{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T10:22:12Z","timestamp":1792059732501,"version":"4.4.0"},"reference-count":45,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p>\n                    Although transplantation of adult bone marrow mesenchymal stem cells (BM-MSCs) holds promise in the treatment for pulmonary arterial hypertension (PAH), the poor survival and differentiation potential of adult BM-MSCs have limited their therapeutic efficiency. Here, we compared the therapeutic efficacy of human embryonic stem cell-derived MSCs (hESC-MSCs) with adult BM-MSCs for the treatment of PAH in an animal model. One week following monocrotaline (MCT)-induced PAH, mice were randomly assigned to receive phosphate-buffered saline (MCT group); 3.0 \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    human BM-derived MSCs (BM-MSCs group) or 3.0 \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    hESC-derived MSCs (hESC-MSCs group) via tail vein injection. At 3 weeks posttransplantation, the right ventricular systolic pressure (RVSP), degree of RV hypertrophy, and medial wall thickening of pulmonary arteries were lower=, and pulmonary capillary density was higher in the hESC-MSC group as compared with BM-MSC and MCT groups (all p &lt; 0.05). At 1 week posttransplantation, the number of engrafted MSCs in the lungs was found significantly higher in the hESC-MSC group than in the BM-MSC group (all p &lt; 0.01). At 3 weeks posttransplantation, implanted BM-MSCs were undetectable whereas hESC-MSCs were not only engrafted in injured pulmonary arteries but had also undergone endothelial differentiation. In addition, protein profiling of hESC-MSC- and BM-MSC-conditioned medium revealed a differential paracrine capacity. Classification of these factors into bioprocesses revealed that secreted factors from hESC-MSCs were preferentially involved in early embryonic development and tissue differentiation, especially blood vessel morphogenesis. We concluded that improved cell survival and paracrine capacity of hESC-MSCs provide better therapeutic efficacy than BM-MSCs in the treatment for PAH.\n                  <\/jats:p>","DOI":"10.3727\/096368912x653020","type":"journal-article","created":{"date-parts":[[2012,12,4]],"date-time":"2012-12-04T03:21:31Z","timestamp":1354591291000},"page":"2225-2239","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":69,"title":["Improved Cell Survival and Paracrine Capacity of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells Promote Therapeutic Potential for Pulmonary Arterial Hypertension"],"prefix":"10.1177","volume":"21","author":[{"given":"Yuelin","family":"Zhang","sequence":"first","affiliation":[{"name":"Cardiology Division, Department of Medicine, University of Hong Kong, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songyan","family":"Liao","sequence":"additional","affiliation":[{"name":"Cardiology Division, Department of Medicine, University of Hong Kong, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mo","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, PR China"},{"name":"Department of Pharmacology and Pharmacy, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoting","family":"Liang","sequence":"additional","affiliation":[{"name":"Cardiology Division, Department of Medicine, University of Hong Kong, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Wai","family":"Poon","sequence":"additional","affiliation":[{"name":"Cardiology Division, Department of Medicine, University of Hong Kong, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chee-Yin","family":"Wong","sequence":"additional","affiliation":[{"name":"MAKNA Cancer Research Institute, Kuala Lumpur, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junwen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Hong Kong, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongjun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Hong Kong, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soon-Keng","family":"Cheong","sequence":"additional","affiliation":[{"name":"MAKNA Cancer Research Institute, Kuala Lumpur, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuen-Neng","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Surgery, National University Hospital, National University of Singapore, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hung-Fat","family":"Tse","sequence":"additional","affiliation":[{"name":"Cardiology Division, Department of Medicine, University of Hong Kong, Hong Kong"},{"name":"Research Centre of Heart, Brain, Hormone, and Healthy Aging, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qizhou","family":"Lian","sequence":"additional","affiliation":[{"name":"Cardiology Division, Department of Medicine, University of Hong Kong, Hong Kong"},{"name":"Research Centre of Heart, Brain, Hormone, and Healthy Aging, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong"},{"name":"Eye Institute, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2012,10,1]]},"reference":[{"issue":"11","key":"bibr1-096368912X653020","first-page":"I38","volume":"116","author":"Amsalem Y.","year":"2007","journal-title":"Circulation"},{"key":"bibr2-096368912X653020","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2009.08.092"},{"key":"bibr3-096368912X653020","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00173.2006"},{"key":"bibr4-096368912X653020","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2009.04.017"},{"key":"bibr5-096368912X653020","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","volume":"57","author":"Benjamini Y.","year":"1995","journal-title":"J. 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