{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T01:09:08Z","timestamp":1777511348295,"version":"3.51.4"},"reference-count":77,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2015,8,1]],"date-time":"2015-08-01T00:00:00Z","timestamp":1438387200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:p>Efficient maintenance of the undifferentiated status of embryonic stem cells (ESCs) may be important for preparation of high-quality cell sources that can be successfully used for stem cell research and therapy. Here we tried to identify a compound that can enhance the quality of pluripotent stem cells. Treatment of ESCs and induced pluripotent stem cells (iPSCs) with 3,2\u2032-dihydroxyflavone (3,2\u2032-DHF) led to increases in cell growth, colony formation, and cell proliferation. Treatment with 3,2\u2032-DHF resulted in high expression of pluripotency markers (OCT4, SOX2, and NANOG) and significant activation (STAT3 and AKT) or suppression (GSK3\u03b2 and ERK) of self-renewal-related kinases. 3,2\u2032-DHF-treated high-quality pluripotent stem cells also showed enhanced differentiation potential. In particular, treatment of iPSCs with 3,2\u2032-DHF led to elevated expression of ectodermal differentiation markers and improved differentiation into fully matured neurons. Next, we investigated the in vivo effect of 3,2\u2032-DHF-pretreated iPSCs (3,2\u2032-DHF iPSCs) in a peripheral nerve injury model and found that transplantation of 3,2\u2032-DHF iPSCs resulted in more efficient axonal regeneration and functional recovery than in controls. Upon histopathological and gene expression analyses, we found that transplantation of 3,2\u2032-DHF iPSCs stimulated expression of cytokines, such as TNF-\u03b1, in the early phase of injury and successfully reduced convalescence time of the injured peripheral nerve, showing an effective neuroprotective property. Taken together, our data suggest that 3,2\u2032-DHF can be used for more efficient maintenance of pluripotent stem cells as well as for further applications in stem cell research and therapy.<\/jats:p>","DOI":"10.3727\/096368914x683511","type":"journal-article","created":{"date-parts":[[2014,7,31]],"date-time":"2014-07-31T20:16:09Z","timestamp":1406837769000},"page":"1511-1532","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":18,"title":["3,2\n                    <sup>\/<\/sup>\n                    -Dihydroxyflavone-Treated Pluripotent Stem Cells Show Enhanced Proliferation, Pluripotency Marker Expression, and Neuroprotective Properties"],"prefix":"10.1177","volume":"24","author":[{"given":"Dawoon","family":"Han","sequence":"first","affiliation":[{"name":"Department of Animal Biotechnology, Animal Resources Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, Gwangjin-Gu, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han Jun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Pathology, College of Veterinary Medicine, Konkuk University, Gwangjin-Gu, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye Yeon","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology, Animal Resources Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, Gwangjin-Gu, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bongwoo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology, Animal Resources Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, Gwangjin-Gu, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gwangmo","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology, Animal Resources Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, Gwangjin-Gu, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jihae","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology, Animal Resources Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, Gwangjin-Gu, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed Abdal","family":"Dayem","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology, Animal Resources Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, Gwangjin-Gu, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye-Rim","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Pathology, College of Veterinary Medicine, Konkuk University, Gwangjin-Gu, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin Hoi","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology, Animal Resources Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, Gwangjin-Gu, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung-Mi","family":"Lee","sequence":"additional","affiliation":[{"name":"Global Research Laboratory, Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyu-Shik","family":"Jeong","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Kyungpook National University, Daegu City, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sun Hee","family":"Do","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Pathology, College of Veterinary Medicine, Konkuk University, Gwangjin-Gu, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ssang-Goo","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology, Animal Resources Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, Gwangjin-Gu, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2015,8,1]]},"reference":[{"key":"bibr1-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1007\/s12640-011-9265-8"},{"key":"bibr2-096368914X683511","doi-asserted-by":"publisher","DOI":"10.2174\/157015910793358204"},{"key":"bibr3-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-198901000-00024"},{"key":"bibr4-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1447-08.2008"},{"key":"bibr5-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1007\/s11481-013-9449-5"},{"key":"bibr6-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.11.055"},{"key":"bibr7-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2009.01.014"},{"key":"bibr8-096368914X683511","doi-asserted-by":"crossref","first-page":"45","DOI":"10.2478\/v10013-011-0005-4","volume":"17","author":"Cabaj A. Z.","year":"2011","journal-title":"Pol. J. Med. Phys. Eng."},{"key":"bibr9-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0215(20000815)87:4<595::AID-IJC21>3.0.CO;2-5"},{"key":"bibr10-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00047.2007"},{"key":"bibr11-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608156103"},{"key":"bibr12-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-002-0346-6"},{"key":"bibr13-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007614613771"},{"key":"bibr14-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(98)00198-1"},{"key":"bibr15-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0333"},{"key":"bibr16-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevBiomedEng.v39.i3.40"},{"key":"bibr17-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.2011.628933"},{"key":"bibr18-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1016\/S0894-1130(98)80007-0"},{"key":"bibr19-096368914X683511","doi-asserted-by":"crossref","first-page":"526","DOI":"10.55782\/ane-2008-1720","volume":"68","author":"Figiel I.","year":"2008","journal-title":"Acta Neurobiol. Exp."