-based therapy studies in the HD model.<\/jats:p>","DOI":"10.3727\/096368910x547435","type":"journal-article","created":{"date-parts":[[2011,12,9]],"date-time":"2011-12-09T14:58:31Z","timestamp":1323442711000},"page":"1515-1527","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Multitract Microtransplantation Increases the Yield of DARPP-32-Positive Embryonic Striatal Cells in a Rodent Model of Huntington's Disease"],"prefix":"10.1177","volume":"20","author":[{"given":"Wei","family":"Jiang","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Neurosurgery, Department of Stereotactic and Functional Neurosurgery, Neurocentre, Albert-Ludwigs-University of Freiburg, Freiburg, Germany"},{"name":"Department of Neurosurgery, Tongji Hospital of Tongji Medical College, Huazhong University of Science & Technology, Wuhan, 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