{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T03:15:05Z","timestamp":1790651705449,"version":"4.1.0"},"reference-count":81,"publisher":"Baishideng Publishing Group Inc.","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["World J Gastroenterol"],"abstract":"<jats:p>BACKGROUND<\/jats:p>\n          <jats:p>Bletilla striata  polysaccharides (BSP) have antioxidant, immune regulation, and anti-fibrotic activities. However, the therapeutic effect and mechanisms underlying the action of BSP in metabolic dysfunction-associated steatotic liver disease (MASLD) have not been fully understood.<\/jats:p>\n          <jats:p>AIM<\/jats:p>\n          <jats:p>To investigate the therapeutic effects and mechanisms of BSP on MASLD by centering on the hepatocyte nuclear factor kappa B p65 (RelA)\/hepatocyte nuclear factor-1 alpha (HNF1\u03b1) signaling.<\/jats:p>\n          <jats:p>METHODS<\/jats:p>\n          <jats:p>A mouse model of MASLD was induced by feeding with a high-fat-diet (HFD) and a hepatocyte model of steatosis was induced by treatment with sodium oleate (SO) and sodium palmitate (SP). The therapeutic effects of BSP on MASLD were examined in vivo  and in vitro . The mechanisms underlying the action of BSP were analyzed for their effect on lipid metabolism disorder, endoplasmic reticulum (ER) stress, and the RelA\/HNF1\u03b1 signaling.<\/jats:p>\n          <jats:p>RESULTS<\/jats:p>\n          <jats:p>HFD feeding reduced hepatocyte RelA and HNF1\u03b1 expression, induced ER stress, lipid metabolism disorder, and necroptosis in mice, which were significantly mitigated by treatment with BSP. Furthermore, treatment with BSP or BSP-containing conditional rat serum significantly attenuated the sodium oleate\/sodium palmitate (SO\/SP)-induced hepatocyte steatosis by decreasing lipid accumulation, and lipid peroxidation, and enhancing the expression of RelA, and HNF1\u03b1. The therapeutic effects of BSP on MASLD were partially abrogated by RELA  silencing in mice and RELA  knockout in hepatocytes. RELA  silencing or knockout significantly down-regulated HNF1\u03b1 expression, and remodeled ER stress and oxidative stress responses during hepatic steatosis.<\/jats:p>\n          <jats:p>CONCLUSION<\/jats:p>\n          <jats:p>Treatment with BSP ameliorates MASLD, associated with enhancing the RelA\/HNF1\u03b1 signaling, remodeling ER stress and oxidative stress responses in hepatocytes.<\/jats:p>","DOI":"10.3748\/wjg.v31.i4.93179","type":"journal-article","created":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T03:08:31Z","timestamp":1735528111000},"source":"Crossref","is-referenced-by-count":4,"title":["<i>Bletilla striata<\/i> polysaccharides alleviate metabolic dysfunction-associated steatotic liver disease through enhancing hepatocyte RelA\/ HNF1\u03b1 signaling"],"prefix":"10.3748","volume":"31","author":[{"given":"Yi-Huai","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Li","family":"Ou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Lian","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Fen","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aikedaimu","family":"Abudukeremu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Xue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mao-Yuan","family":"Mu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Wei","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De-Lin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan-Yu","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Qun","family":"Guan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2060","published-online":{"date-parts":[[2025,1,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2020.03.039"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2023.11.007"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v22.i4.1664"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1210\/clinem\/dgac321"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2021.11.038"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(19)30039-1"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.10.033"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1007\/s12072-020-10094-2"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-06161-9"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.3390\/cells12182296"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2016.125"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.3390\/cells9030627"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1002\/hep.24001"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.30247"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/6412916"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.3350\/cmh.2020.0173"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1055\/s-0039-1681032"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1080\/14728222.2022.2170780"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.06.008"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1002\/hep.24396"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2021.01.042"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/j.aohep.2019.05.008"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/8717565"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/s1097-2765(03)00105-9"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3790"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202101991"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.2174\/1874467212666190930144349"},{"key":"B28","doi-asserted-by":"crossref","unstructured":"Pontoglio M. Hepatocyte nuclear factor 1, a transcription factor at the crossroads of glucose homeostasis. J Am Soc Nephrol 2000; S140-S143","DOI":"10.1681\/ASN.V11suppl_2s140"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-15846-8"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.12689"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.4093\/dmj.2019.0042"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.117401"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.10.201"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2022.02.073"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2013.10.008"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2019.05.047"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2020.116620"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2018.6430"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2023.120781"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1039\/d1fo03899k"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.7754\/clin.lab.2015.150521"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.4149\/neo_2021_210205N180"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.378"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2022.05.006"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v28.i26.3201"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines10010127"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.133"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2021.2002109"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22010066"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.125407"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2016.04.081"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2021.118149"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2016.05.002"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2020.104942"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.810143"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1080\/17512433.2022.2121700"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2022.842098"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5271"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24021502"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-00312-6"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00132-19"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-27452-9"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.02.081"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2015.526"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M025130"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M004388200"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R056705"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2016.11.006"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2017.07.003"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-03264-5"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/2626090"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1172\/JCI37007"},{"key":"B73","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2013.437"},{"key":"B74","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-013-2855-7"},{"key":"B75","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2021.112637"},{"key":"B76","doi-asserted-by":"publisher","DOI":"10.1080\/713609312"},{"key":"B77","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2017.05.032"},{"key":"B78","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2014.03.011"},{"key":"B79","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2009.03.013"},{"key":"B80","doi-asserted-by":"publisher","DOI":"10.1631\/jzus.B0820025"},{"key":"B81","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1105335"}],"container-title":["World Journal of Gastroenterology"],"original-title":[],"link":[{"URL":"https:\/\/www.wjgnet.com\/1007-9327\/full\/v31\/i4\/93179.htm","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.wjgnet.com\/1007-9327\/full\/v31\/i4\/93179.htm","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T03:08:39Z","timestamp":1735528119000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.wjgnet.com\/1007-9327\/full\/v31\/i4\/93179.htm"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,28]]},"references-count":81,"journal-issue":{"issue":"4"},"alternative-id":["93179"],"URL":"https:\/\/doi.org\/10.3748\/wjg.v31.i4.93179","relation":{},"ISSN":["1007-9327"],"issn-type":[{"value":"1007-9327","type":"print"}],"subject":[],"published":{"date-parts":[[2025,1,28]]}}}