{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T18:35:10Z","timestamp":1789238110762,"version":"build-2803163510"},"reference-count":38,"publisher":"Open Exploration Publishing","license":[{"start":{"date-parts":[[2022,10,27]],"date-time":"2022-10-27T00:00:00Z","timestamp":1666828800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/doi.org\/10.37349\/Open_Exploration_Crossmark_Policy"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Aim: Diagnostic laboratories are progressively introducing next-generation sequencing (NGS) technologies in the routine workflow to meet the increasing clinical need for comprehensive molecular characterization in cancer patients for diagnosis and precision medicine, including fusion-transcripts detection. Nevertheless, the low quality of messenger RNA (mRNA) extracted from formalin-fixed paraffin-embedded (FFPE) samples may affect the transition from traditional single-gene testing approaches [like fluorescence in situ hybridization (FISH), immunohistochemistry (IHC), or polymerase chain reaction (PCR)] to NGS. The present study is aimed at assessing the overall accuracy of RNA fusion transcripts detection by NGS analysis in FFPE samples in real-world diagnostics.\nMethods: Herein, NGS data from 190 soft tissue tumors (STTs) and carcinoma cases, discussed in the context of the institutional Molecular Tumor Board, are reported and analyzed by FusionPlex\u00a9 Solid tumor kit through the manufacturer\u2019s pipeline and by two well-known fast and accurate open-source tools [Arriba (ARR) and spliced transcripts alignment to reference (STAR)-fusion (SFU)].\nResults: The combination of FusionPlex\u00a9 Solid tumor with ArcherDX\u00ae Analysis suite (ADx) analysis package has been proven to be sensitive and specific in STT samples, while partial loss of sensitivity has been found in carcinoma specimens.\nConclusions: Albeit ARR and SFU showed lower sensitivity, the use of additional fusion-detection tools can contribute to reinforcing or extending the output obtained by ADx, particularly in the case of low-quality input data. Overall, our results sustain the clinical use of NGS for the detection of fusion transcripts in FFPE material.<\/jats:p>","DOI":"10.37349\/etat.2022.00102","type":"journal-article","created":{"date-parts":[[2022,10,27]],"date-time":"2022-10-27T05:49:49Z","timestamp":1666849789000},"page":"582-597","update-policy":"https:\/\/doi.org\/10.37349\/open_exploration_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Targeted RNA-sequencing analysis for fusion transcripts detection in tumor diagnostics: assessment of bioinformatic tools reliability in FFPE samples"],"prefix":"10.37349","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6572-614X","authenticated-orcid":false,"given":"Iolanda","family":"Capone","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0729-1288","authenticated-orcid":false,"given":"Fabio","family":"Bozzi","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6743-1935","authenticated-orcid":false,"given":"Gian Paolo","family":"Dagrada","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9231-1281","authenticated-orcid":false,"given":"Paolo","family":"Verderio","sequence":"additional","affiliation":[{"name":"Department of Applied Research and Technological Development, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2052-8104","authenticated-orcid":false,"given":"Elena","family":"Conca","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5808-0387","authenticated-orcid":false,"given":"Adele","family":"Busico","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9462-0336","authenticated-orcid":false,"given":"Maria