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Here, we used human immature dental pulp stem cells (hIDPSCs) as an alternative source for creating iPSC. hIDPSCs can be easily isolated from accessible tissue of young and adult patients. hIDPSCs possess a fibroblast-like morphology, retaining characteristics of adult multipotent stem cells. Reprogramming of hIDPSCs was fast, producing primary hIDPSC-iPSC colonies even under feeder-free conditions. hIDPSCs acquired ESC-like morphology, expressed pluripotent markers, possessed stable, normal karyotypes, and demonstrated the ability to differentiated in vitro and in vivo. Our data demonstrate that hIDPSCs-iPSCs offer an advantageous cell system for future cell therapy and basic studies, particularly as a model for pediatric developmental disorders.<\/jats:p>","DOI":"10.3727\/096368911x566235","type":"journal-article","created":{"date-parts":[[2011,12,9]],"date-time":"2011-12-09T15:31:23Z","timestamp":1323444683000},"page":"1707-1719","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":79,"title":["Feeder-Free Derivation of Induced Pluripotent Stem Cells from Human Immature Dental Pulp Stem Cells"],"prefix":"10.1177","volume":"20","author":[{"given":"Patr\u00edcia C. B.","family":"Beltr\u00e3o-Braga","sequence":"first","affiliation":[{"name":"School of Arts, Science and Humanities, Department of Obstetrics, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"},{"name":"Faculty of Veterinary Medicine, Department of Surgery and Department of Pathology, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"},{"name":"National Institute of Science and Technology in Stem Cell and Cell Therapy (INCTC), Ribeir\u00e3o Preto, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graciela C.","family":"Pignatari","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Surgery and Department of Pathology, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"},{"name":"National Institute of Science and Technology in Stem Cell and Cell Therapy (INCTC), Ribeir\u00e3o Preto, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo C.","family":"Maiorka","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Surgery and Department of Pathology, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00e9lio A. 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