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In this study, different types of allogeneic cells including SCs, adipose-derived adult stem cells (ASCs), dental pulp stem cells (DPSCs), and the combination of SCs with ASCs or DPSCs were seeded on nerve conduits to test their efficacy in repairing a 15-mm-long critical gap defect of rat sciatic nerve. The regeneration capacity and functional recovery were evaluated by the histological staining, electrophysiology, walking track, and functional gait analysis after 8 weeks of implantation. An in vitro study was also performed to verify if the combination of cells led to syner-gistic neurotrophic effects (NGF, BDNF, and GDNF). Experimental rats receiving conduits seeded with a combination of SCs and ASCs had the greatest functional recovery, as evaluated by the walking track, functional gait, nerve conduction velocity (NCV), and histological analysis. Conduits seeded with cells were always superior to the blank conduits without cells. Regarding NCV and the number of blood vessels, conduits seeded with SCs and DPSCs exhibited better values than those seeded with DPSCs only. Results from the in vitro study confirmed the synergistic NGF production from the coculture of SCs and ASCs. It was concluded that coculture of SCs with ASCs or DPSCs in a conduit promoted peripheral nerve regeneration over a critical gap defect.<\/jats:p>","DOI":"10.3727\/096368912x658953","type":"journal-article","created":{"date-parts":[[2013,4,9]],"date-time":"2013-04-09T11:08:58Z","timestamp":1365505738000},"page":"2029-2039","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":63,"title":["Sciatic Nerve Regeneration by Cocultured Schwann Cells and Stem Cells on Microporous Nerve Conduits"],"prefix":"10.1177","volume":"22","author":[{"given":"Lien-Guo","family":"Dai","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan"},{"name":"Department of Orthopedics, Kuang Tien General Hospital, Taichung, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo-Shiang","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Kuang Tien General Hospital, Taichung, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan-Hui","family":"Hsu","sequence":"additional","affiliation":[{"name":"Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan"},{"name":"Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, Taipei, Taiwan"},{"name":"Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2013,11]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90265-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.09.043"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0979"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199811)177:2<334::AID-JCP15>3.0.CO;2-9"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0959-4388(93)90157-T"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.10.046"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.3171\/2009.8.JNS09850"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.04.022"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5768-09.2010"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2011.02.052"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(01)00190-9"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1594.2010.01105.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.05.096"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1177\/154405910208100806"},{"issue":"5","key":"e_1_3_2_16_2","first-page":"347","article-title":"Yoon E","volume":"7","author":"Gu J. 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