Adele","family":"Testi","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1459-384X","authenticated-orcid":false,"given":"Valentina","family":"Monti","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3968-5011","authenticated-orcid":false,"given":"Matteo","family":"Duca","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0287-9787","authenticated-orcid":false,"given":"Claudia","family":"Proto","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9560-1092","authenticated-orcid":false,"given":"Silvia","family":"Damian","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2078-9988","authenticated-orcid":false,"given":"Alberta","family":"Piccolo","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4406-8245","authenticated-orcid":false,"given":"Federica","family":"Perrone","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3819-8687","authenticated-orcid":false,"given":"Elena","family":"Tamborini","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0440-3221","authenticated-orcid":false,"given":"Andrea","family":"Devecchi","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6158-210X","authenticated-orcid":false,"given":"Paola","family":"Collini","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1425-3354","authenticated-orcid":false,"given":"Daniele","family":"Lorenzini","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9827-3976","authenticated-orcid":false,"given":"Andrea","family":"Vingiani","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy; Department of Oncology and Hemato-oncology, University of Milan, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0582-6170","authenticated-orcid":false,"given":"Luca","family":"Agnelli","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy; Department of Medical Oncology, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7963-7172","authenticated-orcid":false,"given":"Giancarlo","family":"Pruneri","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy; Department of Oncology and Hemato-oncology, University of Milan, 20133 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"21850","published-online":{"date-parts":[[2022,10,27]]},"reference":[{"key":"ref0","doi-asserted-by":"publisher","unstructured":"Kumar-Sinha C, Kalyana-Sundaram S, Chinnaiyan AM. 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Curr Drug Targets. 2017;18:13\u201326.","DOI":"10.2174\/1389450116666150330114441"},{"key":"ref4","doi-asserted-by":"publisher","unstructured":"List of cleared or approved companion diagnostic devices (in vitro and imaging tools) [Internet]. U.S. Food & Drug Administration; c2022 [cited 2022 Aug 1]. Available from: https:\/\/www.fda.gov\/medical-devices\/in-vitro-diagnostics\/list-cleared-or-approved-companion-diagnostic-devices-in-vitro-and-imaging-tools","DOI":"10.1093\/cid\/cir046"},{"key":"ref5","doi-asserted-by":"publisher","unstructured":"Mosele F, Remon J, Mateo J, Westphalen CB, Barlesi F, Lolkema MP et al. Recommendations for the use of next-generation sequencing (NGS) for patients with metastatic cancers: a report from the ESMO Precision Medicine Working Group. Ann Oncol. 2020;31:1491\u2013505.","DOI":"10.1016\/j.annonc.2020.07.014"},{"key":"ref6","doi-asserted-by":"publisher","unstructured":"Pruneri G, De Braud F, Sapino A, Aglietta M, Vecchione A, Giusti R et al. 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Am J Surg Pathol. 2015;39:365\u201373.","DOI":"10.1097\/pas.0000000000000338"},{"key":"ref13","doi-asserted-by":"publisher","unstructured":"Brassesco MS, Cortez MA, Valera ET, Engel EE, Nogueira-Barbosa MH, Becker AP, et al. Cryptic SYT\/SXX1 fusion gene in high-grade biphasic synovial sarcoma with unique complex rearrangement and extensive BCL2 overexpression. Cancer Genet Cytogenet. 2010;196:189\u201393.","DOI":"10.1016\/j.cancergencyto.2009.10.001"},{"key":"ref14","doi-asserted-by":"publisher","unstructured":"Torres L, Lisboa S, Cerveira N, Lopes JM, Lopes C, Teixeira MR. Cryptic chromosome rearrangement resulting in SYT-SSX2 fusion gene in a monophasic synovial sarcoma. Cancer Genet Cytogenet. 