},{"key":"bibr20-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-8-110"},{"key":"bibr21-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1016\/j.jocn.2009.01.031"},{"key":"bibr22-096368914X683511","first-page":"2787","volume":"13","author":"Guan R. X.","year":"2009","journal-title":"J. Clin. Rehabil. Tissue Eng. Res."},{"key":"bibr23-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1004584107"},{"key":"bibr24-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1080\/01635589309514267"},{"key":"bibr25-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0057721"},{"key":"bibr26-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1097\/00005373-200110000-00011"},{"key":"bibr27-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.04.011"},{"key":"bibr28-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/79.2.282"},{"key":"bibr29-096368914X683511","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.7.7.6164"},{"key":"bibr30-096368914X683511","first-page":"1157","volume":"36","author":"Kim J. H.","year":"2012","journal-title":"N.; Cho, S. G. Combined treatment of 3-hydroxyflavone and imatinib mesylate increases apoptotic cell death of imatinib mesylate-resistant leukemia cells. Leuk. Res."},{"key":"bibr31-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(01)00995-0"},{"key":"bibr32-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1097\/00041433-200202000-00007"},{"key":"bibr33-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.03.061"},{"key":"bibr34-096368914X683511","doi-asserted-by":"publisher","DOI":"10.2174\/187220807780809445"},{"key":"bibr35-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.30.2394"},{"key":"bibr36-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M505537200"},{"key":"bibr37-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1159\/000329973"},{"key":"bibr38-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.30.32"},{"key":"bibr39-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2006.06.006"},{"key":"bibr40-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.10-029A"},{"key":"bibr41-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1303669110"},{"key":"bibr42-096368914X683511","doi-asserted-by":"publisher","DOI":"10.5435\/00124635-200007000-00005"},{"key":"bibr43-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-008-1009-y"},{"key":"bibr44-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.09.098"},{"key":"bibr45-096368914X683511","doi-asserted-by":"publisher","DOI":"10.5114\/aoms.2011.24127"},{"key":"bibr46-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-07-2350"},{"key":"bibr47-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2007.05.014"},{"key":"bibr48-096368914X683511","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v19.i1.42"},{"issue":"1","key":"bibr49-096368914X683511","first-page":"25","volume":"18","author":"Mokry J.","year":"1999","journal-title":"Gen. Physiol. Biophys."},{"key":"bibr50-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81769-9"},{"key":"bibr51-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0b013e3181d5279e"},{"key":"bibr52-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1016\/j.ddmod.2004.09.008"},{"key":"bibr53-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0095987"},{"key":"bibr54-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhsb.2004.09.003"},{"key":"bibr55-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-4526"},{"key":"bibr56-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0404.1995.tb06996.x"},{"key":"bibr57-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2006.01.033"},{"key":"bibr58-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200317977"},{"key":"bibr59-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1189"},{"key":"bibr60-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.11.019"},{"key":"bibr61-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5391.1145"},{"key":"bibr62-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1042\/BA20070040"},{"key":"bibr63-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.407197"},{"key":"bibr64-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0270(01)00378-8"},{"key":"bibr65-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1002\/1098-2744(200006)28:2<102::AID-MC6>3.0.CO;2-2"},{"key":"bibr66-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M305529200"},{"key":"bibr67-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2202-12-52"},{"key":"bibr68-096368914X683511","doi-asserted-by":"crossref","first-page":"580752","DOI":"10.1093\/ecam\/neq062","volume":"2011","author":"Wong K. H.","year":"2011","journal-title":"(Aphyllophoromycetideae). Evid. Based Complement Alternat. Med."},{"key":"bibr69-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-012-8292-7"},{"key":"bibr70-096368914X683511","first-page":"1345","volume":"40","author":"Yamahatsu K.","year":"2012","journal-title":"Int. J. Oncol."},{"key":"bibr71-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003172"},{"key":"bibr72-096368914X683511","doi-asserted-by":"publisher","DOI":"10.2174\/1875043501104010054"},{"key":"bibr73-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200504250-00013"},{"key":"bibr74-096368914X683511","first-page":"413","volume":"21","author":"Yin F.","year":"2001","journal-title":"Anticancer Res."},{"key":"bibr75-096368914X683511","doi-asserted-by":"publisher","DOI":"10.1126\/science.1151526"},{"key":"bibr76-096368914X683511","doi-asserted-by":"publisher","DOI":"10.3109\/00207454.2010.544432"},{"key":"bibr77-096368914X683511","first-page":"1281","volume":"24","author":"Zhao Z.","year":"2010","journal-title":"Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi"}],"container-title":["Cell Transplantation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.3727\/096368914X683511","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/full-xml\/10.3727\/096368914X683511","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.3727\/096368914X683511","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:40:10Z","timestamp":1777390810000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.sagepub.com\/doi\/10.3727\/096368914X683511"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,8]]},"references-count":77,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.3727\/096368914X683511"],"URL":"https:\/\/doi.org\/10.3727\/096368914x683511","relation":{},"ISSN":["0963-6897","1555-3892"],"issn-type":[{"value":"0963-6897","type":"print"},{"value":"1555-3892","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,8]]}}}