{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:53:41Z","timestamp":1777510421547,"version":"3.51.4"},"reference-count":34,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:00:00Z","timestamp":1351728000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:p>\n                    Prognosis of patients with heart failure remains poor despite improved conventional and interventional treatment regimens. The improvement of neovascularization and repair processes by administration of bone marrow-derived cells modestly improved the recovery after acute myocardial infarction. However, circulating patient-derived cells are reduced in number and function particularly in chronic heart failure. Therefore, we tested the hypothesis whether the mobilization of circulating mononuclear proangiogenic cells (CPCs) by G-CSF may overcome some of these limitations. In the present pilot study, 32 patients with at least 3-month-old myocardial infarction were randomized to G-CSF alone (G-CSF group) or intracoronary infusion of G-CSF-mobilized and cultured CPCs into the infarct-related artery (G-CSF\/CPC group). Primary endpoint of the study was safety. Efficacy parameters included serial assessment of LV function, NT-proBNP levels, and cardiopulmonary exercise testing. G-CSF effectively mobilized circulating CD34\n                    <jats:sup>+<\/jats:sup>\n                    CD45\n                    <jats:sup>+<\/jats:sup>\n                    cells after 5 days in all patients (408 \u00b1 64%) without serious adverse events. At 3 months, NYHA class and global LV function did not show significant improvements in both treatment groups (G-CSF: \u0394LVEF 1.6 \u00b1 2.4%; p = 0.10; G-CSF\/CPC: \u0394LVEF 1.4 \u00b1 4.1%; p = 0.16). In contrast, target area contractility improved significantly in the G-CSF\/CPC group. During 5-year follow-up, one patient died after rehospitalization for worsening heart failure. Eleven patients underwent further revascularization procedures. NT-proBNP levels, cardiopulmonary exercise capacity, and NYHA class remained stable in both treatment groups. The results from our pilot trial indicate that administration of G-CSF alone or G-CSF-mobilized and cultured CPCs can be performed safely in patients with chronic ischemic heart disease. However, only minor effects on LV function, NT-proBNP levels, and NYHA classification were observed during follow-up, suggesting that the enhancement of CPCs by G-CSF alone does not substantially improve intracoronary cell therapy effects in patients with chronic ischemic heart failure.\n                  <\/jats:p>","DOI":"10.3727\/096368912x654957","type":"journal-article","created":{"date-parts":[[2012,12,20]],"date-time":"2012-12-20T08:10:56Z","timestamp":1355991056000},"page":"2325-2337","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["G-CSF Stimulation and Coronary Reinfusion of Mobilized Circulating Mononuclear Proangiogenic Cells in Patients with Chronic Ischemic Heart Disease: Five-Year Results of the TOPCARE-G-CSF Trial"],"prefix":"10.1177","volume":"21","author":[{"given":"Joerg","family":"Honold","sequence":"first","affiliation":[{"name":"Division of Cardiology, Department of Medicine III, Goethe University Frankfurt, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Fischer-Rasokat","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine III, Goethe University Frankfurt, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf","family":"Lehmann","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine III, Goethe University Frankfurt, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Leistner","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine III, Goethe University Frankfurt, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florian H.","family":"Seeger","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine III, Goethe University Frankfurt, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Volker","family":"Schachinger","sequence":"additional","affiliation":[{"name":"Department of Medicine I, Klinikum Fulda, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans","family":"Martin","sequence":"additional","affiliation":[{"name":"Division of Hematology and Oncology, Department of Medicine II, Goethe University Frankfurt, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"Dimmeler","sequence":"additional","affiliation":[{"name":"Institute for Cardiovascular Regeneration, Center of Molecular Medicine, Goethe University Frankfurt, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas M.","family":"Zeiher","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine III, Goethe University Frankfurt, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birgit","family":"Assmus","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine III, Goethe University Frankfurt, Frankfurt, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2012,11,1]]},"reference":[{"key":"bibr1-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1016\/j.ahj.2008.03.024"},{"key":"bibr2-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1093\/eurjhf\/hfq150"},{"key":"bibr3-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa051779"},{"key":"bibr4-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000043246.74879.CD"},{"key":"bibr5-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.8.11.1675"},{"key":"bibr6-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1702072"},{"key":"bibr7-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.2008.148429"},{"key":"bibr8-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1002\/ccd.22455"},{"key":"bibr9-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1016\/j.ahj.2006.09.003"},{"issue":"8","key":"bibr10-096368912X654957","doi-asserted-by":"crossref","first-page":"1712","DOI":"10.1016\/j.jacc.2006.07.044","volume":"48","author":"Engelmann M. G.","year":"2006","journal-title":"J. Am. Coll. Cardiol."},{"key":"bibr11-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000185811.71306.8b"},{"key":"bibr12-096368912X654957","doi-asserted-by":"publisher","DOI":"10.3727\/096368909X474843"},{"key":"bibr13-096368912X654957","doi-asserted-by":"publisher","DOI":"10.3727\/096368909X12483162197006"},{"key":"bibr14-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminhematol.2007.04.002"},{"key":"bibr15-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2005.01.067"},{"key":"bibr16-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1007\/s00392-008-701-6"},{"key":"bibr17-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000240248.55172.dd"},{"key":"bibr18-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67861-0"},{"issue":"2","key":"bibr19-096368912X654957","first-page":"237","volume":"153","author":"Kang H. J.","year":"2007","journal-title":"Am. Heart J."},{"issue":"9411","key":"bibr20-096368912X654957","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1016\/S0140-6736(04)15689-4","volume":"363","author":"Kang H. J.","year":"2004","journal-title":"Lancet"},{"issue":"1","key":"bibr21-096368912X654957","first-page":"I145","volume":"114","author":"Kang H. J.","year":"2006","journal-title":"Circulation"},{"key":"bibr22-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2007.01.095"},{"key":"bibr23-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.111.245993"},{"key":"bibr24-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehp374"},{"issue":"16","key":"bibr25-096368912X654957","doi-asserted-by":"crossref","first-page":"1983","DOI":"10.1161\/CIRCULATIONAHA.105.610469","volume":"113","author":"Ripa R. S.","year":"2006","journal-title":"Circulation"},{"key":"bibr26-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa060186"},{"key":"bibr27-096368912X654957","doi-asserted-by":"publisher","DOI":"10.3727\/096368909788534951"},{"key":"bibr28-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.106.684381"},{"key":"bibr29-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1007\/s00392-010-0228-5"},{"key":"bibr30-096368912X654957","doi-asserted-by":"publisher","DOI":"10.2337\/db06-0699"},{"key":"bibr31-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1161\/hh1301.093953"},{"key":"bibr32-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2010.1"},{"key":"bibr33-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2009.02.013"},{"key":"bibr34-096368912X654957","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2007.11.073"}],"container-title":["Cell Transplantation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.3727\/096368912X654957","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/full-xml\/10.3727\/096368912X654957","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.3727\/096368912X654957","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:37:15Z","timestamp":1777390635000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.sagepub.com\/doi\/10.3727\/096368912X654957"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11]]},"references-count":34,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.3727\/096368912X654957"],"URL":"https:\/\/doi.org\/10.3727\/096368912x654957","relation":{},"ISSN":["0963-6897","1555-3892"],"issn-type":[{"value":"0963-6897","type":"print"},{"value":"1555-3892","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11]]}}}