2008;187:45\u20139.","DOI":"10.1016\/j.cancergencyto.2008.07.008"},{"key":"ref15","doi-asserted-by":"publisher","unstructured":"Yoshida A, Arai Y, Kobayashi E, Yonemori K, Ogura K, Hama N, et al. CIC break-apart fluorescence in-situ hybridization misses a subset of CIC-DUX4 sarcomas: a clinicopathological and molecular study. Histopathology. 2017;71:461\u20139.","DOI":"10.1111\/his.13252"},{"key":"ref16","doi-asserted-by":"publisher","unstructured":"Soft tissue and bone tumours\u2013WHO classification of tumours, 5th edition, volume 3 [Internet]. Lyon: International agency for research on Cancer (IARC); c2022 [cited 2022 Aug 1]. Available from: https:\/\/publications.iarc.fr\/Book-And-Report-Series\/Who-Classification-Of-Tumours\/Soft-Tissue-And-Bone-Tumours-2020","DOI":"10.1002\/9781118695647.ch4"},{"key":"ref17","doi-asserted-by":"publisher","unstructured":"Davies KD, Le AT, Theodoro MF, Skokan MC, Aisner DL, Berge EM, et al. Identifying and targeting ROS1 gene fusions in non-small cell lung cancer. Clin Cancer Res. 2012;18:4570\u20139.","DOI":"10.1158\/1078-0432.ccr-12-0550"},{"key":"ref18","doi-asserted-by":"publisher","unstructured":"Lee J, Lee SE, Kang SY, Do IG, Lee S, Ha SY, et al. Identification of ROS1 rearrangement in gastric adenocarcinoma. Cancer. 2013;119:1627\u201335.","DOI":"10.1002\/cncr.27967"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Dickson BC, Antonescu CR, Argyris PP, Bilodeau EA, Bullock MJ, Freedman PD, et al. Ectomesenchymal chondromyxoid tumor: a neoplasm characterized by recurrent RREB1-MKL2 fusions. Am J Surg Pathol. 2018;42:1297\u2013305.","DOI":"10.1097\/PAS.0000000000001096"},{"key":"ref20","doi-asserted-by":"publisher","unstructured":"Siegfried A, Romary C, Escudi\u00e9 F, Nicaise Y, Grand D, Rochaix P, et al. RREB1-MKL2 fusion in biphenotypic \u201coropharyngeal\u201d sarcoma: new entity or part of the spectrum of biphenotypic sinonasal sarcomas? Genes Chromosomes Cancer. 2018;57:203\u201310.","DOI":"10.1002\/gcc.22521"},{"key":"ref21","doi-asserted-by":"publisher","unstructured":"Thoracic tumours\u2013WHO classification of tumours, 5th edition, volume 5 [Internet]. Lyon: International agency for research on Cancer (IARC); c2022 [cited 2022 Aug 1]. Available from: https:\/\/publications.iarc.fr\/Book-And-Report-Series\/Who-Classification-Of-Tumours\/Thoracic-Tumours-2021","DOI":"10.1186\/bcr788"},{"key":"ref22","unstructured":"Maraka S, Janku F. BRAF alterations in primary brain tumors. Discov Med. 2018;26:51\u201360."},{"key":"ref23","doi-asserted-by":"publisher","unstructured":"Liu M, Chen P, Hu HY, Ou-Yang DJ, Khushbu RA, Tan HL, et al. Kinase gene fusions: roles and therapeutic value in progressive and refractory papillary thyroid cancer. J Cancer Res Clin Oncol. 2021;147:323\u201337.","DOI":"10.1007\/s00432-020-03491-5"},{"key":"ref24","doi-asserted-by":"publisher","unstructured":"Soda M, Choi YL, Enomoto M, Takada S, Yamashita Y, Ishikawa S, et al. Identification of the transforming EML4-ALK fusion gene in non-small-cell lung cancer. Nature. 2007;448:561\u20136.","DOI":"10.1038\/nature05945"},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"Demetri GD, Antonescu CR, Bjerkehagen B, Bov\u00e9e JVMG, Boye K, Chac\u00f3n M, et al. Diagnosis and management of tropomyosin receptor kinase (TRK) fusion sarcomas: expert recommendations from the World Sarcoma Network. Ann Oncol. 2020;31:1506\u201317.","DOI":"10.1016\/j.annonc.2020.08.2232"},{"key":"ref26","doi-asserted-by":"publisher","unstructured":"Bubendorf L, B\u00fcttner R, Al-Dayel F, Dietel M, Elmberger G, Kerr K, et al. Testing for ROS1 in non-small cell lung cancer: a review with recommendations. Virchows Arch. 2016;469:489\u2013503.","DOI":"10.1007\/s00428-016-2000-3"},{"key":"ref27","doi-asserted-by":"crossref","unstructured":"Lee SE, Lee B, Hong M, Song JY, Jung K, Lira ME, et al. Comprehensive analysis of RET and ROS1 rearrangement in lung adenocarcinoma. Mod Pathol. 