{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T01:14:13Z","timestamp":1777511653001,"version":"3.51.4"},"reference-count":33,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cell Transplant"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:p>\n                    CD34\n                    <jats:sup>+<\/jats:sup>\n                    progenitor cells are growing in use for vascular repair. However, in diabetic individuals with cardiovascular diseases, these cells have dysfunctional engraftment capabilities, which compromise their use for autologous cell therapy. The thrombospondin-1-derived peptide RFYVVMWK has previously been reported to stimulate cell adhesiveness through CD47 and integrin activation pathways. Our aim was to test whether RFYVVMWK preconditioning could modulate CD34\n                    <jats:sup>+<\/jats:sup>\n                    cell phenotype and enhance its proadhesive properties in diabetic patients. Peripheral blood mononuclear CD34\n                    <jats:sup>+<\/jats:sup>\n                    cells isolated from 40 atherosclerotic patients with type 2 diabetes (T2D; n = 20) or without (non-T2D; n = 20) were preconditioned with 30 \u03bcM RFYVVMWK or truncated peptide RFYVVM. CD34\n                    <jats:sup>+<\/jats:sup>\n                    cell adhesion was assessed on a vitronectin\u2013collagen matrix and on TNF-\u03b1 or IL-1\u03b2-stimulated HUVEC monolayers. Adhesion receptors, platelet\/CD34\n                    <jats:sup>+<\/jats:sup>\n                    cell conjugates, and cell viability were analyzed by flow cytometry and confocal microscopy. RFYVVMWK increased the adhesion of T2D CD34\n                    <jats:sup>+<\/jats:sup>\n                    cells by eightfold to the vitronectin\u2013collagen matrix ( p &lt; 0.001) corresponding to a threefold increase compared to unstimulated non-T2D CD34\n                    <jats:sup>+<\/jats:sup>\n                    cells. The peptide induced the formation of platelet\/CD34\n                    <jats:sup>+<\/jats:sup>\n                    conjugates and increased the expression of TSP-1, CD29, CD51\/CD61, and CD62P in both T2D and non-T2D cells. However, RFYVVMWK treatment did not affect the viability\/apoptosis of CD34\n                    <jats:sup>+<\/jats:sup>\n                    progenitor cells. In conclusion, priming CD34\n                    <jats:sup>+<\/jats:sup>\n                    cells with RFYVVMWK may enhance their vascular engraftment during autologous proangiogenic cell therapy.\n                  <\/jats:p>","DOI":"10.3727\/096368916x693329","type":"journal-article","created":{"date-parts":[[2016,10,7]],"date-time":"2016-10-07T00:38:40Z","timestamp":1475800720000},"page":"327-337","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Thrombospondin-1-Derived Peptide RFYVVMWK Improves the Adhesive Phenotype of CD34\n                    <sup>+<\/sup>\n                    Cells from Atherosclerotic Patients with Type 2 Diabetes"],"prefix":"10.1177","volume":"26","author":[{"given":"Sylvie","family":"Cointe","sequence":"first","affiliation":[{"name":"VRCM, UMR-S1076, Aix-Marseille Universit\u00e9, INSERM, UFR de Pharmacie, Marseille, France"},{"name":"Hematology and Vascular Biology Department, CHU La Conception, Assistance Publique-H\u00f4pitaux de Marseille, Marseille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c9ric","family":"Rh\u00e9aume","sequence":"additional","affiliation":[{"name":"Department of Medicine, Universit\u00e9 de Montr\u00e9al; Montreal Heart Institute Research Center, Montreal, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Martel","sequence":"additional","affiliation":[{"name":"Department of Medicine, Universit\u00e9 de Montr\u00e9al; Montreal Heart Institute Research Center, Montreal, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Blanc-Brude","sequence":"additional","affiliation":[{"name":"PARCC UMRs970, H\u00f4pital Europ\u00e9en Georges Pompidou, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evemie","family":"Dub\u00e9","sequence":"additional","affiliation":[{"name":"CHU Sainte-Justine, University of Montreal, Montreal, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florence","family":"Sabatier","sequence":"additional","affiliation":[{"name":"VRCM, UMR-S1076, Aix-Marseille Universit\u00e9, INSERM, UFR de Pharmacie, Marseille, France"},{"name":"Hematology and Vascular Biology Department, CHU La Conception, Assistance Publique-H\u00f4pitaux de Marseille, Marseille, France"},{"name":"Cell Therapy Department, CHU La