{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T04:54:04Z","timestamp":1783486444146,"version":"3.55.0"},"reference-count":47,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:p>This study aimed to identify the beneficial effects of adipose tissue-derived mesenchymal stem cells (ASCs) and ASCs that overexpress the CTLA4Ig gene (CTLA4Ig-ASCs) on established autoimmune thyroiditis and to examine changes in clinical chemistry parameters and the presence of humoral responses upon repeated long-term administration of autologous ASCs. This study also aimed to acquire desirable results in a preclinical study by using large-sized lab animals and applying ASCs that overexpress therapeutic genes. Experimental autoimmune thyroiditis was induced by immunization with thyroglobulin. Experimental dogs were divided into five groups: (i) ASC IT + IV, (ii) ASC IV, (iii) CTLA4Ig-ASC IT + IV, (iv) CTLA4Ig-ASC IV, and (v) control IT + IV (saline only), and they received intrathyroidal (IT; 10 million cells\/250 \u03bcl saline per thyroid) administration one time or intravenous (IV; 20 million cells\/5 ml) administration seven times within a 101-day period. Blood samples were collected every week, and thyroids were harvested on days 104-106. After serial ASC or CTLA4Ig transplantation, the levels of canine thyroglobulin autoantibodies (TgAA) in serum and the infiltration of T-lymphocytes between the follicles of the thyroid glands were decreased. The expression of FoxP3 in submandibular lymph nodes was significantly increased. Repeated long-term administration of autologous ASCs or CTLA4Ig-ASCs did not generate changes in clinical chemistry parameters or humoral responses. The TgAA test can detect autoimmune thyroiditis years before clinical signs of hypothyroidism occur. Thus, ASC and CTLA4Ig-ASC transplantation in that period can be attractive candidates to ameliorate autoimmune thyroiditis and prevent the development of hypothyroidism.<\/jats:p>","DOI":"10.3727\/096368914x685122","type":"journal-article","created":{"date-parts":[[2015,10,29]],"date-time":"2015-10-29T19:51:34Z","timestamp":1446148294000},"page":"2221-2236","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Therapeutic Effects of\n                    <i>CTLA4Ig<\/i>\n                    Gene-Transduced Adipose Tissue-Derived Mesenchymal Stem Cell Transplantation on Established Autoimmune Thyroiditis"],"prefix":"10.1177","volume":"24","author":[{"given":"Eun Wha","family":"Choi","sequence":"first","affiliation":[{"name":"Laboratory Animal Research Center, Samsung Biomedical Research Institute, Seoul, Republic of Korea"},{"name":"School of Medicine, Sungkyunkwan University, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung Min","family":"Lee","sequence":"additional","affiliation":[{"name":"Laboratory Animal Research Center, Samsung Biomedical Research Institute, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee Woo","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jehoon","family":"Yang","sequence":"additional","affiliation":[{"name":"Laboratory Animal Research Center, Samsung Biomedical Research Institute, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hwa Young","family":"Youn","sequence":"additional","affiliation":[{"name":"Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2015,11,1]]},"reference":[{"key":"bibr1-096368914X685122","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-04-1559"},{"key":"bibr2-096368914X685122","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199801)28:01<201::AID-IMMU201>3.0.CO;2-N"},{"key":"bibr3-096368914X685122","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.4.1681"},{"key":"bibr4-096368914X685122","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2008.03.002"},{"key":"bibr5-096368914X685122","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2006.12.002"},{"key":"bibr6-096368914X685122","first-page":"1419","volume-title":"Textbook of veterinary internal medicine","author":"Catharine R. 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