2015;28:468\u201379.","DOI":"10.1038\/modpathol.2014.107"},{"key":"ref28","doi-asserted-by":"publisher","unstructured":"Thompson ER, Lim KJC, Kuzich JA, McBean M, Westerman D, Tam CS, et al. Detection of an IGH-BRAF fusion in a patient with BRAF Val600Glu negative hairy cell leukemia. Leuk Lymphoma. 2020;61:2024\u20136.","DOI":"10.1080\/10428194.2020.1753045"},{"key":"ref29","doi-asserted-by":"publisher","unstructured":"Kannan S, Lock I, Ozenberger BB, Jones KB. Genetic drivers and cells of origin in sarcomagenesis. J Pathol. 2021;254:474\u201393.","DOI":"10.1002\/path.5617"},{"key":"ref30","doi-asserted-by":"publisher","unstructured":"Le Loarer F, Cleven AHG, Bouvier C, Castex MP, Romagosa C, Moreau A, et al. A subset of epithelioid and spindle cell rhabdomyosarcomas is associated with TFCP2 fusions and common ALK upregulation. Mod Pathol. 2020;33:404\u201319.","DOI":"10.1038\/s41379-019-0323-8"},{"key":"ref31","doi-asserted-by":"publisher","unstructured":"Xu B, Suurmeijer AJH, Agaram NP, Zhang L, Antonescu CR. Head and neck rhabdomyosarcoma with TFCP2 fusions and ALK overexpression: a clinicopathological and molecular analysis of 11 cases. Histopathology. 2021;79:347\u201357.","DOI":"10.1111\/his.14323"},{"key":"ref32","doi-asserted-by":"publisher","unstructured":"Fukuhara S, Nomoto J, Kim SW, Taniguchi H, Miyagi Maeshima A, Tobinai K, et al. Partial deletion of the ALK gene in ALK-positive anaplastic large cell lymphoma. Hematol Oncol. 2018;36:150\u20138.","DOI":"10.1002\/hon.2455"},{"key":"ref33","doi-asserted-by":"crossref","unstructured":"Haas BJ, Zody MC. Advancing RNA-Seq analysis. Nat Biotechnol. 2010;28:421\u20133.","DOI":"10.1038\/nbt0510-421"},{"key":"ref34","doi-asserted-by":"publisher","unstructured":"Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, et al. STAR: ultrafast universal RNA-seq aligner. Bioinformatics. 2013;29:15\u201321.","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"ref35","doi-asserted-by":"publisher","unstructured":"Heydt C, W\u00f6lwer CB, Velazquez Camacho O, Wagener-Ryczek S, Pappesch R, Siemanowski J, et al. Detection of gene fusions using targeted next-generation sequencing: a comparative evaluation. BMC Med Genomics. 2021;14:62.","DOI":"10.1186\/s12920-021-00909-y"},{"key":"ref36","doi-asserted-by":"publisher","unstructured":"Davies KD, Le AT, Sheren J, Nijmeh H, Gowan K, Jones KL, et al. Comparison of molecular testing modalities for detection of ROS1 rearrangements in a cohort of positive patient samples. J Thorac Oncol. 2018;13:1474\u201382.","DOI":"10.1016\/j.jtho.2018.05.041"},{"key":"ref37","doi-asserted-by":"publisher","unstructured":"Kuang Y, Xu P, Wang J, Zheng Y, Sun X, Li Z, et al. Detecting ALK rearrangement with RT-PCR: a reliable approach compared with next-generation sequencing in patients with NSCLC. Mol Diagn Ther. 2021;25:487\u201394.","DOI":"10.1007\/s40291-021-00532-8"}],"container-title":["Exploration of Targeted Anti-tumor Therapy"],"original-title":[],"link":[{"URL":"https:\/\/www.explorationpub.com\/Journals\/etat\/Article\/1002102","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,27]],"date-time":"2022-10-27T05:49:57Z","timestamp":1666849797000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.explorationpub.com\/Journals\/etat\/Article\/1002102"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,27]]},"references-count":38,"URL":"https:\/\/doi.org\/10.37349\/etat.2022.00102","archive":["Portico","Portico"],"relation":{},"ISSN":["2692-3114"],"issn-type":[{"value":"2692-3114","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,27]]},"assertion":[{"value":"2022-4-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-8-2","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-27","order":2,"name":"published_online","label":"Published Online","group":{"name":"publication_history","label":"Publication History"}}]}}