Conception, Assistance Publique-H\u00f4pitaux de Marseille, INSERM CBT-1409, Marseille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7oise","family":"Dignat-George","sequence":"additional","affiliation":[{"name":"VRCM, UMR-S1076, Aix-Marseille Universit\u00e9, INSERM, UFR de Pharmacie, Marseille, France"},{"name":"Hematology and Vascular Biology Department, CHU La Conception, Assistance Publique-H\u00f4pitaux de Marseille, Marseille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Claude","family":"Tardif","sequence":"additional","affiliation":[{"name":"Department of Medicine, Universit\u00e9 de Montr\u00e9al; Montreal Heart Institute Research Center, Montreal, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnaud","family":"Bonnefoy","sequence":"additional","affiliation":[{"name":"CHU Sainte-Justine, University of Montreal, Montreal, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,2,1]]},"reference":[{"key":"bibr1-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2008.10.009"},{"key":"bibr2-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1038\/nm0603-702"},{"key":"bibr3-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.221762"},{"key":"bibr4-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCINTERVENTIONS.114.001436"},{"key":"bibr5-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000039526.42991.93"},{"key":"bibr6-096368916X693329","doi-asserted-by":"publisher","DOI":"10.2337\/dc12-1084"},{"key":"bibr7-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2445"},{"key":"bibr8-096368916X693329","doi-asserted-by":"publisher","DOI":"10.2337\/db11-1768"},{"key":"bibr9-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1186\/2052-8426-1-4"},{"key":"bibr10-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.107.714691"},{"key":"bibr11-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1160\/TH06-05-0250"},{"key":"bibr12-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-007-7487-y"},{"key":"bibr13-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2008.07.015"},{"key":"bibr14-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.135.2.533"},{"key":"bibr15-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.23.14740"},{"key":"bibr16-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.147.2.389"},{"key":"bibr17-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M206849200"},{"key":"bibr20-096368916X693329","doi-asserted-by":"publisher","DOI":"10.2337\/db06-1254"},{"key":"bibr21-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1126\/science.7512751"},{"key":"bibr22-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.4.882"},{"key":"bibr23-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118808"},{"key":"bibr24-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2004.08.019"},{"key":"bibr25-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1702136"},{"key":"bibr26-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.2.1323"},{"key":"bibr27-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1172\/JCI28338"},{"key":"bibr28-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1038\/mtm.2016.25"},{"key":"bibr29-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1007\/s00392-009-0021-5"},{"key":"bibr30-096368916X693329","doi-asserted-by":"publisher","DOI":"10.2337\/db13-0894"},{"key":"bibr31-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1005550"},{"key":"bibr32-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2010.01.036"},{"key":"bibr33-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1186\/s12933-016-0408-3"},{"key":"bibr34-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdiacomp.2013.04.007"},{"key":"bibr35-096368916X693329","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2015.08.035"}],"container-title":["Cell Transplantation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.3727\/096368916X693329","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/full-xml\/10.3727\/096368916X693329","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.sagepub.com\/doi\/pdf\/10.3727\/096368916X693329","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:41:15Z","timestamp":1777390875000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.sagepub.com\/doi\/10.3727\/096368916X693329"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,2]]}},"alternative-id":["10.3727\/096368916X693329"],"URL":"https:\/\/doi.org\/10.3727\/096368916x693329","relation":{},"ISSN":["0963-6897","1555-3892"],"issn-type":[{"value":"0963-6897","type":"print"},{"value":"1555-3892","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2]]}}}