{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T05:50:47Z","timestamp":1785909047707,"version":"3.56.0"},"reference-count":42,"publisher":"Open Exploration Publishing","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Explor Target Antitumor Ther"],"accepted":{"date-parts":[[2026,7,23]]},"abstract":"<jats:p xml:lang=\"en\">Aim: Breast cancer remains a major cause of cancer-related mortality in women, particularly in advanced stages where therapeutic options are limited. While immune checkpoint inhibitors (ICIs) have improved outcomes in a subset of patients, many do not respond, highlighting the need for alternative immunotherapeutic strategies. This study evaluated the effect of dendritic cell (DC)-based immunotherapy on tumor growth and on the inflammatory profile of peritoneal myeloid cells in a 4T1 murine breast cancer model. Methods: BALB\/c mice bearing 4T1 breast tumors were treated with bone marrow-derived DC-based immunotherapy. Tumor volume was monitored over time, and CD14+ cells obtained from peritoneal lavage were analyzed by flow cytometry for the cytokines IL-12, IL-17, and TNF-\u03b1 and the transcription factors ROR\u03b3T and GATA3. Results: DC-based immunotherapy was associated with a non-significant trend toward reduced tumor volume and a marked suppression of key proinflammatory cytokines: IL-12 (P &lt; 0.0005), IL-17 (P &lt; 0.0001), and TNF-\u03b1 (P &lt; 0.0001). Expression of the transcription factors ROR\u03b3T and GATA3, associated with Th17 and Th2 differentiation, was also downregulated (P &lt; 0.0001). These immunological effects were observed in CD14+ myeloid cells from the peritoneal compartment. Conclusions: DC-based immunotherapy modulates the systemic\/peritoneal inflammatory profile and attenuates tumor-promoting inflammation. This strategy may offer therapeutic benefit for patients with breast cancer who are unresponsive to conventional or ICI-based treatments and supports its further evaluation in translational studies.<\/jats:p>","DOI":"10.37349\/etat.2026.1002393","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T05:30:50Z","timestamp":1785907850000},"update-policy":"https:\/\/doi.org\/10.37349\/open_exploration_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dendritic cell-based immunotherapy modulates the systemic inflammatory profile in a 4T1 breast cancer model"],"prefix":"10.37349","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7293-2937","authenticated-orcid":false,"given":"Tauana Christina","family":"Dias","sequence":"first","affiliation":[{"name":"Oncology Research Institute (Instituto de Pesquisa em Oncologia, IPON), Federal University of the Tri\u00e2ngulo Mineiro (Universidade Federal do Tri\u00e2ngulo Mineiro, UFTM), Uberaba 38025-350, Minas Gerais, Brazil; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3556-9849","authenticated-orcid":false,"given":"J\u00e9ssica Ferreira","family":"Vieira","sequence":"additional","affiliation":[{"name":"Oncology Research Institute (Instituto de Pesquisa em Oncologia, IPON), Federal University of the Tri\u00e2ngulo Mineiro (Universidade Federal do Tri\u00e2ngulo Mineiro, UFTM), Uberaba 38025-350, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8312-9269","authenticated-orcid":false,"given":"Katiane","family":"Tostes","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6691-1144","authenticated-orcid":false,"given":"Polyana Barbosa","family":"Silva","sequence":"additional","affiliation":[{"name":"Oncology Research Institute (Instituto de Pesquisa em Oncologia, IPON), Federal University of the Tri\u00e2ngulo Mineiro (Universidade Federal do Tri\u00e2ngulo Mineiro, UFTM), Uberaba 38025-350, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8961-1777","authenticated-orcid":false,"given":"Angela Maria Moed","family":"Lopes","sequence":"additional","affiliation":[{"name":"Oncology Research Institute (Instituto de Pesquisa em Oncologia, IPON), Federal University of the Tri\u00e2ngulo Mineiro (Universidade Federal do Tri\u00e2ngulo Mineiro, UFTM), Uberaba 38025-350, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8500-1819","authenticated-orcid":false,"given":"Gabriela Karam","family":"Rebolho","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4014-1345","authenticated-orcid":false,"given":"Eddie Fernando Candido","family":"Murta","sequence":"additional","affiliation":[{"name":"Oncology Research Institute (Instituto de Pesquisa em Oncologia, IPON), Federal University of the Tri\u00e2ngulo Mineiro (Universidade Federal do Tri\u00e2ngulo Mineiro, UFTM), Uberaba 38025-350, Minas Gerais, Brazil; Department of Gynecology and Obstetrics, UFTM, Uberaba 38025-440, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8230-1218","authenticated-orcid":false,"given":"Lidia Maria Rebolho Batista","family":"Arantes","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0842-8805","authenticated-orcid":false,"given":"M\u00e1rcia Antoniazi","family":"Michelin","sequence":"additional","affiliation":[{"name":"Oncology Research Institute (Instituto de Pesquisa em Oncologia, IPON), Federal University of the Tri\u00e2ngulo Mineiro (Universidade Federal do Tri\u00e2ngulo Mineiro, UFTM), Uberaba 38025-350, Minas Gerais, Brazil; Immunology Discipline, UFTM, Uberaba 38025-050, Minas Gerais, 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